N46 Animal models of overactive bladder: Cyclophosphamide (CYP)-induced cystitis in rats

Juszczak, Kajetan; Wyczółkowski, Marek; Thor, Piotr

doi:10.1016/s1569-9056(09)74821-3

Cited by 14 publications

(32 citation statements)

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“…O/mL) (in group I and IV) and DOI -detrusor overactivity index (cm H 2 O/mL) (in group II and III), depicted as a quotient of the sum of amplitudes of all detrusor contractions during the filling phase and functional bladder capacity. 11 The intravesical administration of DMSO solution has no significant effect on motor and sensory urinary bladder activity (group V), thus this group was excluded from further analysis.…”

Section: Experimental Protocolmentioning

confidence: 99%

The angiotensin II receptors type 1 blockage affects the urinary bladder activity in hyperosmolar-induced detrusor overactivity in rats: Preliminary results

Juszczak¹,

Maciukiewicz²

2017

Adv Clin Exp Med

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Section: Experimental Protocolmentioning

confidence: 99%

The angiotensin II receptors type 1 blockage affects the urinary bladder activity in hyperosmolar-induced detrusor overactivity in rats: Preliminary results

Juszczak¹,

Maciukiewicz²

2017

Adv Clin Exp Med

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“…Other scientists have described in vivo experiments on detrusor overactivity in animal models using cyclophosphamide and hyperosmolar stimuli [11,12]. However, still there are no data regarding the influence of cyclophosphamide and hyperosmolar stimuli on the culture of normal rat urothelial cells in vitro.…”

Section: Discussionmentioning

confidence: 99%

“…It is believed that cyclophosphamide and hyperosmolar urine may activate afferent fibres directly and/or indirectly via urothelium, leading to DO. Additionally, previous in vivo studies showed that hyperosmolar intravesical stimulation and cyclophosphamide treatment (in acute and chronic models of cystitis) induce detrusor overactivity [11,12]. Kulick et al reported that the mean, the lowest and the highest values of rat's urine osmolarity were 2080, 1553 and 3222 mOsm/l, respectively [13].…”

Section: Introductionmentioning

confidence: 99%

“…Kulick et al reported that the mean, the lowest and the highest values of rat's urine osmolarity were 2080, 1553 and 3222 mOsm/l, respectively [13]. In the acute model of cyclophosphamideinduced cystitis, DO developed 4 hours after cyclophosphamide administration [11]. Unfortunately, the influence of cyclophosphamide and hyperosmolar stimuli on urothelium is not well described.…”

Section: Introductionmentioning

confidence: 99%

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The effect of hyperosmolar stimuli and cyclophosphamide on the culture of normal rat urothelial cells in vitro

Juszczak¹,

Kaszuba-Zwoińska

Chorobik

et al. 2012

Cellular and Molecular Biology Letters

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Highly concentrated urine may induce a harmful effect on the urinary bladder. Therefore, we considered osmolarity of the urine as a basic pathomechanism of mucosal damage. The influence of both cyclophosphamide (CYP) and hyperosmolar stimuli (HS) on the urothelium are not well described. The purpose was to evaluate the effect of CYP and HS on rat urothelial cultured cells (RUCC). 15 Wistar rats were used for RUCC preparation. RUCC were exposed to HS (2080 and 3222 mOsm/l NaCl) for 15 min and CYP (1 mg/ml) for 4 hrs. APC-labelled annexin V was used to quantitatively determine the percentage of apoptotic cells and propidium iodide (PI) as a standard flow cytometric viability probe to distinguish necrotic cells from viable ones. 23.7 ± 3.9% and 26.0 ± 1.5% apoptotic cells, respectively, 14.3 ± 1.4% and 19.4 ± 2.7% necrotic cells, respectively and 60.5 ± 1.4% and 48.6 ± 5.3% dead cells, respectively. The effect of CYP on RUCC was similar to the effect of HS. After CYP the apoptotic and necrotic cells were 23.1 ± 0.3% and 17.9 ± 7.4%, respectively. The percentage of dead cells was 57.7 ± 10.8%. CYP and HS induced apoptosis and necrosis in RUCC. 3222 mOsm/l HS had the most harmful effect based on the percentage of necrotic and apoptotic cells.

