N-Myc and Bcl-2 coexpression induces MMP-2 secretion and activation in human neuroblastoma cells

Noujaim, Daniel L.; Golen, Cynthia M. van; Golen, Kenneth L. van; Grauman, Alyssa; Feldman, Eva L.

doi:10.1038/sj.onc.1205552

Cited by 47 publications

(38 citation statements)

References 54 publications

(73 reference statements)

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“…Bcl2 cooperation with c-Myc increases the expression, secretion, and activation of MMP-2, resulting in cell invasion [29]. For further analysis whether hypoxia induces Bcl2 expression, Western blot analysis showed that hypoxia significantly increased Bcl2 expression.…”

Section: Discussionmentioning

confidence: 98%

c-Myc is essential for urokinase plasminogen activator expression on hypoxia-induced vascular smooth muscle cells

Hou¹,

Okamoto²,

Okada³

et al. 2007

Cardiovascular Research

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Section: Discussionmentioning

confidence: 98%

c-Myc is essential for urokinase plasminogen activator expression on hypoxia-induced vascular smooth muscle cells

Hou¹,

Okamoto²,

Okada³

et al. 2007

Cardiovascular Research

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“…This pathway is able to activate the MMP-9 promoter, explaining the increased invasive potential of cells expressing Bcl-2. It was also shown, in neuroblastoma cell lines, that coexpression of NMyc and Bcl-2 leads to increased tumorigenic properties (Noujaim et al, 2002). MMP-2 expression and secretion were increased in cells expressing Bcl-2 alone, or both N-Myc and Bcl-2.…”

Section: A Brief Note On Transformation and Growth As Related To The mentioning

confidence: 90%

The "chemoinvasion assay": a tool to study tumor and endothelial cell invasion of basement membranes

Albini¹,

Benelli²,

Noonan³

et al. 2004

Int. J. Dev. Biol.

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Several genetic and epigenetic factors, both in the cell and in the host, contribute to the progression of tumors towards metastases. The escape of cancer cells from a primary, localized tumor to distant organs transforms a relatively curable pathology to an almost untreatable one. Metastatic lesions are often resistant to cancer therapy because of the progressive phenotypic changes that they have undergone. In this article we will give a bird's eye view on the features of metastatic cells and potential therapeutic targets. In particular, the invasion of basement membranes represents a fundamental step for cell dispersion. Over seventeen years ago we established the Matrigel "chemoinvasion" assay, a useful tool for studying the mechanisms involved in tumor and endothelial cell invasion of basement membranes and for the screening of anti-invasive agents. We will describe the assay and review some of the major results it enabled to obtain.

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“…Cells were rinsed, resuspended in DMEM, and 6 Â 10 4 cells in 100 AL DMEM were plated on top of the HBME cells. After 48 hours, nonmigrating cells were removed from the upper surface of the membrane, whereas migrating cells on the bottom surface were stained, eluted, and quantitated as previously described (11). Ten percent calf serum in DMEM was used as a positive control chemoattractant; DMEM only was used as a negative control.…”

Section: Methodsmentioning

confidence: 99%

“…IMR32 cells were plated on top of the HBME cells or directly onto the transwell surface in either DMEM or DMEM + 0.3 Am NVP. Cells were allowed to migrate for 48 hours, then nonmigrating cells were removed from the upper surface of the membrane, whereas migrating cells on the bottom surface were stained, eluted, and quantitated as previously described (11). Columns, average of three experiments; bars, SE.…”

Section: Igf-ir-expressing Neuroblastoma Cells Dock At Hbme Cellsmentioning

confidence: 99%

“…IGF-IR promotes neuroblastoma motility (9), migration (10), and invasion (11), steps necessary for dissemination of tumor cells. The ''seed and soil'' hypothesis of metastasis, developed by Steven Paget, states that the molecular profile of a tumor cell (seed) must match the molecular profile of the organ (soil) for secondary tumor formation (12).…”

Section: Introductionmentioning

confidence: 99%

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Insulin-Like Growth Factor-I Receptor Expression Regulates Neuroblastoma Metastasis to Bone

et al. 2006

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Neuroblastoma is a pediatric tumor that preferentially metastasizes to bone. Patients with bone metastases have a mortality rate >93%, indicating a need for novel treatment targets. Our laboratory has shown that type I insulin-like growth factor receptor (IGF-IR) expression and activation regulate neuroblastoma cell proliferation, motility, invasion, and survival, and that expression of the IGF-IR correlates with neuroblastoma tumorigenicity. Bone expresses large amounts of IGF ligands, and the IGF system is required for normal bone physiology. The current study addresses the role of the IGF system in neuroblastoma metastasis to bone. Upon reaching the bone marrow through the circulation, neuroblastoma cells must dock at the bone marrow endothelium, extravasate into the bone microenvironment, and destroy bone tissue to allow for tumor growth. This report examines the effects of high IGF-IR expression on neuroblastoma cell interaction with bone. The current data show that neuroblastoma cells with high IGF-IR expression, either endogenously or through transfection, adhere to human bone marrow endothelial cells and subsequently migrate toward both IGF-I and human bone stromal cells. High IGF-IR-expressing neuroblastoma cells adhere tightly to bone stromal cells, flatten, and extend processes. When neuroblastoma cells are injected directly into the tibiae of mice, those cells with increased IGF-IR form both osteolytic lesions within the tibiae and secondary tumors within other sites. These results support the hypothesis that IGF-IR expression in neuroblastoma cells increases tumor cell interaction with the bone microenvironment, resulting in greater formation of metastases. (Cancer Res 2006; 66(13): 6570-8)

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N-Myc and Bcl-2 coexpression induces MMP-2 secretion and activation in human neuroblastoma cells

Cited by 47 publications

References 54 publications

c-Myc is essential for urokinase plasminogen activator expression on hypoxia-induced vascular smooth muscle cells

c-Myc is essential for urokinase plasminogen activator expression on hypoxia-induced vascular smooth muscle cells

The "chemoinvasion assay": a tool to study tumor and endothelial cell invasion of basement membranes

Insulin-Like Growth Factor-I Receptor Expression Regulates Neuroblastoma Metastasis to Bone

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