Na+/Ca2+ exchanger‐heterozygote knockout mice display increased relaxation in gastric fundus and accelerated gastric transit in vivo

Azuma, Yasutaka; Hayashi, Satomi; Nishiyama, Kazuhiro; Kita, Satomi; Mukai, Kusuhiro; Nakajima, Hidemitsu; Iwamoto, Takahiro; Takeuchi, Tadayoshi

doi:10.1111/nmo.12779

Cited by 14 publications

(16 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our second strength was to evaluate and analyze the motility according to the stimulus. We are confident in the used of these methods as our laboratory has already published many papers involving the use of an isotonic transducer under EFS (Azuma et al, ; Azuma, Samezawa, Nishiyama, Nakajima, & Takeuchi, ; Fujimoto et al, ).…”

Section: Discussionmentioning

confidence: 99%

Chronic kidney disease after 5/6 nephrectomy disturbs the intestinal microbiota and alters intestinal motility

Nishiyama

Aono

Fujimoto

et al. 2018

Journal Cellular Physiology

View full text Add to dashboard Cite

Organ-organ crosstalk is involved in homeostasis. Gastrointestinal symptoms are common in patients with renal failure. The aim of this study was to elucidate the relationship between gastrointestinal motility and gastrointestinal symptoms in chronic kidney disease. We performed studies in C57BL/6 mice with chronic kidney disease after 5/6 nephrectomy. Gastrointestinal motility was evaluated by assessing the ex vivo responses of ileum and distal colon strips to electrical field stimulation.Feces were collected from mice, and the composition of the gut microbiota was analyzed using 16S ribosomal RNA sequencing. Mice with chronic kidney disease after 5/6 nephrectomy showed a decreased amount of stool, and this constipation was correlated with a suppressed contraction response in ileum motility and decreased relaxation response in distal colon motility. Spermine, one of the uremic toxins, inhibited the contraction response in ileum motility, but four types of uremic toxins showed no effect on the relaxation response in distal colon motility. The 5/6 nephrectomy procedure disturbed the balance of the gut microbiota in the mice. The motility dysregulation and constipation were resolved by antibiotic treatments. The expression levels of interleukin 6, tumor necrosis factor-α, and iNOS in 5/6 nephrectomy mice were increased in the distal colon but not in the ileum. In addition, macrophage infiltration in 5/6 nephrectomy mice was increased in the distal colon but not in the ileum. We found that 5/6 nephrectomy altered gastrointestinal motility and caused constipation by changing the gut microbiota and causing colonic inflammation. These findings indicate that renal failure was remarkably associated with gastrointestinal dysregulation.

show abstract

Section: Discussionmentioning

confidence: 99%

Chronic kidney disease after 5/6 nephrectomy disturbs the intestinal microbiota and alters intestinal motility

Nishiyama

Aono

Fujimoto

et al. 2018

Journal Cellular Physiology

View full text Add to dashboard Cite

show abstract

“…In rat longitudinal gastric fundus muscle strips, a relaxant effect was produced by VIP, PACAP1–27, and secretin ( 79 ). Similarly, PACAP exerts relaxant activity on circular muscle strips from rat gastric fundus ( 80 ) and in the mouse antrum ( 81 ). In guinea pig gastric fundus, VIP, PACAP1-27 and PACAP1-38 induced concentration-dependent relaxation that was partly inhibited by the antagonists VIP10–28 and PACAP6–38 and the NO synthase inhibitor NG-nitro- l -arginine ( l -NNA).…”

Section: Effects Of Pacap In the Stomachmentioning

confidence: 99%

Presence and Effects of Pituitary Adenylate Cyclase Activating Polypeptide Under Physiological and Pathological Conditions in the Stomach

et al. 2018

View full text Add to dashboard Cite

Pituitary adenylate cyclase activating polypeptide (PACAP) is a multifunctional neuropeptide with widespread occurrence throughout the body including the gastrointestinal system. In the small and large intestine, effects of PACAP on cell proliferation, secretion, motility, gut immunology and blood flow, as well as its importance in bowel inflammatory reactions and cancer development have been shown and reviewed earlier. However, no current review is available on the actions of PACAP in the stomach in spite of numerous data published on the gastric presence and actions of the peptide. Therefore, the aim of the present review is to summarize currently available data on the distribution and effects of PACAP in the stomach. We review data on the localization of PACAP and its receptors in the stomach wall of various mammalian and non-mammalian species, we then give an overview on PACAP’s effects on secretion of gastric acid and various hormones. Effects on cell proliferation, differentiation, blood flow and gastric motility are also reviewed. Finally, we outline PACAP’s involvement and changes in various human pathological conditions.

show abstract

“…We previously demonstrated that NCX2 HET, but not NCX1 HET, displays altered motility in the distal colon (Azuma et al, 2012). In addition, we recently demonstrated that NCX1 HET and NCX2 HET display altered motility in the gastric fundus (Azuma et al, 2016a). Currently, little is known about the potential role of NCX isoforms in small intestinal motility.…”

Section: Introductionmentioning

confidence: 99%

Roles of Na+/Ca2+ exchanger isoforms NCX1 and NCX2 in motility in mouse ileum

Nishiyama

Azuma

Morioka

et al. 2016

Naunyn-Schmiedeberg's Arch Pharmacol

View full text Add to dashboard Cite

Na⁺/Ca²⁺ exchanger‐heterozygote knockout mice display increased relaxation in gastric fundus and accelerated gastric transit in vivo

Abstract: In this study, we demonstrate that NCX1 and NCX2 expressed in neurons regulate the motility in the gastric fundus.

Cited by 14 publications

References 38 publications

Chronic kidney disease after 5/6 nephrectomy disturbs the intestinal microbiota and alters intestinal motility

Chronic kidney disease after 5/6 nephrectomy disturbs the intestinal microbiota and alters intestinal motility

Presence and Effects of Pituitary Adenylate Cyclase Activating Polypeptide Under Physiological and Pathological Conditions in the Stomach

Roles of Na+/Ca2+ exchanger isoforms NCX1 and NCX2 in motility in mouse ileum

Contact Info

Product

Resources

About