N-recognins UBR1 and UBR2 as central ER stress sensors in mammals

Le, Ly Thi Huong Luu; Park, Seoyoung; Lee, Jung Hoon; Kim, Yun Kyung; Lee, Min Jae

doi:10.1016/j.mocell.2023.12.001

Molecules and Cells

2024

DOI: 10.1016/j.mocell.2023.12.001

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N-recognins UBR1 and UBR2 as central ER stress sensors in mammals

Ly Thi Huong Luu Le,

Seoyoung Park,

Jung Hoon Lee

et al.

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2024

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Development of an enhanced anti-pan-N-formylmethionine-specific antibody

Kim,

Park,

Hwang

2024

Preprint

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Both bacterial and eukaryotic ribosomes can initiate protein synthesis with formylmethionine (fMet), but detecting fMet-bearing peptides and fMet-bearing proteins has been challenging due to the lack of effective anti-pan-fMet antibodies. Previously, we developed a polyclonal anti-fMet antibody using a fMet-Gly-Ser-Gly-Cys pentapeptide that detects those fMet-bearing peptides and fMet-bearing proteins regardless of their sequence context. In this study, we significantly improved the antibody's specificity and affinity by using a mixture of fMet-Xaa-Cys (fMXC) tripeptides (Xaa, any of the 20 amino acids) as the immunogen. This newly optimized anti-fMet antibody is a powerful, cost-effective tool for detecting fMet-bearing proteins across species. Furthermore, this approach provides a foundation for developing anti-pan-specific antibodies targeting other N-terminal modifications through acylation, alkylation, oxidation, or arginylation, etc.

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Development of an enhanced anti-pan-N-formylmethionine-specific antibody

Kim,

Park,

Hwang

2024

Preprint

View full text Add to dashboard Cite

show abstract

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N-recognins UBR1 and UBR2 as central ER stress sensors in mammals

Cited by 1 publication

References 30 publications

Development of an enhanced anti-pan-N-formylmethionine-specific antibody

Development of an enhanced anti-pan-N-formylmethionine-specific antibody

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