N-Terminal Derivatization of Peptides with 4'-Formylbenzo-18-crown-6-ether for Protein and Species Identification
Luka Ozdanovac,
Renata Biba,
Marijana Erk
et al.
Abstract:Our research objective was to investigate the use of 4 '-formylbenzo-18-crown-6-ether (4fb18C6) for the covalent labelling of peptides for unambiguous peptide identification. Specifically, 23 peptides were analysed using a coupled system of liquid chromatography and tandem mass spectrometry with electrospray ionization to test de novo sequencing of derivatized and intact peptides. The reaction was optimized for reductive amination to be performed in an aqueous medium at pH 6 using the microwave radiation. Aft… Show more
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