N<sub>2</sub> FIXATION AND COMPETITIVENESS OF <i>Rhizobium phaseoli</i> STRAINS ISOLATED FROM ONTARIO SOILS

Moxley, Jacqueline C.; Hume, D. J.; Smith, Donald L.

doi:10.4141/cjps86-104

Cited by 21 publications

(7 citation statements)

References 17 publications

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“…Two effective strains did not elicit significant increases in shoot and pod dry matter yields of field-grown beans relative to uninoculated plants, indicating that the soils already contained efficient strains. Similar results were obtained in Ontario where efficient isolates obtained from 37 farm sites elicited seed yields on Phaseolus vulgaris that did not differ significantly from plants inoculated with efficient strains taken from culture collections (Moxley et al 1986). …”

Section: Rhizobium Leguminosarumsupporting

confidence: 73%

Diversity of symbiotic rhizobia resident in Canadian soils

Bromfield¹

2003

Can. J. Soil. Sci.

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. 2003. Diversity of symbiotic rhizobia resident in Canadian soils. Can. J. Soil Sci. 83: 311-319. The dependency of agriculture on nitrogen fertilizer inputs is associated with adverse effects on the environment and human health. The importance of biological nitrogen fixation by rhizobia in symbiotic association with legumes is underscored by its potential to reduce or replace chemical fertilizer inputs. This paper reviews research on the diversity of the symbiotic rhizobia resident in Canadian soils. Research has focussed on phenotypic and genotypic variation (e.g., nitrogen fixing efficacy, nodulating competitiveness, host range, adaptation to cool climate) within rhizobial species with the objective of selecting efficient strains for use in inoculants for legume crops. The genetic diversity of rhizobia resident in Canadian soils has been reported only for Sinorhizobium meliloti, Rhizobium leguminosarum and Mesorhizobium spp. There is a need for further studies on populations of other rhizobial species, particularly those associated with native legumes. Exploiting the diversity present in natural soil populations via selection and genetic manipulation should permit the development of superior strains for use in legume inoculants. Other rhizobial traits that may be exploited include plant growth-promoting activity and ability to degrade pollutants. 311-319. La dépendance de l'agriculture sur les engrais azotés est souvent associée à des répercussions néfastes au niveau de la santé humaine et de la qualité de l'environnement. Parallèlement, on sous-estime l'importance de la fixation de l'azote biologique par les rhizobiums en symbiose avec les légumineuses, phénomène qui pourrait nous aider à réduire l'emploi des engrais chimiques ou à remplacer ceux-ci. Cet article passe en revue la recherche sur les rhizobiums symbiotiques présents dans les sols canadiens. Les travaux se concentrent sur la variation du phénotype et du génotype (à savoir, efficacité de la fixation de l'azote, compétitivité au niveau des nodules, gamme d'hôtes, adaptation au climat frais) des diverses espèces, cela dans le but de sélec-tionner les meilleures souches, dont on se servira comme inoculant pour les légumineuses. Dans les sols canadiens, on ne s'est attardé qu'à la diversité génétique de Sinorhizobium meliloti, de Rhizobium leguminosarum et des espèces du genre Mesorhizobium. Il conviendrait d'étudier la population d'autres rhizobiums, en particulier ceux associés aux légumineuses indigènes. En tirant parti de la diversité observée dans les populations naturelles, par sélection ou par manipulation génétique, on devrait obtenir des souches de meilleure qualité pour l'inoculation. D'autres caractères des rhizobiums pourraient être mis à contribution, y compris l'accélération de la croissance des plantes et l'aptitude à dégrader les polluants.

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Section: Rhizobium Leguminosarumsupporting

confidence: 73%

Diversity of symbiotic rhizobia resident in Canadian soils

Bromfield¹

2003

Can. J. Soil. Sci.

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“…A frequent problem is the failure of the inoculum strain to occupy a significant proportion of the nodules (Dudman and Brockwell, 1968;Bohlool and Schmidt, 1973;Dunigan et al, 1984;Moxley et al, 1986;Ramos and Boddey, 1987;Kucey, 1989;McLoughlin et al, 1990a,b;Wolff et al, 1991;Date, 1991;Slattery and Coventry, 1993;Carter et al, 1995). Inoculant rhizobia must often compete with the indigenous, often ineffective, rhizobial soil population.…”

Section: Documentation Of the Problemmentioning

confidence: 97%

Factors Influencing Nodule Occupancy by Inoculant Rhizobia

Vlassak

Vanderleyden

1997

Critical Reviews in Plant Sciences

115

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“…Vidor, personal communication). These often ineffective, indigenous organisms can limit inoculant-strain nodule occupancy and constrain nitrogen fixation (Graham 1981;Huntington et al 1986;Moxley et al 1986). The situation is analogous to that occurring with soybean in the American Midwest, where inoculant bradyrhizobia rarely give rise to more than 20% of the nodules formed and symbiotic nitrogen fixation may supply less than 40% of the plant's nitrogen needs Frederick 1974a, 1974b; Can.…”

Section: Introductionmentioning

confidence: 98%

Preference in the nodulation of Phaseolus vulgaris cultivar RAB39

Montealegre¹,

Graham²,

Kipe-Nolt³

1995

Can. J. Microbiol.

