N-terminal fragment of the anti-angiogenic human endostatin binds copper(II) with very high affinity

Tuning the coordination properties of multi-histidine peptides by using a tripodal scaffold: solution chemical study and catechol oxidase mimicking

“…Linear peptides have long since been used to mimic the metal binding sites of metalloproteins [1][2][3][4][5][6][7] and metalloenzymes. [8][9][10][11][12] Previously, we also studied some histidine-rich peptides in order to create similar metal ion environment to native enzymes.…”

“…[8][9][10][11][12] Previously, we also studied some histidine-rich peptides in order to create similar metal ion environment to native enzymes. 1,6,[8][9][10][11] Such metallopeptides may possess notable catalytic activity to promote either hydrolytic 8,14 or redox [9][10][11][12][13] reactions. However, from structural point of view, linear peptides may only be useful to mimic metal binding sites of native enzymes, if the residues involved in the coordination are part of a relatively short sequence.…”

A minimalist chemical model of matrix metalloproteinases — Can small peptides mimic the more rigid metal binding sites of proteins?

“…Such metal binding sites are obviously difficult to mimic by small peptides. However, a number of proteins possess relatively short histidine-rich sequences with strong metal binding ability, which substantially contributes to the function of the given macromolecule [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18]. Beside the well known human serum albumin (HSA) [1], probably the prion proteins (PrP) [2] are the most studied examples of such sequences.…”

“…Besides, several peptides copying the putative metal binding sequences of e.g. human α-synuclein [9], human endostatin [10], bacterial superoxide dismutases [11,12] and E. coli SlyD protein [13] have been studied to uncover fine functional details of the corresponding proteins. However, these metal binding sequences are part of less structured regions, frequently at the N-terminals, which may allow closer analogy in the metal binding properties of model peptides and native proteins.…”

Polidentát tripodális ligandumok biomimetikus fémkomplexei

“…[9][10][11][12][13][14] Az enzimek funkcionális modellezésére a peptid komplexek kézenfekvõ választásnak tûnnek, hiszen elvileg az aktív centrumhoz nagyon hasonló fémion környezet alakítható ki. Ennek ellenére a SOD enzim kivételével 10 kevés példa akad az irodalomban enzim mimetikus vizsgálatokra.…”