N-Glycosylation during translation is essential for human arylacetamide deacetylase enzyme activity

Muta, Kyotaka; Fukami, Tatsuki; Nakajima, Miki; Yokoi, Tsuyoshi

doi:10.1016/j.bcp.2013.10.001

Cited by 11 publications

(12 citation statements)

References 35 publications

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“…However, the glycosylation seemed to affect the activity of the enzymes, and the enzymatic activity of the recombinant proteins was decreased after deglycosylation (Fig 3). This finding is consistent with previous studies showing that glycosylation is required for the full activity of a wide variety of enzymes [31, 32]. The influence of glycosylation on protein properties may be attributed to several functions such as ensuring correct protein folding, preventing proteolytic degradation, and facilitating intracellular transportation [31].…”

Section: Resultssupporting

confidence: 92%

“…This finding is consistent with previous studies showing that glycosylation is required for the full activity of a wide variety of enzymes [31, 32]. The influence of glycosylation on protein properties may be attributed to several functions such as ensuring correct protein folding, preventing proteolytic degradation, and facilitating intracellular transportation [31]. Moreover, glycosylation is one of the greatest advantages of the yeast expression systems [25, 26].…”

Section: Resultssupporting

confidence: 91%

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Design, expression and functional characterization of a thermostable xylanase from Trichoderma reesei

Tang

Chen

et al. 2019

PLoS ONE

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Xylanases isolated from microorganisms such as the Trichoderma reesei have attracted considerable research interest because of their potential in various industrial applications. However, naturally isolated xylanases cannot withstand harsh conditions such as high temperature and basic pH. In this study, we performed structural analysis of the major T. reesei xylanase (Xyn2), and novel flexible regions of the enzyme were identified based on B-factor, a molecular dynamics (MD) parameter. To improve thermostability of the Xyn2, disulfide bonds were introduced into the unstable flexible region by using site-directed mutagenesis and two recombinant xylanases, XM1 (Xyn2Cys12-52) and XM2 (Xyn2Cys59-149) were successfully expressed in Pichia pastoris. Secreted recombinant Xyn2 was estimated by SDS-PAGE to be 24 kDa. Interestingly, the half-lives of XM1 and XM2 at 60°C were 2.5- and 1.8- fold higher, respectively than those of native Xyn2. The XM1 also exhibited improved pH stability and maintained more than 60% activity over pH values ranging from 2.0 to 10.0. However, the specific activity and catalytic efficiency of XM1 was decreased as compared to those of XM2 and native Xyn2. Our results will assist not only in elucidating of the interactions between protein structure and function, but also in rational target selection for improving the thermostability of enzymes.

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Section: Resultssupporting

confidence: 92%

Section: Resultssupporting

confidence: 91%

Design, expression and functional characterization of a thermostable xylanase from Trichoderma reesei

Tang

Chen

et al. 2019

PLoS ONE

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“…Univariable Cox regression analysis, lasso-penalized Cox regression analysis, and multivariable Cox analysis suggested that five SRGs [AADAC (P = 0.008), DEF8 (P = 0.026), HIST1H1C(P = 0.031, MET(P = 0.001), and CHFR(P = 0.041)] were significantly associated with the prognosis of resectable PC,. The arylacetamide deacetylase gene (AADAC) lies on chromosome 3q25.1, and its expressed protein consists of 399 amino acids [34,35]. The protein coded by AADAC protein is extensively implicated in the hydrolysis of various drugs [36], whose function may be related to chemotherapy resistance in pancreatic cancer.…”

Section: Discussionmentioning

confidence: 99%

Five gene signatures were identified in the prediction of overall survival in resectable pancreatic cancer

Tian

2020

BMC Surg

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Background Although genes have been previously detected in pancreatic cancer (PC), aberrant genes that play roles in resectable pancreatic cancer should be further assessed. Methods Messenger RNA samples and clinicopathological data corrected with PC were downloaded from The Cancer Genome Atlas (TCGA). Resectable PC patients were randomly divided into a primary set and a validation set. Univariable Cox regression analysis, lasso-penalized Cox regression analysis, and multivariable Cox analysis were implemented to distinguish survival-related genes (SRGs). A risk score based on the SRGs was calculated by univariable Cox regression analysis. A genomic-clinical nomogram was established by integrating the risk score and clinicopathological data to predict overall survival (OS) in resectable PC. Results Five survival-related genes (AADAC, DEF8, HIST1H1C, MET, and CHFR) were significantly correlated with OS in resectable PC. The resectable PC patients, based on risk score, were sorted into a high-risk group that showed considerably unfavorable OS (p < 0.001) than the low-risk group, in both the primary set and the validation set. The concordance index (C-index) was calculated to evaluate the predictive performance of the nomogram were respectively in the primary set [0.696 (0.608–0.784)] and the validation set [0.682 (0.606–0.758)]. Additionally, gene set enrichment Analysis discovered several meaningful enriched pathways. Conclusion Our study identified five prognostic gene biomarkers for OS prediction and which facilitate postoperative molecular target therapy for the resectable PC, especially the nomic-clinical nomogram which may be used as an effective model for the postoperative OS evaluation and also an optimal therapeutic tool for the resectable PC.

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“…Similar promotional effects were also observed in several related biocatalysts that modified GlcNAc-6-phosphate to GlcN-6-Phosphate. Anderson and his colleagues used the method to make glucosamine fungal as a microbial biomass resource (Scheme 4) [63].…”

Section: Biocatalytic Potential Of Enzymes Involved the Modification Of Glucosamine In Chemotherapeuticalmentioning

confidence: 99%

Biocatalytic Potential of Enzymes Involved the Modification of Glucosamine in Chemotherapeutical Intermediates Synthesis

Senan¹

2021

J Food Chem Nanotechnol

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The enzymatic modification of glucosamine (GlcN) and its derivatives have a keen interest for researchers due to the natural abundance of its bio-sourced such as chitin. The recent progress for understanding the role of glucosamine based on various novel approaches for enzymatic synthesis in the field of medicinal chemistry and food supplements. These developments led to improve chemo-selective protection and/or deprotection of amino-sugar during chainenhancement and allow the simultaneous regioselective reaction of functionally relevant acetyl, phosphate, or sulfate groups. In this review, we give an overview of current strategies on the synthesis of therapeutically relevant glucosaminecontaining derivatives.

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N-Glycosylation during translation is essential for human arylacetamide deacetylase enzyme activity

Cited by 11 publications

References 35 publications

Design, expression and functional characterization of a thermostable xylanase from Trichoderma reesei

Design, expression and functional characterization of a thermostable xylanase from Trichoderma reesei

Five gene signatures were identified in the prediction of overall survival in resectable pancreatic cancer

Biocatalytic Potential of Enzymes Involved the Modification of Glucosamine in Chemotherapeutical Intermediates Synthesis

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