N-glycosylation Site Analysis Reveals Sex-related Differences in Protein N-glycosylation in the Rice Brown Planthopper (Nilaparvata lugens)

Abstract

Glycosylation is a common modification of proteins and critical for a wide range of biological processes. Differences in protein glycosylation between sexes have already been observed in humans, nematodes and trematodes, and have recently also been reported in the rice pest insect Nilaparvata lugens. Although protein N-glycosylation in insects is nowadays of high interest because of its potential for exploitation in pest control strategies, the functionality of differential N-glycosylation between sexes is yet unknown. In this study, therefore, the occurrence and role of sex-related protein N-glycosylation in insects were examined. A comprehensive investigation of the N-glycosylation sites from the adult stages of N. lugens was conducted, allowing a qualitative and quantitative comparison between sexes at the glycopeptide level. N-glycopeptide enrichment via lectin capturing using the high mannose/paucimannose-binding lectin Concanavalin A, or the Rhizoctonia solani agglutinin which interacts with complex N-glycans, resulted in the identification of over 1300 N-glycosylation sites derived from over 600 glycoproteins. Comparison of these N-glycopeptides revealed striking differences in protein N-glycosylation between sexes. Male- and female-specific N-glycosylation sites were identified, and some of these sex-specific N-glycosylation sites were shown to be derived from proteins with a putative role in insect reproduction. In addition, differential glycan composition between males and females was observed for proteins shared across sexes. Both lectin blotting experiments as well as transcript expression analyses with complete insects and insect tissues confirmed the observed differences in N-glycosylation of proteins between sexes. In conclusion, this study provides further evidence for protein N-glycosylation to be sex-related in insects. Furthermore, original data on N-glycosylation sites of N. lugens adults are presented, providing novel insights into planthopper's biology and information for future biological pest control strategies.

Improved methanol tolerance of Rhizomucor miehei lipase based on N‑glycosylation within the α-helix region and its application in biodiesel production

“…Therefore, improving the catalytic activity, methanol tolerance, and transesterification efficiency of lipase has attracted widespread attention [ 29 ]. At present, structure-based semi-rational design is an effective strategy for improving enzyme performance, which is undertaken by adding modifications to particular positions along the enzyme [ 30 , 31 ].…”

35 years in plant lectin research: a journey from basic science to applications in agriculture and medicine

“…The binding of plant lectins to glycosylated proteins of insects being an important interaction to explain the insecticidal activity of lectins, prompted us to study in more detail the glycosylation in insects and the importance of glycosylation for insect development. Analyses of the N-glycomes from several insects revealed that high-mannose and paucimannose N-glycans are highly abundant, explaining why especially mannose-binding lectins show good interaction with insect glycoconjugates [109][110][111][112]. Recent research also indicated differences in the N-glycan profile for male and female insects of Nilparvata lugens, with male insects containing less high-mannose and more paucimannose type structures compared to the female insects [110,111].…”

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“…Hydrophilic enrichment followed by high mass accuracy MS employing complementary fragmentation techniques (higher‐energy collision dissociation and electron transfer dissociation) proved to be an effective method for N‐glycopeptide identification (Zeng et al, 2018). The study of N‐linked glycosylation was recently enhanced using a newly developed method that elegantly combines filter‐aided sample preparation with lectin affinity chromatography (Scheys et al, 2020). Thousands of N‐GlcNAc modified sites have been identified using these enrichment methods, and the functions of these modifications have been interpreted (Bi et al, 2021).…”