N-halamine surface coating for mitigation of biofilm and microbial contamination in water systems for space travel

Demir, Buket; Taylor, Alex H.; Broughton, R. M.; Huang, Tung‐Shi; Bozack, Michael J.; Worley, S. D.

doi:10.1016/j.bioflm.2022.100076

Cited by 8 publications

(12 citation statements)

References 30 publications

(43 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is established that the surface concentration of oxidative chlorine as low as 5 × 10 15 atoms/ cm 2 is sufficient to provide an antimicrobial effect. 28 Therefore, it is evident that our polymer-coated and halogenated fabrics possessed large excess of oxidative halogen for the "contact killing", even considering that only a fraction of that halogen would be available for contact with the microorganism at the fibrous fabric/water interface. To estimate the release of halogen from the polymer-coated, halogenated fabrics via the > N−X bond hydrolysis and dissociation, time-dependent quantitative evaluation of the oxidative and total halogen contents in the immersing solutions was conducted at the initial stage (up to 1 h), and total halogen contents were measured at 1 and 28 days since the release commencement (Figure 5).…”

Section: Effects Of Halogenation 331 Halogen Releasementioning

confidence: 90%

Nonleaching Biocidal N-Halamine-Functionalized Polyamine-, Guanidine-, and Hydantoin-Based Coatings

Bromberg,

Magariños,

Concheiro

et al. 2024

Ind. Eng. Chem. Res.

View full text Add to dashboard Cite

Fibrous materials with inherent antimicrobial properties can help in real-time deactivation of microorganisms, enabling multiple uses while reducing secondary infections. Coatings with antiviral polymers enhance the surface functionality for existing and potential future pandemics. Herein, we demonstrated a straightforward route toward biocidal surface creation using polymers with nucleophilic biguanide, guanidine, and hydantoin groups that are covalently attached onto a solid support. Biocidal poly(N-vinylguanidine) (PVG) and poly(allylamine-co-4-aminopyridine-co-5-(4-hydroxybenzylidene)hydantoin) (PAH) were introduced for coating applications along with commercially available polyvinylamine (PVAm) and poly(hexamethylene biguanide) (PHMB). Nonleaching coatings were created by first fabricating bifunctional siloxane or isocyanate precursor coatings on the cotton, nylon–cotton, and glass fiber fabric, followed by the polymer attachment. The developed grafting methods ensured the stability of the coating and the reuse of the material while maintaining the biocidal properties. Halogenation of polymer-coated fabric was conducted by aqueous solutions of sodium hypochlorite or in situ generation of hypobromous acid (HOBr), resulting in surfaces coated by N-halamines with high contents of active > N–Cl or > N–Br groups. The polymer-coated fabrics were stable in multiple laundry cycles and maintained hydrophilic character after coating and halogenation. Halogenated polymer-coated fabrics completely inactivated human respiratory coronavirus based on a contact-killing mechanism and were shown to be reusable after recharging with bromine or chlorine.

show abstract

Section: Effects Of Halogenation 331 Halogen Releasementioning

confidence: 90%

Nonleaching Biocidal N-Halamine-Functionalized Polyamine-, Guanidine-, and Hydantoin-Based Coatings

Bromberg,

Magariños,

Concheiro

et al. 2024

Ind. Eng. Chem. Res.

View full text Add to dashboard Cite

show abstract

“…Biocidal kinetics of cotton swatches decorated with different functionalities against Gram‐positive S. aureus and Gram‐positive E. coli was measured via Sandwich test method 7 using pristine ones as controls for the analysis of the combination effect. Typically, both strains were collected by centrifugation after cultivation in broth solution at 250 rpm and 37°C for 24 h. Then, the collected strains were rinsed twice with a phosphate‐buffered saline (PBS) solution and diluted with PBS to a suspension of a concentration of 10 8 –10 9 CFU/mL.…”

Section: Methodsmentioning

confidence: 99%

“…5,6 The representatives of organic ones are N-halamines (N Cl, N Br, etc. ), [7][8][9][10] cationic salts (quaternary ammonium salts, quaternary phosphonium salts, etc. ), [11][12][13][14] aldehydes, alcohols and phenols, and biguanides.…”

Section: Introductionmentioning

confidence: 99%

Construction of silicone with quaternized hydantoin‐based N‐chloramines on cotton with aid of supercritical interpenetration for antibacterial synergism

