Na+/Ca2+ exchange regulates Ca2+-dependent duodenal mucosal ion transport and HCO3− secretion in mice

Dong, Hui; Sellers, Zachary M.; Smith, Anders; Chow, Jennifer; Barrett, Kim E.

doi:10.1152/ajpgi.00381.2004

Cited by 35 publications

(42 citation statements)

References 36 publications

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“…Although NCX expression and function have been demonstrated in intestinal epithelial cells (12) and in pancreatic ␤-islet cells (12), little is known about NCX in human pancreatic adenocarcinoma cells (22). Here, we demonstrated that both the mRNA transcript and protein of NCX1 are expressed in pancreatic cancer cells (Fig.…”

Section: Expression Of Ncx and Trpc Channel In Pancreatic Cancer Cellsmentioning

confidence: 60%

Molecular mechanisms underlying Ca²⁺-mediated motility of human pancreatic duct cells

Dong

Shim²,

et al. 2010

American Journal of Physiology-Cell Physiology

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Section: Expression Of Ncx and Trpc Channel In Pancreatic Cancer Cellsmentioning

confidence: 60%

Molecular mechanisms underlying Ca²⁺-mediated motility of human pancreatic duct cells

Dong

Shim²,

et al. 2010

American Journal of Physiology-Cell Physiology

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“…NCX1 is ubiquitously expressed in mammalian heart and brain tissue, where it is responsible for calcium homeostasis [43], although it is also widely expressed in other tissues [4,40]. NCX1 participates in essential processes such as balancing calcium ion concentrations by regulating duodenal mucosal ion transport and HCO3-secretion and controlling calcium ion homeostasis [16]. NCX1 also participates in cardiac muscle relaxation and in the maintenance of calcium concentration in the sarcoplasmic reticulum in cardiac cells [13].…”

Section: Discussionmentioning

confidence: 99%

Tissue-Specific Expression of the Calcium Transporter Genes TRPV5, TRPV6, NCX1, and PMCA1b in the Duodenum, Kidney and Heart of Equus caballus

Hwang

Jung

Yang

et al. 2011

The Journal of Veterinary Medical Science

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ABSTRACT. Calcium transporter genes, such as transient receptor potential cation channel subfamily V members 5/6 (TRPV5/6), Na + /Ca 2+ exchanger 1 (NCX1), and plasma membrane calcium-transporting ATPase 1b (PMCA1b), are essential for maintaining homeostasis and metabolizing Ca 2+ ions. The TRPV5 and TRPV6 proteins play an important role in Ca 2+ absorption, and NCX1 and PMCA1b are both critical for intracellular calcium homeostasis. In this study, the tissue-specific mRNA and protein expression of these calcium transporter genes in the duodenum, kidney and heart of the horse (Equus caballus) was examined using reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis. The tissue localization of these calcium transporters was also investigated using immunohistochemistry. The results showed that TRPV5 mRNA was highly expressed in the kidney but was scarce in the duodenum and heart. TRPV6 mRNA levels were similar in all the tissues. NCX1 and PMCA1b were both highly expressed in the heart, but no difference in NCX1 and PMCA1b mRNA expressions was observed in the duodenum and kidney. The aspect of protein expression was similar with mRNA expression data. Localization of calcium transporter genes were detected enterocytes in duodenum, the distal convoluted tubules in the kidney, and within the cardiac muscle cells of the heart. Based on these results, calcium transport genes appear to be expressed in horse tissues at levels similar to those observed in other vertebrates.KEY WORDS: calcium transporter gene, equine, NCX1, PMCA1b, TRPV.J. Vet. Med. Sci. 73(11): 1437-1444, 2011 Calcium is important for the overall maintenance of living organisms and is critical for strengthening bones, neurotransmitter release, and muscle contractions. It also functions as a second messenger, activates intracellular signaling, contraction and blood clotting and so on. Calcium deficiency results in symptoms such as muscle pain, muscle spasms, indigestion, diarrhea, an irregular menstrual cycle, pale skin, and listlessness. Deficiency can also result in osteoporosis, osteomalacia, rickets, and anemia [36]. Problems can also stem from absorbing too much calcium, including the formation of kidney stones, constipation and loss of appetite, nausea, seizures and even coma. Excess calcium retention can also lead to decreased absorption of other minerals [1]. Therefore, maintaining an appropriate calcium concentration is essential.The regulation of calcium concentration is controlled by three factors: parathyroid hormone (PTH), vitamin D, and calcitonin. The main role of PTH is to elevate blood calcium levels when required. PTH release causes the gastrointestinal tract to absorb more calcium, and it also triggers the release of calcium stored in bones and stimulates the kidney to excrete more phosphorous [41]. Vitamin D works in concert with PTH in bone and the kidneys and is necessary for intestinal calcium absorption [24]. The role of calcitonin is opposite to that of PTH, i.e., it decreases the calcium concentration in...

