N-Oleoyl-Phosphatidyl-Ethanolamine and Epigallo Catechin-3-Gallate Mitigate Oxidative Stress in Overweight and Class I Obese People on a Low-Calorie Diet

Abstract

Oxidative stress and lipid peroxidation are considered key factors linking obesity with its associated complications. Epigallo catechin-3-gallate (EGCG) and oleoylethanolamide, together with its phospholipid precursor N-oleoylphosphatidylethanolamine (NOPE), are nutritional compounds that might improve the oxidative stress status of obese people. Unfortunately, the bioavailability of these compounds is low; however, the coadministration of NOPE with EGCG has been shown to ameliorate both the plasma availability of EGCG and the intestinal levels of NOPE in rats. This double-blind placebo-controlled study investigated the effects of 2 months' supplementation with EGCG complexed with NOPE, combined with moderate energy restriction, on plasma oxidative status of overweight and class I obese subjects. A total of 138 subjects (body mass index: 25-35 kg/m 2 ) were recruited and randomized into two groups: the first (n = 67) received caps of placebo and the second (n = 71) caps of an oily dispersion of EGCG complexed with NOPE for 2 months. Subjects' supplementation was combined with moderate energy restriction (-800 kcal/day). Plasma oxidative status was determined by measuring the levels of oxidized low-density lipoprotein (Ox-LDL), malondialdehyde and reactive oxygen metabolites, and by calculating the lag time and the slope of Cu-induced lipid peroxidation kinetics. In total 116 subjects (27 M/89 F) completed the supplementation period, 49 in the placebo group and 67 in the treated group. Treatment induced a similar significant weight reduction in the two groups. Moreover, we found the mean changes of Ox-LDL significantly lower and the mean changes of antioxidant capacity (lag time) significantly higher in NOPE-EGCG group than in placebo group (treatment effect mean difference: -3.15 UL, P < .044 and +5.37 min, P < .0347, respectively). EGCG plasma levels were detectable only after 2 months of NOPE-EGCG diet. The NOPE-EGCG integration to a low-energy diet seems, therefore, useful for ameliorating oxidative stressrelated markers, which are concomitant causes of obesity-induced disorders.

Effect of Green Tea Supplementation on Antioxidant Status in Adults: A Systematic Review and Meta-Analysis of Randomized Clinical Trials

“…After screening of title and abstract, 2288 unrelated studies were discarded due to primary evaluation of inclusion criteria: unrelated title ( n = 1729), review ( n = 123), and animal study (436). Consequently, 42 studies were retrieved for full-text review, 26 of which were excluded due to: lack of sufficient data and the use of two or more interventions at the same time [ 2 , 21 , 22 , 24 , 30 , 34 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 ]. Finally, sixteen RCTs with one double-arm study [ 4 , 5 , 9 , 10 , 13 , 22 , 24 , 29 , 37 ] were eligible for this systematic review and meta-analysis.…”

Multiple Micronutrients and Docosahexaenoic Acid Supplementation during Pregnancy: A Randomized Controlled Study

“…The omega-3 index was calculated by summing the percentage of EPA and DHA [43]. Calcidiol levels were measured using radioimmunoassay [44], the GSH/GSSG ratio using fluorimetric assay [1], ROMs using photometric assay [45,46], and 8-isoprostane using competitive enzyme immunoassay with an ELISA kit (Cayman Chemical, Ann Arbor, MI, USA) following the manufacturer's instructions [47]. Briefly, 500 uL of heparinated plasma were stored at -80 • C with a preservative ethanol solution containing butylated hydroxytoluene (BHT) until analysis.…”

Dietary adaptation for weight loss maintenance at Yale (DAWLY): Protocol and predictions for a randomized controlled trial

“…Specifically, we have designed DAWLY, an RCT to test if habitual fat intake moderates the effect of OEA on weight loss and weight loss maintenance in the context of a well-established longstanding evidence-based behavioral weight loss program (LEARN ® ) ( 58 ). Prior studies using supplementation with OEA to improve weight loss have been met with limited success ( 75 ). Since HFD depletes OEA and acute administration of OEA can reverse the effects of HFD on vagal afferent induction of DA release, we reasoned that OEA supplementation might be most beneficial for individuals who consume a HFD.…”