Chen,

Suo,

Pei

et al. 2024

J of Applied Polymer Sci

View full text Add to dashboard Cite

A silicone carrying combined functionalities of different mechanisms and physiochemical properties is synthesized as a strategy for faster and broader bacterial eliminations. Briefly, ring‐opening alcoholysis of succinic anhydride with 2‐dimethylaminoethanol and subsequent esterification of the alcoholysis product with 5‐(4‐hydroxyphenyl)imidazolidine‐2,4‐dione produced a structure with one tertiary amine and two hydantoinyl NH bonds. The tertiary amine was quaternized with 3‐chloro‐1‐propanol whose OH was then alcoholyzed with SiH of poly(methylhydrosiloxane). The polymeric product was interpenetrated into cotton at 30 MPa and 50°C in supercritical CO2 and treated with tert‐butyl hypochlorite to chlorinate its hydantoinyl NH bonds, forming a 78 nm biocidal layer of silicone with quaternized hydantoin‐based N‐chloramines. Antibacterial tests showed that the quaternary ammonium salt and two hydantoin‐based N‐chloramines act synergistically due to their complementary features, killing both Staphylococcus aureus and Escherichia coli much more efficiently than each functionality. More importantly, the ratio of N‐chloramine to quaternary ammonium salt of 2:1 herein also provided higher efficacy than a reported counterpart with a 1:1 ratio. Biocidability of the modified cotton had promising stability and rechargeability, which indicates that supercritical interpenetration produced a durable interfacial layer without the necessity of chemical bonding.

show abstract

“…A 50 cm × 5 cm stainless steel (Inconel 625, HPALLOY, IN) metal plate was placed inside the reactor as the surface for biofilm formation. Inconel stainless steel is widely used in water systems (i.e., tanks, piping, cooling towers) and has been previously proven to accumulate biofilm rapidly. , …”

Section: Methodsmentioning

confidence: 99%

“…Inconel stainless steel is widely used in water systems (i.e., tanks, piping, cooling towers) and has been previously proven to accumulate biofilm rapidly. 21,22 A single 50 cm custom-made quartz SEOF was inserted on the top of the Inconel surface in the same way as the apparatus reported in previous study. 23 A fixed spacing (∼0.3 cm) was intentionally maintained between SEOF and the Inconel surface, and therefore any grown biofilms were perpendicular to the UV-C light side emitted from the SEOF (Figure SI.2).…”

Section: Microbial Culture Preparationmentioning

confidence: 99%

Phenotypic and Transcriptional Responses of Pseudomonas aeruginosa Biofilms to UV-C Irradiation via Side-Emitting Optical Fibers: Implications for Biofouling Control

Zhao,

Luo,

Wang

et al. 2023

Environ. Sci. Technol.

View full text Add to dashboard Cite

Biofilms give rise to a range of issues, spanning from harboring pathogens to accelerating microbial-induced corrosion in pressurized water systems. Introducing germicidal UV-C (200−280 nm) irradiation from light-emitting diodes (LEDs) into flexible side-emitting optical fibers (SEOFs) presents a novel light delivery method to inhibit the accumulation of biofilms on surfaces found in small-diameter tubing or other intricate geometries. This work used surfaces fully submerged in flowing water that contained Pseudomonas aeruginosa, an opportunistic pathogen commonly found in water system biofilms. A SEOF delivered a UV-C gradient to the surface for biofilm inhibition. Biofilm growth over time was monitored in situ using optical conference tomography. Biofilm formation was effectively inhibited when the 275 nm UV-C irradiance was ≥8 μW/cm 2 . Biofilm samples were collected from several regions on the surface, representing low and high UV-C irradiance. RNA sequencing of these samples revealed that high UV-C irradiance inhibited the expression of functional genes related to energy metabolism, DNA repair, quorum sensing, polysaccharide production, and mobility. However, insufficient sublethal UV-C exposure led to upregulation genes for SOS response and quorum sensing as survival strategies against the UV-C stress. These results underscore the need to maintain minimum UV-C exposure on surfaces to effectively inhibit biofilm formation in water systems.

show abstract

N-halamine surface coating for mitigation of biofilm and microbial contamination in water systems for space travel

Cited by 8 publications

References 30 publications

Nonleaching Biocidal N-Halamine-Functionalized Polyamine-, Guanidine-, and Hydantoin-Based Coatings

Nonleaching Biocidal N-Halamine-Functionalized Polyamine-, Guanidine-, and Hydantoin-Based Coatings

Construction of silicone with quaternized hydantoin‐based N‐chloramines on cotton with aid of supercritical interpenetration for antibacterial synergism

Phenotypic and Transcriptional Responses of Pseudomonas aeruginosa Biofilms to UV-C Irradiation via Side-Emitting Optical Fibers: Implications for Biofouling Control

Contact Info

Product

Resources

About