NACE for the analysis of acrylamide in food

Abstract

An NACE method was developed for the quantitative determination of acrylamide in processed food. The method is premised on the modification of the aqueous acid-base character of acrylamide in an organic solution. Acrylamide, which is a polar molecule in aqueous solution, in a low-pH environment in ACN acquires a proton, and thereby migrates under its own electrophoretic mobility in CE. Thus, nonaqueous separation of acrylamide was achieved by employing 30 mmol/L HClO(4) in ACN as the running electrolyte. The detection limit of the method for acrylamide was found as 0.041 mg/L using UV detection at 200 nm. The run-to-run and day-to-day precisions for the corrected peak areas were calculated as 1.65 and 3.90%, respectively. The applicability of the method has been demonstrated by analyzing acrylamide in the samples of potato chips and French fries. The method is simple, rapid, inexpensive, and widely applicable for the determination of acrylamide in food samples.

A Review of Current Methods for the Determination of Acrylamide in Food Products

“…The results showed that the method could be used for quantitative purposes on either food or environmental samples. A nonaqueous CE method was developed for the quantitative determination of AA in processed food like potato chips and French fries (Başkan and Erim 2007). The AA amount of these samples were found to be 2.95±0.11 mg/kg.…”

Review of Research into the Determination of Acrylamide in Foods

“…Capillary electrophoresis (CE) has the characteristics of fast analysis speed and high separation efficiency, and requires a small amount of sample, making it an effective tool for the analysis of trace components in foods [65,66]. CE is based on different charge ratios of the target substance to achieve efficient separation.…”

Determination of acrylamide in potato crisps by capillary electrophoresis with quantum dot‐mediated LIF detection

“…However, the polar AAM does not have strong UV absorption and is electrochemically inactive, giving rise to a detection problem. Although the use of UV cell with extended optical path can improve detection sensitivity, the separation efficiency is greatly reduced [14,15]. The recent availability of economic semiconductor laser makes LIF an affordable solution for the determination of AAM, which requires very high detection sensitivity.…”