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The low nodule occupancy achieved by superior nitrogen-fixing inoculant strains is a problem in the production of many traditional legume species, including Phaseolus vulgaris. Cultivars that select for inoculant strains, rather than nodulate with ineffective indigenous rhizobia, offer one approach to the resolution of this problem. In this study we identify a bean cultivar, RAB39, that nodulates preferentially with Rhizobium tropici, including the inoculant-quality strain UMR1899. This preference in nodulation was not affected by temperature or pH, though strain preference by a second, alkaline-producing Rhizobium etli strain did vary markedly with temperature. When RAB39 was inoculated with 1:1 mixtures of UMR1899 and seven serologically distinct strains of Rhizobium etli, more than 75% of the nodules formed by each strain pair contained UMR1899. A number of studies have reported close correlations between the percent nodule occupancy and speed of nodulation, measured using the root-tip-marking procedure. Exceptions to this correlation were evident in the present study, suggesting that host preference in nodulation was not related to differences in the parameters normally used to estimate speed of nodulation. The preference of RAB39 for R. tropici, a species of Rhizobium that occurs at low frequency in most bean soils, and especially for the inoculant-quality strain UMR1899, provides a tool to overcome the lack of response to inoculation in common bean.Resume : La faible occupation des nodositCs rCalisCe par les souches inoculantes fixatrices d'azote constitue un problbme pour la production de plusieurs espbces de 1Cgumineuses traditionnelles, incluant Phaseolus vulgaris. Des cultivars qui ~Clectionnent des souches inoculantes plut6t que de produire des nodositks avec des rhizobia indigbnes inefficaces offrent une approche pour rCsoudre ce problkme. Dans cette Ctude, un cultivar de haricot, le RAB39, qui produit des nodositks de f a~o n prCfCrentielle avec le Rhizobium tropici, incluant la souche de qualit6 UMR1899, a Ct C identifiC. Cette prCfCrence pour la nodulation n'a pas Ct C affectCe par la tempCrature ou par le pH, bien que sa prCfCrence pour une autre souche, une souche productrice alcaline, le Rhizobium etli, ait vari6 de f a~o n marquCe avec la temperature. Lorsque le RAB39 a Ct C inoculC avec des mClanges 1: 1 de UMR1899 et de sept souches s6rologiquement distinctes de Rhizobium etli, plus de 75% des nodositks formCes par chaque paire de souches contenaient le UMR1899. Certaines Ctudes ont rapport6 des corrClations Ctroites entre le pourcentage de nodosit6s et la rapidit6 des nodulations, mesurCe par des marqueurs de pointes racinaires. Des exceptions B cette corrClation ont Ct C Cvidentes dans la prCsente Ctude, ce qui suggbre que la prCfCrence d'un h6te dans la nodulation n'est pas reliCe aux diffkrences de parambtres normalement utilisCs pour Cvaluer la rapidit6 des nodulations. La prCfCrence du RAB39 pour le R. tropici, une espbce de Rhizobium dont la frkquence est faible dans la plupart des sol...

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N₂ FIXATION AND COMPETITIVENESS OF Rhizobium phaseoli STRAINS ISOLATED FROM ONTARIO SOILS

Abstract: Moxr-By, J. C., Hurr

Cited by 21 publications

References 17 publications

Diversity of symbiotic rhizobia resident in Canadian soils

Diversity of symbiotic rhizobia resident in Canadian soils

Factors Influencing Nodule Occupancy by Inoculant Rhizobia

Preference in the nodulation of Phaseolus vulgaris cultivar RAB39

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N2 FIXATION AND COMPETITIVENESS OF Rhizobium phaseoli STRAINS ISOLATED FROM ONTARIO SOILS

Abstract: Moxr-By, J. C., Hurr

Cited by 21 publications

References 17 publications

Diversity of symbiotic rhizobia resident in Canadian soils

Diversity of symbiotic rhizobia resident in Canadian soils

Factors Influencing Nodule Occupancy by Inoculant Rhizobia

Preference in the nodulation of Phaseolus vulgaris cultivar RAB39

Contact Info

Product

Resources

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N₂ FIXATION AND COMPETITIVENESS OF Rhizobium phaseoli STRAINS ISOLATED FROM ONTARIO SOILS