Naasia aerilata gen. nov., sp. nov., a member of the family Microbacteriaceae isolated from air

Abstract

Naasia aerilata gen. nov., sp. nov., a member of the family Microbacteriaceae isolated from air A Gram-positive, aerobic, rod-shaped bacterial strain, 5116S-4 T , was isolated from an air sample collected in Suwon city, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that this strain was a new member of the family Microbacteriaceae. The sequence similarities of this strain to the members of the family Microbacteriaceae were less than 97 %, with the highest values observed with Cryobacterium mesophilum MSL-15 T (96.3 %), Cryobacterium roopkundense RuGl7 T (96.2 %), Labedella gwakjiensis KSW2-17 T (96.2 %), Cryobacterium luteum Hh15 T (96.2 %), Cryobacterium psychrophilum DSM 4854 T (96.2 %), Klugiella xanthotipulae 44C3 T (96.0 %) and Amnibacterium kyonggiense KSL51201-037 T (96.0 %). According to the phylogenetic tree, strain 5116S-4 T formed a cluster with A. kyonggiense KSL51201-037 T , Labedella gwakjiensis KSW2-17 T and Lysinimonas soli SGM3-12 T (95.3 % sequence similarity) on the support of high bootstrap values. Cells were motile with single polar flagellum and showed optimum growth at 30 6C and pH 7 without NaCl. Predominant cellular fatty acids were anteiso-C 15 : 0 , iso-C 16 : 0 and anteiso-C 17 : 0 . Polar lipids were diphosphatidylglycerol, dimannosyldiacylglycerol, phosphatidylglycerol, one unknown glycolipid, one unknown phospholipid and one unknown lipid. The acyl type of the cell-wall muramic acid was the acetyl type. Peptidoglycan was supposed to be the type B1 with 2,4-diaminobutyric acid on position 3. Strain 5116S-4 T was clearly distinguishable from the phylogenetically related genera in the family Microbacteriaceae in terms of chemotaxonomic characteristics. On the basis of the chemotaxonomic and phylogenetic characteristics, a novel genus and species are proposed, Naasia aerilata gen. nov., sp. nov. The type strain of Naasia aerilata is 5116S-4 T (5KACC 15517 T 5NBRC 108725 T ).

Glaciihabitans tibetensis gen. nov., sp. nov., a psychrotolerant bacterium of the family Microbacteriaceae, isolated from glacier ice water

“…Amended descriptions of the family were subsequently presented by Stackebrandt et al (1997) and Zhi et al (2009). At the time of writing, the family comprises 39 recognized genera such as Alpinimonas (Schumann et al , 2012), Amnibacterium (Kim & Lee, 2011), Chryseoglobus (Baik et al , 2010), Naasia (Weon et al , 2013), Compostimonas (Kim et al , 2012b), Marisediminicola (Li et al , 2010), Glaciibacter (Katayama et al , 2009), Klugiella (Cook et al , 2008) and Labedella (Lee, 2007). A common feature of the genera within the family Microbacteriaceae is the B-type cell-wall peptidoglycan.…”

Description of Galbitalea soli gen. nov., sp. nov., and Frondihabitans sucicola sp. nov.

“…bacterio.net/classifgenerafamilies.html). Recently, the new genera Alpinimonas, Diaminobutyricimonas, Lysinimonas, Naasia and Pontimonas, isolated from air samples, seawater and silt, have been reported as new members of the family Microbacteriaceae (Jang et al, 2012(Jang et al, , 2013aSchumann et al, 2012;Weon et al, 2013). We isolated two strains, KIS82-1 T and GRS42 T , from soil and from sap of the tree Acer mono, respectively.…”

Aquiluna borgnonia gen. nov., sp. nov., a member of a Microbacteriaceae lineage of freshwater bacteria with small genome sizes

“…Nevertheless, the peptidoglycan structure seemed belonging to the B type, with glycine at the first position, l -2,4-diaminobutyric acid at the third position and structure d -glutamic acid– l -2,4-diaminobutyric acid. This type has so far only found in Compostimonas [23] and Naasia [24].…”