NAC Transcription Factor GmNAC12 Improved Drought Stress Tolerance in Soybean

Yang, Chengfeng; Huang, Yanzhong; Lv, Peiyun; Antwi-Boasiako, Augustine; Begum, Naheeda; Zhao, Tuanjie; Zhao, Jinming

doi:10.3390/ijms231912029

Cited by 18 publications

(6 citation statements)

References 58 publications

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“…Enhancing the expression of ZmNAC111 improved maize drought tolerance [38]. In soybean, GmNAC12overexpressing plants exhibited stronger drought tolerance compared to the wild type [39]. TaNAC5D-2 has a positive effect on the drought tolerance of plants and expressing it in Arabidopsis reduced water loss under drought stress [40].…”

Section: Discussionmentioning

confidence: 99%

The Functions of an NAC Transcription Factor, GhNAC2-A06, in Cotton Response to Drought Stress

Saimi,

Wang,

Liusui

et al. 2023

Plants

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Drought stress imposes severe constraints on crop growth and yield. The NAC transcription factors (TF) play a pivotal role in regulating plant stress responses. However, the biological functions and regulatory mechanisms of many cotton NACs have not been explored. In this study, we report the cloning and characterization of GhNAC2-A06, a gene encoding a typical cotton NAC TF. The expression of GhNAC2-A06 was induced by PEG treatment, drought stress, and ABA treatment. Furthermore, we investigated its function using the virus-induced gene silencing (VIGS) method. GhNAC2-A06 silenced plants exhibited a poorer growth status under drought stress conditions compared to the controls. The GhNAC2-A06 silenced cotton plants had a lower leaf relative water and chlorophyll content and a higher MDA content compared to the controls under the drought treatment. The levels of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) enzyme activity in the GhNAC2-A06 silenced plants were found to be lower compared to the controls when exposed to drought stress. Additionally, the downregulation of the drought stress-related genes, GhSAP12-D07, GhNCED1-A01, GhLEA14-A11, GhZAT10-D02, GhPROT2-A05, GhABF3-A03, GhABF2-D05, GhSAP3-D07, and GhCPK1-D04, was observed in the GhNAC2-A06 silenced cotton. Together, our research reveals that GhNAC2-A06 plays a role in the reaction of cotton to drought stress by affecting the expression of genes related to drought stress. The data obtained from this study lay the theoretical foundation for further in-depth research on the biological function and regulatory mechanisms of GhNAC2-A06.

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Section: Discussionmentioning

confidence: 99%

The Functions of an NAC Transcription Factor, GhNAC2-A06, in Cotton Response to Drought Stress

Saimi,

Wang,

Liusui

et al. 2023

Plants

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“…The use of CRISPR/Cas technology to target transcription factors is a powerful approach for unraveling gene regulation mechanisms, given the central role these proteins play in modulating gene expression. A recent study by Yang et al (2022) showcases the application of CRISPR/Cas to target GmNAC12 that encodes a transcription factor of the NAM/ATAF1/2/CUC2 (NAC) superfamily, to elucidate its function in soybean 22 . In the nonmodel species Fagopyrum tataricum and Scutellaria baicalensis, CRISPR/Cas was employed to mutate the genes encoding two MYB transcription factors FtMYB45 and SbMYB3, respectively.…”

Section: Discussionmentioning

confidence: 99%

Genome editing in almond: A CRISPR-based approach through hairy root transformation

Jedličková,

Štefková,

Sánchez López

et al. 2024

Preprint

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Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas) technology has revolutionized genome manipulation for crop enhancement, providing a powerful toolkit. However, the tissue culture and plant regeneration steps that are critical to the CRISPR/Cas editing framework are often challenging, especially in some woody plant species that exhibit substantial resistance to these procedures. To address this, we have developed an injection-based protocol for inducing hairy roots in almond (Prunus dulcis, syn. Prunus amygdalus), a species known for its recalcitrance to conventional transformation methods. Notably, the hairy root induction method also proved effective in almond x peach hybrids. To evaluate its utility for gene functional analysis, we combined the hairy root transformation system with CRISPR/Cas9 gene editing technology, targeting two transcription factor genes (ERF74 and GAI). Our efforts resulted in transformants with target knock-out, suggesting the potential of this genetic transformation technology as a valuable tool for future routine gene function studies in almond.

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“…edited the soybean transcription factor gene GmNAC12 , which decreased the survival of the transgenic plants exposed to drought stress by at least 12%. They concluded that GmNAC12 is a key gene that positively regulates soybean tolerance to drought conditions ( Yang C.F., et al., 2022 ).…”

Section: Application Of Crispr/cas9 Gene Editing Technology In Soybea...mentioning

confidence: 99%

Advances in CRISPR/Cas9-based research related to soybean [Glycine max (Linn.) Merr] molecular breeding

Yao,

Zhou,

Zhang

et al. 2023

Front. Plant Sci.

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Soybean [Glycine max (Linn.) Merr] is a source of plant-based proteins and an essential oilseed crop and industrial raw material. The increase in the demand for soybeans due to societal changes has coincided with the increase in the breeding of soybean varieties with enhanced traits. Earlier gene editing technologies involved zinc finger nucleases and transcription activator-like effector nucleases, but the third-generation gene editing technology uses clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9). The rapid development of CRISPR/Cas9 technology has made it one of the most effective, straightforward, affordable, and user-friendly technologies for targeted gene editing. This review summarizes the application of CRISPR/Cas9 technology in soybean molecular breeding. More specifically, it provides an overview of the genes that have been targeted, the type of editing that occurs, the mechanism of action, and the efficiency of gene editing. Furthermore, suggestions for enhancing and accelerating the molecular breeding of novel soybean varieties with ideal traits (e.g., high yield, high quality, and durable disease resistance) are included.

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NAC Transcription Factor GmNAC12 Improved Drought Stress Tolerance in Soybean

Cited by 18 publications

References 58 publications

The Functions of an NAC Transcription Factor, GhNAC2-A06, in Cotton Response to Drought Stress

The Functions of an NAC Transcription Factor, GhNAC2-A06, in Cotton Response to Drought Stress

Genome editing in almond: A CRISPR-based approach through hairy root transformation

Advances in CRISPR/Cas9-based research related to soybean [Glycine max (Linn.) Merr] molecular breeding

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