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“…The free end of the implanted catheter was connected via T-stopcock to a pressure transducer (UFI, MorroBay, CA, USA) and injection pump (Unipan 340A, Poland). Cystometry was recorded using ML110-BridgeAmp (ADInstruments, Australia) hardware and PowerLab/8SP (ADInstruments, Castle Hill, Australia) software, as previously described (14,17). Study protocol.…”

Section: Animalsmentioning

confidence: 99%

The Ameliorating Effect of Exogenous Melatonin on Urinary Bladder Function in Hyperosmolar Bladder Overactivity and its Influence on the Autonomic Nervous System Activity

Juszczak

Ziomber

Machowska

et al. 2011

Acta Med. (Hradec Kralove, Czech Repub.)

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The pathophysiology of overactive bladder (OAB) is multifactorial. Although, the two pivotal myogenic (alterations in bladder smooth muscle contractility and excitability) and neurogenic (increased or sensitized sensory transmission, abnormalities in spinal and supraspinal control) basis for the OAB development have been proposed (4,8,28). The neurogenic theory explains that the backgrounds for OAB seems to be partially related to alterations in bladder afferent nerve fibres localized within the bladder mucosa.Animal studies revealed that the bladder mucosa is characterized by a significantly lower concentration of highenergy phosphates and higher oxidative metabolic rate than the detrusor smooth muscle. This facts point that the bladder mucosa is significantly more sensitive to anoxia than is the bladder smooth muscle (13,18). Azadzoi et al. (2) study revealed that long-term arterial insufficiency leads to bladder neuropathy via ischemia, hypoxia and oxidative stress. OAB under the ischemic condition generates neurotoxic oxidative and nitrosative products. Neurodegeneration is postulated to be an end stage phenomenon in ischemia induced bladder overactivity. The increased sensitivity of urothelium to different stimuli (e.g. hypoxia, ischemia, hyperosmotic agents, etc.) would indicate that bladder afferents (pain, altered sensations, reflex contractions) might be activated at levels of hypoxia/ischemia that have no direct effect on bladder smooth muscle function.Therefore, a novel strategies for therapeutic approach of OAB based on: 1) reducing the phasic increase in intracellular calcium that causes the activation of calcium activated hydrolytic enzymes, 2) stabilizing the neuronal or smooth muscle membrane components that are hydrolyzed by the activated enzymes; or 3) employing free radical scavengers that reduce the activation of cellular lipid peroxidases and the reperfusion injury, seem to be crucial (19 Summary: This study was designed to investigate the effects of melatonin on the bladder hyperactivity in hyperosmolar-induced overactive bladder (OAB) rats. Additionally, the influence of melatonin on the autonomic nervous system (ANS) using heart rate variability (HRV) analysis was assessed. 40 rats were divided into four groups: I -control (n=12), II -rats with hyperosmolar OAB (n=6), III -rats with melatonin pretreatment and hyperosmolar OAB (n=6) and IV -control with melatonin pretreatment (n=6). In group III and IV melatonin in dose of 100 mg/kg was given. HRV measurements in 10 rats, as follow: control (n=2), control after melatonin treatment (n=2), rats with hyperosmolar OAB without (n=3), and after (n=3) melatonin treatment were conducted. This study demonstrates marked influence of melatonin on urinary bladder activity in hyperosmolar-induced OAB rats. These rats showed significantly reduced the detrusor motor overactivity resulting in the improvement of cystometric parameters after melatonin treatment when compared to the control, as follow: a significant increase of intercontraction interval (70 ...

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N46 Animal models of overactive bladder: Cyclophosphamide (CYP)-induced cystitis in rats

Cited by 14 publications

References 0 publications

The angiotensin II receptors type 1 blockage affects the urinary bladder activity in hyperosmolar-induced detrusor overactivity in rats: Preliminary results

The angiotensin II receptors type 1 blockage affects the urinary bladder activity in hyperosmolar-induced detrusor overactivity in rats: Preliminary results

The effect of hyperosmolar stimuli and cyclophosphamide on the culture of normal rat urothelial cells in vitro

The Ameliorating Effect of Exogenous Melatonin on Urinary Bladder Function in Hyperosmolar Bladder Overactivity and its Influence on the Autonomic Nervous System Activity

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