N-terminal sequences from
            <i>Autographa californica</i>
            nuclear polyhedrosis virus envelope proteins ODV-E66 and ODV-E25  are sufficient to direct reporter proteins to the nuclear  envelope, intranuclear microvesicles and the envelope  of occlusion derived virus

Hong, Tao; Summers, Max D.; Braunagel, Sharon C.

doi:10.1073/pnas.94.8.4050

Cited by 96 publications

(97 citation statements)

References 25 publications

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“…As seen in Fig. 4(g-i), local accumulation of GFP-ODV-E25 and P91-DsRed exhibited some degree of overlap, suggesting that these two proteins were, as reported previously, co-ordinately assembled in the ODV envelopment sites to generate ODVs (Hong et al, 1997;Russell & Rohrmann, 1997).…”

Section: Two Odv-associated Proteins P91-gfp and Gfp-odv-e25 Become supporting

confidence: 80%

“…4 j-o). Furthermore, previous studies have shown that the ODV-associated proteins accumulate at the periphery of the virogenic stroma (Russell & Rohrmann, 1993, 1997Hong et al, 1997). Taken together, these two independent observations lead to speculation that IE1 associates with the virogenic stroma.…”

Section: Two Odv-associated Proteins P91-gfp and Gfp-odv-e25 Become mentioning

confidence: 73%

“…ODV-E25, ODV-E66, P91 and P74) were shown to localize to the periphery of the nucleus (Russell & Rohrmann, 1997;Braunagel et al, 1999;RosasAcosta et al, 2001;Slack et al, 2001). More detailed observations with immunoelectron microscopy, however, have revealed that these proteins are mainly localized to the peristromal region in association with ODVs and intranuclear microvesicles (Russell & Rohrmann, 1993, 1997Braunagel et al, 1996a, b;Hong et al, 1997). The intranuclear microvesicles are the proposed source of ODV envelopes (Hong et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

“…More detailed observations with immunoelectron microscopy, however, have revealed that these proteins are mainly localized to the peristromal region in association with ODVs and intranuclear microvesicles (Russell & Rohrmann, 1993, 1997Braunagel et al, 1996a, b;Hong et al, 1997). The intranuclear microvesicles are the proposed source of ODV envelopes (Hong et al, 1997). Thus, the peripheral regions of the nucleus in which ODV-associated proteins often accumulate are considered to be the peristromal regions for intranuclear envelopment and are assumed to be a subnuclear compartment distinct from the virogenic stroma (Hong et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

“…The intranuclear microvesicles are the proposed source of ODV envelopes (Hong et al, 1997). Thus, the peripheral regions of the nucleus in which ODV-associated proteins often accumulate are considered to be the peristromal regions for intranuclear envelopment and are assumed to be a subnuclear compartment distinct from the virogenic stroma (Hong et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

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Analysis of baculovirus IE1 in living cells: dynamics and spatial relationships to viral structural proteins

Kawasaki

Matsumoto

Nagamine

2004

Journal of General Virology

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IE1, a principal transcriptional activator of the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV), is an essential factor for viral DNA replication. During viral infection, IE1 accumulates in discrete subnuclear structures where viral DNA replication occurs. To analyse the dynamic properties of IE1, we monitored green fluorescent protein-tagged IE1 (IE1-GFP) in BmNPV-infected B. mori cells by live-cell microscopy. Time-lapse imaging showed that IE1-associated structures gradually expanded and occasionally fused with one another, while photobleaching experiments revealed that IE1-GFP was relatively immobile inside the IE1-associated structures. To investigate the spatial relationships between IE1 and viral structural proteins in infected cells, three GFP-tagged viral components were expressed together with DsRed-tagged IE1. Two structural proteins that constitute the occlusion-derived virus (ODV), P91-GFP and GFP-ODV-E25, localized to the periphery of the IE1-associated structures. While local accumulations of these proteins were often in contact with the IE1-associated structures, they did not extend beyond the boundaries of the structures. In contrast, the major capsid protein VP39-GFP predominantly accumulated within the IE1-associated structures. These data indicated, in conjunction with the finding of a high DNA content in the structures, that IE1 localizes to the virogenic stroma and therefore support the prediction previously proposed that the virogenic stroma is a site for viral DNA replication as well as for the assembly of nucleocapsids.

