NaCl as a physiological modulator of proline metabolism and antioxidant potential in Phyllanthus amarus

Jaleel, Cheruth Abdul; Manivannan, P.; Lakshmanan, Ganapathy Murugan Alagu; Sridharan, R.; Panneerselvam, R.

doi:10.1016/j.crvi.2007.08.009

Cited by 74 publications

(32 citation statements)

References 39 publications

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“…Present results, the antioxidant enzymes peroxidase activity and catalase activity were increased under NaCl salinity ( Figure 6) and further enhanced due to CaCl 2 treatment. These results are in agreement with those reported by (Jaleel et al, 2007). The plants defend against these reactive oxygen species by induction of activities of certain antioxidative enzymes such as catalase, peroxidase (Mittova et al, 2003).…”

Section: Peroxidase and Catalase Activitysupporting

confidence: 83%

Effects of NaCl, CaCl2 and their Combination of Salt on Seed Germination and Seedling Growth of Lycopersicum esculentum L.

Sivasankaramoorthy

2014

ILNS

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To compare the effect of NaCl, CaCl 2 and their combinations on germination and early seedling growth stages of Lycopersicum esculentum L., were studied under pot experiments 2008. Results indicated that significant increases were recorded in percentage of germination, seedling fresh and dry weights, seedling length, water content, catalase activity and photosynthetic pigments (chlorophyll a, b and total chlorophylls as well as carotenoids) under the low level concentration (20 mM) of NaCl or CaCl and their combination (1:1). On other hand increasing salt concentration in nutrient solution caused significant decrease in all of these parameters. The great reduction occurred under high salinity level of NaCl (50 mM). Meanwhile, peroxidase activity increased significantly with increasing salinity levels from 20 mM to 50 mM of both applied salinity types. Besides, peroxidase activity under NaCl salinity showed a marked increase followed by NaCl + CaCl 2 (1:1) and CaCl 2 at 50 mM.

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Section: Peroxidase and Catalase Activitysupporting

confidence: 83%

Effects of NaCl, CaCl2 and their Combination of Salt on Seed Germination and Seedling Growth of Lycopersicum esculentum L.

Sivasankaramoorthy

2014

ILNS

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“…Plants have evolved several enzymatic and non-enzymatic mechanisms to provide protection against the toxic effects of ROS and to maintain cellular redox homeostasis (Ahmad et al, 2012;Jaleel et al, 2007Jaleel et al, , 2009; Kaur et al, 2012;Sabir et al, 2012;Mishra et al, 2014). ROS scavenging enzymes include catalase (CAT), ascorbate peroxidase (APX), superoxide dismutase (SOD), and glutathione reductase (GR; Noctor and Foyer, 1998).…”

Section: Introductionmentioning

confidence: 99%

Cold stress affects antioxidative response and accumulation of medicinally important withanolides in Withania somnifera (L.) Dunal

Mir

Khazir

et al. 2015

Industrial Crops and Products

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Withania somnifera (L.) Dunal (Indian ginseng) is a high value medicinal plant. It synthesizes a large array of biologically active withanolides. In this study, two month old seedlings of AGB002 (wild genotype) and AGB025 (cultivated genotype) of W. somnifera were subjected to cold stress (4 • C) under controlled envi-ronment. Plants were analyzed for three medicinally important secondary metabolites (withanolide A, withanone and withaferin A), lipid peroxidation (MDA), cell injury, superoxide radical (O 2 •− ) accumula-tion and anti-oxidative enzymes activities such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR). Increases in the titers of superoxide anion and MDA were observed from day 1 to day 7 in both genotypes, although the increase on the first day of exposure was significantly higher. Enzymatic activities of SOD, CAT, APX and GR also showed an increasing trend in both genotypes and reached a maximum on day 7 of the cold temperature exposure; however, this increase was higher in AGB002 than AGB025. Withanolide A (WS-1) in the roots of both genotypes sig-nificantly decreased on the first day of cold exposure and then showed a recovery until day 7. WS-1 was not detected in the leaves of either genotype. Withanone (WS-2) content in the leaves also decreased towards the end of the cold period in both genotypes. Cold stress also elicited the accumulation of WS-2 in AGB025, but was not detectable in control seedlings. At maturity, WS-2 was also detected in control plants. Furthermore, a significant increase in the leaf withaferin A (WS-3) content was recorded from day 1 to day 7 of the cold exposure in both the genotypes, suggesting the possible involvement of with-anolides in cold-protection. AGB002 showed comparatively higher accumulation of antioxidant enzymes and selected marker withanolides than AGB025, indicating that AGB002 is better adapted to cold than AGB025. It could be inferred from these observations that cold stress induces bioactive withanolide accu-mulation in W. somnifera as a mechanism for scavenging reactive oxygen species (ROS). These studies also provide an impetus for enhancing the withanolide accumulation in W. somnifera using controlled environment technology.