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“…Epithelial HCO 3 Ϫ and Cl Ϫ secretion is mainly under the control of cyclic AMP and Ca 2ϩ signaling, which may interact and cross-talk to regulate epithelial ion transports (25,42,53,54). Previous studies demonstrated that most well known secretagogues, such as forskolin, ACh, 5-HT, and PGE 2 , usually stimulate intestinal HCO 3 Ϫ and Cl Ϫ secretion in parallel (20,42,55,56). It is not known, however, whether epithelial HCO 3 Ϫ and Cl Ϫ secretion has to occur in parallel and whether they are regulated by the same or different signaling/mechanisms.…”

Section: Diated Intestinal Transepithelial Hcomentioning

confidence: 99%

Molecular Mechanisms of Calcium-sensing Receptor-mediated Calcium Signaling in the Modulation of Epithelial Ion Transport and Bicarbonate Secretion

Xie

Dong

Wong

et al. 2014

Journal of Biological Chemistry

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Background: Calcium-sensing receptor (CaSR) plays a critical role in the regulation of epithelial ion transport. Results: CaSR activators induce Ca 2ϩ signaling and duodenal bicarbonate secretion (DBS). Conclusion: CaSR triggers Ca 2ϩ -dependent DBS, likely through receptor-operated channels, intermediate conductance Ca 2ϩ -activated K ϩ channels, and the cystic fibrosis transmembrane conductance regulator.

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Na⁺/Ca²⁺ exchange regulates Ca²⁺-dependent duodenal mucosal ion transport and HCO₃⁻ secretion in mice

Abstract: Ca 2ϩ exchange regulates Ca 2ϩ -dependent duodenal mucosal ion transport and HCO 3 Ϫ secretion in mice.

Cited by 35 publications

References 36 publications

Molecular mechanisms underlying Ca²⁺-mediated motility of human pancreatic duct cells

Molecular mechanisms underlying Ca²⁺-mediated motility of human pancreatic duct cells

Tissue-Specific Expression of the Calcium Transporter Genes TRPV5, TRPV6, NCX1, and PMCA1b in the Duodenum, Kidney and Heart of Equus caballus

Molecular Mechanisms of Calcium-sensing Receptor-mediated Calcium Signaling in the Modulation of Epithelial Ion Transport and Bicarbonate Secretion

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Na+/Ca2+ exchange regulates Ca2+-dependent duodenal mucosal ion transport and HCO3− secretion in mice

Abstract: Ca 2ϩ exchange regulates Ca 2ϩ -dependent duodenal mucosal ion transport and HCO 3 Ϫ secretion in mice.

Cited by 35 publications

References 36 publications

Molecular mechanisms underlying Ca2+-mediated motility of human pancreatic duct cells

Molecular mechanisms underlying Ca2+-mediated motility of human pancreatic duct cells

Tissue-Specific Expression of the Calcium Transporter Genes TRPV5, TRPV6, NCX1, and PMCA1b in the Duodenum, Kidney and Heart of Equus caballus

Molecular Mechanisms of Calcium-sensing Receptor-mediated Calcium Signaling in the Modulation of Epithelial Ion Transport and Bicarbonate Secretion

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Na⁺/Ca²⁺ exchange regulates Ca²⁺-dependent duodenal mucosal ion transport and HCO₃⁻ secretion in mice

Molecular mechanisms underlying Ca²⁺-mediated motility of human pancreatic duct cells

Molecular mechanisms underlying Ca²⁺-mediated motility of human pancreatic duct cells