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Section: Two Odv-associated Proteins P91-gfp and Gfp-odv-e25 Become supporting

confidence: 80%

Section: Two Odv-associated Proteins P91-gfp and Gfp-odv-e25 Become mentioning

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Analysis of baculovirus IE1 in living cells: dynamics and spatial relationships to viral structural proteins

Kawasaki

Matsumoto

Nagamine

2004

Journal of General Virology

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Autographa californicaNuclear Polyhedrosis Virus: Subcellular Localization and Protein Trafficking of BV/ODV-E26 to Intranuclear Membranes and Viral Envelopes

1998

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The Autographa californica nuclear polyhedrosis virus da26 gene codes for an envelope protein of both budded virus (BV) and occlusion derived virus (ODV). Western blot and temporal analysis of infected cell extracts detected a protein of 26 kDa by 4 h postinfection (p.i.). The amount of protein increased by 16 h p.i. and remained at high levels throughout infection. By 36 h p.i. several additional immunoreactive proteins were detected which migrated at approximately 18 kDa and remained through 96 h p.i. Western blot analysis of purified virus envelope and nucleocapsid preparations revealed that both the 26- and 18-kDa proteins are structural proteins of the envelope of BV and ODV. Immunoelectron microscopy performed at a time when only the 26-kDa species of the protein was present confirmed that the protein located to ODV envelope. The protein was named BV/ODV-E26 to designate incorporation into viral progeny, envelope location, and apparent molecular weight. Studies designed to follow localization of BV/ODV-E26 demonstrated that early in infection, the protein was incorporated into cytoplasmic vesicles and by 16 h p.i., BV/ODV-E26 was detected in the nucleus associated with virus-induced intranuclear microvesicles and ODV envelope. Coimmunoprecipitation and yeast two-hybrid assays showed that BV/ODV-E26 and FP25K were capable of interacting with each other to form a complex and coimmunoprecipitation assays indicated that cellular actin was a third component of this complex. Together, these data suggest that FP25K and cellular actin may participate in the regulation, or movement through the cell, of baculovirus proteins and/or virus nucleocapsids.

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Autographa californicaNucleopolyhedrovirus Infection Results in Sf9 Cell Cycle Arrest at G2/M Phase

et al. 1998

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Baculovirus infection results in the induction of membrane structures within the nucleoplasm of the host cells. The source of these membranes is unclear; however, using the normal dynamics of cellular membranes and the nuclear envelope as a model, it is possible that the cell cycle might play a role in the regulation of formation of these intranuclear membranes. Therefore, one goal of this study was to investigate the effect of baculovirus infection on the cell cycle of Sf9 host cells. Since few data are available on the cell cycle of insect cells, the first task was to define Sf9 cell cycle kinetics. The cell cycle phase distribution of Sf9 cells grown in suspension culture was determined to be evenly distributed (29% of the cells in G1, 33% in S, and 36% in G2/M phase), with the duration of G1 and S phases both being about 6 h and the combined duration of G2/M phase being about 8 h. When Sf9 cells were infected with AcMNPV (Autographa californica nuclear polyhedrosis virus), approximately 84% of the cells were arrested in G2/M phase by 18-24 h p.i. Concomitant with the viral-induced arrest in G2/M phase, high levels of both cdc2-associated histone H1 kinase activity and cyclin B protein were detected. By 24 h p.i. cyclin B was no longer detected; however, cdc2-associated histone H1 kinase activity remained throughout the infection. These data suggested that early in infection, cyclin B/cdc2 complex may be used to regulate the transition from G2 to M phase, but prolonged arrest may be due to a protein(s) encoded by AcMNPV. DNA hybridization analysis showed that the maximal rate of viral DNA replication occurred before G2/M arrest. We noted that viral DNA replication still occurred late in infection, when the majority of the cells were arrested in G2/M phase. Since cellular DNA replication normally does not occur during G2 or M phase, experiments were designed to determine if viral DNA replication could occur even when host cell DNA replication was arrested. Sf9 cells were arrested and "frozen" at the boundary of G1/S phase using 5-fluoro-2'deoxyuridine (FdUrd) treatment and then infected with AcMNPV In the blocked, infected cells, viral DNA replication was detected; however, cellular DNA remained at steady-state levels. These results suggested that cellular DNA replication was not necessary for viral DNA replication and show that viral DNA replication was not significantly inhibited by FdUrd treatment. It was a surprise to detect viral DNA replication when the host cells were "frozen" at G1/S phase. We wanted to determine if the viral infection was progressing to the stage of progeny virus production. Our data showed that progeny budded virus (BV) and virus-induced intranuclear microvesicles were produced in the frozen, infected cells; however, the intranuclear microvesicles had an unusual structure. They were irregular in shape and thickened compared to those observed in a normal infection. Very few enveloped nucleocapsids were visible in the nucleus of the frozen, infected cells and the occluded-derived virus ...

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N-terminal sequences from Autographa californica nuclear polyhedrosis virus envelope proteins ODV-E66 and ODV-E25 are sufficient to direct reporter proteins to the nuclear envelope, intranuclear microvesicles and the envelope of occlusion derived virus

Cited by 96 publications

References 25 publications

Analysis of baculovirus IE1 in living cells: dynamics and spatial relationships to viral structural proteins

Analysis of baculovirus IE1 in living cells: dynamics and spatial relationships to viral structural proteins

Autographa californicaNuclear Polyhedrosis Virus: Subcellular Localization and Protein Trafficking of BV/ODV-E26 to Intranuclear Membranes and Viral Envelopes

Autographa californicaNucleopolyhedrovirus Infection Results in Sf9 Cell Cycle Arrest at G2/M Phase

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