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Abstract

Treatment of bacterial infections with chemicals has led to drug resistance. Therefore, research to replace herbal treatments with less side effects is of a great importance. Therefore, this study aims at investigating the antimicrobial effects of methanolic extract of Rosmarinus officinalis on some gram positive and gram negative bacteria. In this research study, after collecting the plant and confirming its scientific name, R. officinalis extract was prepared using Soxhlet extractor method at the concentrations of 20-400 mg/mL and the antimicrobial effects of the extract using agar well diffusion and determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) from dilution method against standard bacteria of Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa. Methanolic extract of R . officinalis plant has an inhibitory effect on P. aeruginosa, E. coli and S. aureus so that it has the highest sensitivity to methanolic extracts of R. officinalis in P. aeruginosa with a 19.8 mm the zone of growth inhibition and the lowest sensitivity to S. aureus with the zone of growth inhibition 14.4 mm. The results of this study showed that R. officinalis extract has a significant effect on tested bacteria, and further research is required to identify, quantify, and purify its effective compounds.

Eco-Friendly Solution Based on Rosmarinus officinalis Hydro-Alcoholic Extract to Prevent Biodeterioration of Cultural Heritage Objects and Buildings

“…The chosen extraction method was based on the study conducted by Švarc-Gajić et al, in 2013 [ 40 ], where the polyphenol content differs depending on the solvent used. Higher phenol yields were obtained by using methanol, which is the most widely used solvent in the literature [ 41 , 42 ] but with a strong toxic effect, so it is not recommended to use [ 43 ]. The lowest extraction yield was obtained for ethyl acetate, even if the dipole moment is not very different from methanol (1.78 and 1.69, respectively) [ 40 ].…”

Green Synthesis of Gold Nanoflowers Using Rosmarinus officinalis and Helichrysum italicum Extracts: Comparative Studies of Their Antimicrobial and Antibiofilm Activities

“…Numerous studies have documented the antimicrobial activity of H. italicum [ 30 , 40 , 46 ] and R. officinalis extracts [ 47 , 48 , 49 ]. To our knowledge, a few recent studies have also focused on nanoparticle synthesis using rosemary extracts as reductants [ 20 , 50 , 51 ], yet there is no literature knowledge about nanoparticle synthesis from immortelle extracts.…”