Na⁺,K⁺-ATPase activity is increased in rats subjected to chronic administration of ketamine

Abstract

ATPase activity is increased in rats subjected to chronic administration of ketamine.Objective: Schizophrenia is a devastating psychiatric disorder. Symptoms of schizophrenia can be divided into positive, negative and cognitive, and the physiopathology is still been unknown. Na + ,K + -ATPase is a protein in its role as a maintainer of fluid balance in all mammals and alterations in this enzyme could cause brain abnormalities. The aim of our study was to investigate the activity of this enzyme in rats submitted to chronic administration of ketamine. Methods: Adult male Wistar rats were submitted to sub-anaesthetic doses of the 25 mg/kg ketamine by seven consecutive days and the Na + ,K + -ATPase activity was analysed in prefrontal and hippocampus of rats. Results: We observed an increase in Na + ,K + -ATPase activity in prefrontal cortex administration of 25 mg/kg ketamine. However, ketamine has no effect in hippocampus. Conclusion: This evidence indicates that the alteration in Na + ,K + -ATPase may be related with glutamatergic system and consequently could be related to the development of schizophrenia. Significant OutcomesThe pathophysiology of schizophrenia is still poorly understood we observed (a) Na + ,K + -ATPase activity increased in prefrontal cortex after chronic administration of ketamine; (b) no significant change was found in Na + ,K + -ATPase activity in hippocampus; (c) we suggested that chronic administration of 25 mg/kg ketamine provoked imbalance in energetic metabolism by alter the Na + ,K + -ATPase activity in prefrontal cortex. LimitationsThis study presented some limitations, first, few brain areas were assessed; second, advanced techniques would be needed to confirm the imbalance of energetic metabolism provoke by ketamine treatment.

Increased Na+,K+-ATPase activity in the rat brain after meningitis induction byStreptococcus pneumoniae

“…In the neurons, where it may use up to 70% of the cell's total energy consumption, the role of the Na + ,K + ‐ATPase is crucial for basis metabolic requirements and for the specialised functions of nerve impulse transmission (17). Earlier studies have shown that Na + ,K + ‐ATPase activity is altered in ischaemia (18), neurodegenerative diseases (19), with chronic administration of ketamine (20), increased in animal models using lipopolysaccharide (21) and resulted in increased Na + K + pump activity (22) in sepsis also. Furthermore, Na + ,K + ‐ATPase activity was inhibited by interleukin‐1 β (IL‐1 β ) in cardiac myocytes (23) and reduced cerebral cortical cell membrane Na + ,K + ‐ATPase activity in meningitis by Escherichia coli in the newborn piglet (24).…”