Na+/K+-ATPase, acetylcholinesterase and glutathione S-transferase activities as new markers of postmortem interval in Swiss mice

Fonseca, Caren A.R. da; Paltian, Jaini J.; Reis, Angélica S.; Bortolatto, Cristiani F.; Wilhelm, Ethel A.

doi:10.1016/j.legalmed.2018.11.003

Cited by 12 publications

(4 citation statements)

References 55 publications

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“…Significant progress has been achieved in the analysis of postmortem protein decomposition patterns for PMI estimation in recent years (e.g. [43][44][45][46]). This interest is especially the case for muscle protein degradation in animal surrogates [47][48][49][50] and humans [11,48].…”

Section: Proteinsmentioning

confidence: 99%

The applicability of forensic time since death estimation methods for buried bodies in advanced decomposition stages

et al. 2020

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Estimation of the postmortem interval in advanced postmortem stages is a challenging task. Although there are several approaches available for addressing postmortem changes of a (human) body or its environment (ecologically and/or biochemically), most are restricted to specific timeframes and/or individual and environmental conditions. It is well known, for instance, that buried bodies decompose in a remarkably different manner than on the ground surface. However, data on how established methods for PMI estimation perform under these conditions are scarce. It is important to understand whether and how postmortem changes are affected under burial conditions, if corrective factors could be conceived, or if methods have to be excluded for respective cases. We present the first multi-methodological assessment of human postmortem decomposition carried out on buried body donors in Europe, at the Amsterdam Research Initiative for Sub-surface Taphonomy and Anthropology (ARISTA) in the Netherlands. We used a multidisciplinary approach to investigate postmortem changes of morphology, skeletal muscle protein decomposition, presence of insects and other necrophilous animals as well as microbial communities (i.e., microbiomes) from August to November 2018 associated with two complete body exhumations and eight partial exhumations. Our results clearly display the current possibilities and limitations of methods for PMI estimation in buried remains and provide a baseline for future research and application.

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Section: Proteinsmentioning

confidence: 99%

The applicability of forensic time since death estimation methods for buried bodies in advanced decomposition stages

et al. 2020

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“…This constitutes a major advantage in both research (e.g., multiple samples can be taken and analyzed, without interference by previous samplings) and practical application (e.g., even in severely injured bodies or body parts, there is a high chance to be able to collect unaffected tissue for analysis). Available data, however, is largely limited to thigh muscle [ 5 – 8 ], with few exceptions (e.g., M. psoas [ 9 ], M. gastrocnemius [ 10 ]). To fully benefit from the described advantages of muscle tissue, further research is required regarding decomposition similarities and deviations within individual muscles, between muscles, and between muscle types (skeletal muscle, cardiac and smooth muscle).…”

Section: Introductionmentioning

confidence: 99%

Intra- and intermuscular variations of postmortem protein degradation for PMI estimation

et al. 2020

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In recent years, protein decomposition has become of increasing interest for the use in forensic estimation of the postmortem interval (PMI). Especially skeletal muscle tissue has proven to be a prime target tissue, among other reasons, due to its large abundance in the human body. In this regard, it is important to know whether there are any intra-and intermuscular differences in the behavior of protein degradation. Thus, samples from different locations within several skeletal muscles as well as from cardiac and smooth muscle tissue samples were collected from three autopsy cases with varying degree of decomposition. Samples were analyzed by SDS-PAGE and Western blotting and compared for protein degradation patterns. Intramuscular variations turned out to be minimal and without major influence for the use of the method. Observed intermuscular differences provide possibilities for future improvement of the precision and temporal application range. The results of this study show the strengths and current limitations of protein degradation-based PMI estimation and provide a deeper understanding of intraindividual postmortem protein degradation processes.

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“…Most literature concerning AChE activity is controversial. It contained a variety of AChE activity that increased, decreased and even unchanged at different postmortem sampling times [27] , [28] , [29] . It was observed that oxidative damage in the heart started earlier than in the kidneys; different antioxidant activities attributed to the different damage onset in both organs [30] .…”

Section: Discussionmentioning

confidence: 99%

Postmortem sampling time effect on toxicity biomarkers in rats exposed to an acute lethal methomyl dose

et al. 2022

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Na+/K+-ATPase, acetylcholinesterase and glutathione S-transferase activities as new markers of postmortem interval in Swiss mice

Cited by 12 publications

References 55 publications

The applicability of forensic time since death estimation methods for buried bodies in advanced decomposition stages

The applicability of forensic time since death estimation methods for buried bodies in advanced decomposition stages

Intra- and intermuscular variations of postmortem protein degradation for PMI estimation

Postmortem sampling time effect on toxicity biomarkers in rats exposed to an acute lethal methomyl dose

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