Na-Montmorillonite-Dispersed Sustainable Polymer Nanocomposite Hydrogel Films for Anticancer Drug Delivery

Abstract

Nanocomposite hydrogels have found a wide scope in regenerative medicine, tissue engineering, and smart drug delivery applications. The present study reports the formulations of biocompatible nanocomposite hydrogel films using carboxymethyl cellulose-hydroxyethyl cellulose-acrylonitrile-linseed oil polyol (CHAP) plain hydrogel and Na-montmorillonite (NaMMT) dispersed CHAP nanocomposite hydrogel films (NaCHAP) using solution blending technique. The structural, morphological, and mechanical properties of resultant nanocomposite hydrogel films were further investigated to analyze the effects of polyol and NaMMT on the characteristic properties. The synergistic effect of polyol and nanofillers on the mechanical strength and sustained drug-release behavior of the resultant hydrogel films was studied, which revealed that the increased cross-link density of hydrogels enhanced the elastic modulus (up to 99%) and improved the drug retention time (up to 72 h at both pHs 7.4 and 4.0). The release rate of cisplatin in nanocomposite hydrogel films was found to be higher in CHAP-1 (83 and 69%) and CHAP-3 (79 and 64%) than NaCHAP-3 (77 and 57%) and NaCHAP-4 (73 and 54%) at both pHs 4.0 and 7.4, respectively. These data confirmed that the release rate of cisplatin in nanocomposite hydrogel films was pH-responsive and increased with decrease of pH. All nanocomposite hydrogel films have exhibited excellent pH sensitivity under buffer solution of various pHs (1.0, 4.0, 7.4, and 9.0). The in vitro biocompatibility and cytotoxicity tests of these films were also conducted using 3-(4,5-dimethylthiazole-2-yl-2,5-diphenyl tetrazolium bromide) assay of human embryonic kidney (HEK-293) and human breast cancer (MCF-7) cell lines up to 48 h, which shows their biocompatible nature. However, cisplatin-loaded nanocomposite hydrogel films effectively inhibited the growth of human breast MCF-7 cancer cells. These studies suggested that the proposed nanocomposite hydrogel films have shown promising application in therapeutics, especially for anticancer-targeted drug delivery.

Antibiotic Delivery Strategies to Treat Skin Infections When Innate Antimicrobial Defense Fails

“…CMC can be blended and polymerized with other materials, including polyethylene glycol (PEG), to form hydrogel matrices [151]. CMC also exists in salt form (NaCMC) to form intermolecular crosslinks in a blended hydrogel matrix, whereas native CMC tends to form intramolecular links [182].…”

A review on polymer nanocomposite hydrogel preparation, characterization, and applications

“…An agarose/alginate hydrogel with polypyrrole NPs was developed for use as an infrared-sensitive drug release system [132]. Na-MMT dispersed in carboxymethyl cellulosehydroxyethyl cellulose-acrylonitrile-linseed oil polyol (CHAP) hydrogel matrices have proven to be promising for pHdependent anticancer drug delivery [133]. For the adsorption of textile dyes such as methylene blue [107], crystal violet [86], and heavy metals [105] from industrial waste, nanocomposite hydrogels have proven to be more efficient than conventional hydrogels.…”

Periodate-Modified Gum Arabic Cross-linked PVA Hydrogels: A Promising Approach toward Photoprotection and Sustained Delivery of Folic Acid

“…The FA release studies were carried out in triplicates and their average values were considered using following equation. 22 …”

“…The ratio of degradability was determined by calculating the differences of mass loss of these hydrogels by using the following equation. 22 = × W W % mass loss / 100…”