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“…Potassium phosphate is one of the major salts in the medium for productions of microbial polysaccharides and enzymes as well as a well-known ingredient in buffer solutions [11,16,19]. Sodium chloride was reported to be used as a physiological modulator of biosynthetic pathway of biopolymers [10]. Magnesium sulfate added to medium assisted spore germination and initial growth of A. fisheri, which resulted in 1.9 fold increased production of xylanase [19].…”

Section: Optimization Of Salts Using Response Surface Methodsmentioning

confidence: 99%

Comparison of Statistical Methods for Optimization of Salts in Medium for Production of Carboxymethylcellulase of Bacillus amyloliquefaciens DL-3 by a Recombinant E. coli JM109/DL-3

Lee¹,

Kim²,

Gao³

et al. 2011

Journal of Life Science

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The optimal concentrations of salts in medium for cell growth and the production of carboxymethylcellulase (CMCase) by a recombinant E. coli JM109/DL-3 were established using two statistical methods: orthogonal array method (OAM) and response surface method (RSM). The analysis of variance (ANOVA) of data based on OAM indicated that K2HPO4 gave maximum sum of square (S) and percentage contribution (P) for cell growth as well as production of CMCase. The optimal concentrations of K2HPO4, NaCl, MgSO4•7H2O, and (NH4)2SO4 in medium for cell growth extracted by Qualitek-4 (W32b) Software were 10.0, 1.0, 0.2, and 0.6 g/l, respectively, whereas those for the production of CMCase by E. coli JM109/DL-3 were 5.0, 1.0, 0.4, and 0.6 g/l. The analysis of variance (ANOVA) resulting from RSM indicated that a highly significant salt for cell growth was K2HPO4 ("probe>F" less than 0.0001), whereas K2HPO4 and MgSO4•7H2O were significant for the production of CMCase. The optimal concentrations of K2HPO4, NaCl, MgSO4•7H2O, and (NH4)2SO4 for cell growth extracted by Design Expert Software were 7.44, 1.08, 0.22, and 0.88 g/l, respectively, whereas those for production of CMCase were 5.84, 0.69, 0.28, and 0.54 g/l. The optimal concentrations of salts and their influences on cell growth and production of CMCase extracted by OAM were almost the same as those by RSM. Production of CMCase by a recombinant E. coli JM109/DL-3 under optimized concentration of salts was 1.93 times higher than that by Bacillus amyloliquifaciens DL-3.

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NaCl as a physiological modulator of proline metabolism and antioxidant potential in Phyllanthus amarus

Cited by 74 publications

References 39 publications

Effects of NaCl, CaCl<sub>2</sub> and their Combination of Salt on Seed Germination and Seedling Growth of <i>Lycopersicum esculentum</i> L.

Effects of NaCl, CaCl<sub>2</sub> and their Combination of Salt on Seed Germination and Seedling Growth of <i>Lycopersicum esculentum</i> L.

Cold stress affects antioxidative response and accumulation of medicinally important withanolides in Withania somnifera (L.) Dunal

Comparison of Statistical Methods for Optimization of Salts in Medium for Production of Carboxymethylcellulase of Bacillus amyloliquefaciens DL-3 by a Recombinant E. coli JM109/DL-3

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