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Abstract

Objective:To explore the effects of glucose concentration on growth of Wharton's jelly mesenchymal stem cells from umbilical cords of gestational diabetic mothers (DWJMSCs) and normal mothers (NWJMSCs). Methods: NWJMSCs and DWJMSCs were treated at passage 3 with low (5.5mM) or high glucose. Cells' growth was evaluated by methyl thiazolyl tetrazolium uptake and cytotoxicity by LDH release. Status of oxidative stress was estimated by release of stress enzymes i.e. glutathione s transferase (GSH) and catalase. Results:The results of cells' growth assays indicated a significant increase of proliferation in DWJMSCs when treated with high glucose as compared to low glucose while opposite was found in NWJMSCs. Conversely cytotoxicity was lower in DWJMSCs treated with 25mM glucose while it was increased in NWJMSCs at same concentration and vice-versa. Both groups of cells exhibited similar behavior in release of antioxidant enzymes i.e. GSH reduced in both cell types at 25mM glucose while no difference was observed in catalase activity. Conclusion: Growth of DWJMSCs is enhanced at high glucose concentrations while it is reduced in NWJMSCs.