N-glycan trimming by glucosidase II is essential for Arabidopsis development

Soussillane, Pravina; D’Alessio, Cecilia; Paccalet, Thomas; Fitchette, Anne‐Catherine; Parodi, Armando J.; Williamson, R. E.; Plasson, Carole; Faye, Loı̈c; Gomord, Véronique

doi:10.1007/s10719-008-9201-1

Cited by 39 publications

(36 citation statements)

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“…Glucosidases I and II sequentially remove specific glucosyl residues from glycan precursors attached to proteins as part of the trimming that occurs during the synthesis of membrane and secreted proteins. Mutational analyses show that both glucosidases are essential for normal embryo development in Arabidopsis (Arabidopsis thaliana; Boisson et al, 2001;Burn et al, 2002;Gillmor et al, 2002;Furumizu and Komeda, 2008;Soussillane et al, 2009). In conditional mutants that can germinate under permissive conditions, loss of glucosidase II from seedlings causes dramatic reduction in root elongation (Burn et al, 2002;Soussillane et al, 2009).…”

Section: Some Maltase Inhibitors Also Affect Other Unknown Proteins mentioning

confidence: 99%

“…Mutational analyses show that both glucosidases are essential for normal embryo development in Arabidopsis (Arabidopsis thaliana; Boisson et al, 2001;Burn et al, 2002;Gillmor et al, 2002;Furumizu and Komeda, 2008;Soussillane et al, 2009). In conditional mutants that can germinate under permissive conditions, loss of glucosidase II from seedlings causes dramatic reduction in root elongation (Burn et al, 2002;Soussillane et al, 2009). Similarly, a weak mutant allele of the gene encoding glucosidase I permits germination but subsequent root growth is retarded (Furumizu and Komeda, 2008).…”

Section: Some Maltase Inhibitors Also Affect Other Unknown Proteins mentioning

confidence: 99%

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The Role of α-Glucosidase in Germinating Barley Grains

Stanley

Rejzek

Næsted³

et al. 2010

Plant Physiology

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The importance of a-glucosidase in the endosperm starch metabolism of barley (Hordeum vulgare) seedlings is poorly understood. The enzyme converts maltose to glucose (Glc), but in vitro studies indicate that it can also attack starch granules. To discover its role in vivo, we took complementary chemical-genetic and reverse-genetic approaches. We identified iminosugar inhibitors of a recombinant form of an a-glucosidase previously discovered in barley endosperm (ALPHA-GLUCOSIDASE97 [HvAGL97]), and applied four of them to germinating grains. All four decreased the Glc-to-maltose ratio in the endosperm 10 d after imbibition, implying inhibition of maltase activity. Three of the four inhibitors also reduced starch degradation and seedling growth, but the fourth did not affect these parameters. Inhibition of starch degradation was apparently not due to inhibition of amylases. Inhibition of seedling growth was primarily a direct effect of the inhibitors on roots and coleoptiles rather than an indirect effect of the inhibition of endosperm metabolism. It may reflect inhibition of glycoprotein-processing glucosidases in these organs. In transgenic seedlings carrying an RNA interference silencing cassette for HvAgl97, a-glucosidase activity was reduced by up to 50%. There was a large decrease in the Glc-to-maltose ratio in these lines but no effect on starch degradation or seedling growth. Our results suggest that the a-glucosidase HvAGL97 is the major endosperm enzyme catalyzing the conversion of maltose to Glc but is not required for starch degradation. However, the effects of three glucosidase inhibitors on starch degradation in the endosperm indicate the existence of unidentified glucosidase(s) required for this process.

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Section: Some Maltase Inhibitors Also Affect Other Unknown Proteins mentioning

confidence: 99%

Section: Some Maltase Inhibitors Also Affect Other Unknown Proteins mentioning

confidence: 99%

The Role of α-Glucosidase in Germinating Barley Grains

Stanley

Rejzek

Næsted³

et al. 2010

Plant Physiology

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“…The samples were evaporated to dryness, washed with distilled water, dried again in a SpeedVac manifold and suspended in 25 mL of 25 mM NH 4 Ac, pH 5. The addition of peptide:N-glycanase A (PNGase A, Roche Diagnostics, Mannheim, Germany) resulted in the release of the N-glycans from the glycopeptides.…”

Section: Glycan Release From Peptidesmentioning

confidence: 99%

“…The dried glycopeptides were resolved in 20 mL of 25 mM NH 4 Ac, pH 5, and incubated at 951C for 6 min. The samples were cooled to RT and centrifuged briefly.…”

Section: Glycan Release From Peptidesmentioning

confidence: 99%

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Improved sample preparation for CE‐LIF analysis of plant N‐glycans

et al. 2011

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In view of glycomics studies in plants, it is important to have sensitive tools that allow one to analyze and characterize the N-glycans present on plant proteins in different species. Earlier methods combined plant-based sample preparations with CE-LIF N-glycan analysis but suffered from background contaminations, often resulting in non-reproducible results. This publication describes a reproducible and sensitive protocol for the preparation and analysis of plant N-glycans, based on a combination of the 'in-gel release method' and N-glycan analysis on a multicapillary DNA sequencer. Our protocol makes it possible to analyze plant N-glycans starting from low amounts of plant material with highly reproducible results. The developed protocol was validated for different plant species and plant cells.

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Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2009–2010

Harvey

2014

Mass Spectrometry Reviews

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This review is the sixth update of the original article published in 1999 on the application of MALDI mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2010. General aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, arrays and fragmentation are covered in the first part of the review and applications to various structural typed constitutes the remainder. The main groups of compound that are discussed in this section are oligo and polysaccharides, glycoproteins, glycolipids, glycosides and biopharmaceuticals. Many of these applications are presented in tabular form. Also discussed are medical and industrial applications of the technique, studies of enzyme reactions and applications to chemical synthesis.

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N-glycan trimming by glucosidase II is essential for Arabidopsis development

Cited by 39 publications

References 50 publications

The Role of α-Glucosidase in Germinating Barley Grains

The Role of α-Glucosidase in Germinating Barley Grains

Improved sample preparation for CE‐LIF analysis of plant N‐glycans

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2009–2010

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