Nachweis und Identifizierung von Protein‐Phosphorylierungen in Histidinen mithilfe von HNP‐Korrelationen

Rare-earth phosphate microspheres with unique structures were developed as affinity probes for the selective capture and tagging of phosphopeptides. Prickly REPO(4) (RE = Yb, Gd, Y) monodisperse microspheres, that have hollow structures, low densities, high specific surface areas, and large adsorptive capacities were prepared by an ion-exchange method. The elemental compositions and crystal structures of these affinity probes were confirmed by energy-dispersive spectroscopy (EDS), powder X-ray diffraction (XRD), and Fourier-transform infrared (FTIR) spectroscopy. The morphologies of these compounds were investigated using scanning electron microscopy (SEM), transmission electron microscopy (TEM), and nitrogen-adsorption isotherms. The potential ability of these microspheres for selectively capturing and labeling target biological molecules was evaluated by using protein-digestion analysis and a real sample as well as by comparison with the widely used TiO(2) affinity microspheres. These results show that these porous rare-earth phosphate microspheres are highly promising probes for the rapid purification and recognition of phosphopeptides.

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Monodisperse REPO₄ (RE=Yb, Gd, Y) Hollow Microspheres Covered with Nanothorns as Affinity Probes for Selectively Capturing and Labeling Phosphopeptides

Cheng

Zhang

Liu

et al. 2012

Chemistry A European J

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Nachweis und Identifizierung von Protein‐Phosphorylierungen in Histidinen mithilfe von HNP‐Korrelationen

Cited by 1 publication

References 26 publications

Monodisperse REPO₄ (RE=Yb, Gd, Y) Hollow Microspheres Covered with Nanothorns as Affinity Probes for Selectively Capturing and Labeling Phosphopeptides

Monodisperse REPO₄ (RE=Yb, Gd, Y) Hollow Microspheres Covered with Nanothorns as Affinity Probes for Selectively Capturing and Labeling Phosphopeptides

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Nachweis und Identifizierung von Protein‐Phosphorylierungen in Histidinen mithilfe von HNP‐Korrelationen

Cited by 1 publication

References 26 publications

Monodisperse REPO4 (RE=Yb, Gd, Y) Hollow Microspheres Covered with Nanothorns as Affinity Probes for Selectively Capturing and Labeling Phosphopeptides

Monodisperse REPO4 (RE=Yb, Gd, Y) Hollow Microspheres Covered with Nanothorns as Affinity Probes for Selectively Capturing and Labeling Phosphopeptides

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Monodisperse REPO₄ (RE=Yb, Gd, Y) Hollow Microspheres Covered with Nanothorns as Affinity Probes for Selectively Capturing and Labeling Phosphopeptides

Monodisperse REPO₄ (RE=Yb, Gd, Y) Hollow Microspheres Covered with Nanothorns as Affinity Probes for Selectively Capturing and Labeling Phosphopeptides