NaCl-induced changes of NAD(P) malic enzyme activities in Eucalyptus citriodora leaves

Aragão, Maria Erivalda Farias de; Jolivet, Yves; Lima, M. Guia Silva; Melo, Dirce Fernandes de; Dizengremel, Pierre

doi:10.1007/pl00009699

Cited by 5 publications

(6 citation statements)

References 42 publications

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“…Si treatment of salt-stressed plants has been found to result in accumulation of organic acids in several plant species: Arabidopsis thaliana [62], Oryza sativa [63], Eucalyptus [64] and canola plants [65]. In accordance with the current study, these studies suggest that Si induced accumulation of organic acids and has stress alleviation effects on salt stressed plants.…”

Section: Metabolic Reprogramming Of Sugars and Organic Acids In Si Treated Salt Stressed Tomato Plantssupporting

confidence: 90%

A Global Metabolic Map Defines the Effects of a Si-Based Biostimulant on Tomato Plants under Normal and Saline Conditions

et al. 2021

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The ongoing unpredictability of climate changes is exponentially exerting a negative impact on crop production, further aggravating detrimental abiotic stress effects. Several research studies have been focused on the genetic modification of crop plants to achieve more crop resilience against such stress factors; however, there has been a paradigm shift in modern agriculture focusing on more organic, eco-friendly and long-lasting systems to improve crop yield. As such, extensive research into the use of microbial and nonmicrobial biostimulants has been at the core of agricultural studies to improve crop growth and development, as well as to attain tolerance against several biotic and abiotic stresses. However, the molecular mechanisms underlying the biostimulant activity remain enigmatic. Thus, this study is a liquid chromatography-mass spectrometry (LC-MS)-based untargeted metabolomics approach to unravel the hypothetical biochemical framework underlying effects of a nonmicrobial biostimulant (a silicon-based formulation) on tomato plants (Solanum lycopersium) under salinity stress conditions. This metabolomics study postulates that Si-based biostimulants could alleviate salinity stress in tomato plants through modulation of the primary metabolism involving changes in the tricarboxylic acid cycle, fatty acid and numerous amino acid biosynthesis pathways, with further reprogramming of several secondary metabolism pathways such as the phenylpropanoid pathway, flavonoid biosynthesis pathways including flavone and flavanol biosynthesis. Thus, the postulated hypothetical framework, describing biostimulant-induced metabolic events in tomato plants, provides actionable knowledge necessary for industries and farmers to, confidently and innovatively, explore, design, and fully implement Si-based formulations and strategies into agronomic practices for sustainable agriculture and food production.

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Section: Metabolic Reprogramming Of Sugars and Organic Acids In Si Treated Salt Stressed Tomato Plantssupporting

confidence: 90%

A Global Metabolic Map Defines the Effects of a Si-Based Biostimulant on Tomato Plants under Normal and Saline Conditions

et al. 2021

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“…2002). In Eucalyptus plants, the induction of ME by NaCl has been also described (Farias De Aragao et al . 1997).…”

Section: Discussionmentioning

confidence: 80%

“…Induction of ICDH and G6PDH has been reported in pea and pepper plants subjected to Cd stress (Romero-Puertas et al 1999;León et al 2002), and in Mesembryanthemum crystallinum plants by salt stress (Popova et al 2002). In Eucalyptus plants, the induction of ME by NaCl has been also described (Farias De Aragao et al 1997).…”

Section: Discussionmentioning

confidence: 95%

The dehydrogenase‐mediated recycling of NADPH is a key antioxidant system against salt‐induced oxidative stress in olive plants

Valderrama

Corpas

Carreras

et al. 2006

Plant Cell & Environment

215

146

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NADPH is an important molecule in the redox balance of the cell. In this paper, using olive tissue cultures as a model of the function of the NADPH-generating dehydrogenases in the mechanism of oxidative stress induced by severe salinity conditions was studied. When olive ( Olea europaea ) plants were grown with 200 m M NaCl, a 40% reduction in leaf fresh weight was produced. The content of nonenzymatic antioxidants such as ascorbate and glutathione was diminished between 20% to 39%, whereas the H 2 O 2 content was increased threefold. In contrast, the analysis of the activity and protein contents of the main antioxidative enzymes showed a significant increase of catalase, superoxide dismutase and glutathione reductase. Overall, these changes strongly suggests that NaCl induces oxidative stress in olive plants. On the other hand, while the content of glucose-6-phosphate was increased almost eightfold in leaves of plants grown under salt stress, the content of NAD(P)H (reduced and oxided forms) did not show significant variations. Under salt stress conditions, the activity and protein contents of the main NADPH-recycling enzymes, glucose-6-phosphate dehydrogenase (G6PDH), isocitrate dehydrogenase (ICDH), malic enzyme (ME) and ferrodoxin-NADP reductase (FNR) showed an enhancement of 30-50%. In leaves of olive plants grown with 200 m M NaCl, analysis of G6PDH by immunocytochemistry and confocal laser scanning microscopy showed a general increase of this protein in epidermis, palisade and spongy mesophyll cells. These results indicate that in olive plants, salinity causes reactive oxygen species (ROS)-mediated oxidative stress, and plants respond to this situation by inducing different antioxidative enzymes, especially the NADPH-producing dehydrogenases in order to recycle NADPH necessary for the protection against oxidative damages. These NADP-dehydrogenases appear to be key antioxidative enzymes in olive plants under salt stress conditions.

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“…Numerous studies have shown that the exposure to NaCl stress inhibits nitrate uptake (Word et al 1986;Botella et al 1994;Khan and Srivastava 1998) and results in decreased nitrate content in plants tissues (Hu et Schmidhalter 1998). Nevertheless, results of some investigations showed that nitrate level in plants is not modified by NaCl treatment (Farias de Aragao et al 1997). Our observations are consistent with opinion of Kaiser and Huber (2001) that NR in plants growing in stress conditions can be under strong substrate limitation.…”

Section: Discussionmentioning

confidence: 99%

Effect of salt and water stresses on growth, nitrogen and phosphorus metabolism in Cucumis sativus L. seedlings

et al. 2011

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Plants exposed to osmotic stress exhibit changes in their physiology and metabolism. In general, osmotic stress reduces water availability and causes nutritional imbalance in plants. In the present study, we compared the response of cucumber (Cucumis sativus L. var. W³adko F-1) to ionic (100 mmol·dm -3 NaCl) and osmotic stress (10% PEG 6000). Both stress factors reduced significantly fresh and dry weight of 7-day-old cucumber seedlings. Under PEG treatment reduction of cucumber dry mass was lesser than in fresh mass, whereas under salt stress decrease in dry weight of cucumber shoots was more pronounced than in fresh mass. Salt stress caused severe decrease in nitrate concentration and activity of nitrate reductase (NR). In cotyledons nitrate content declined to 17% of the control and similar reduction in NR activity was observed. In the roots, observed changes were not so drastic but there was also strong interaction between reduction in nitrate content and NR activity. Under 10% PEG both nitrate concentration and NR activity in cucumber roots were significantly higher in comparison to control plants. In cotyledons NR activity was significantly lower than in control plants, while decrease in nitrate content was not statistically significant. Phosphate concentration did not change significantly in cucumber cotyledons but increased in roots treated both NaCl (32% increase) and PEG (53% increase). Similar tendencies were observed in acid phosphatase activity. Obtained results indicated that osmotic and salt stresses evoke differential responses, particularly in growth reduction and nitrogen metabolism in cucumber seedlings.

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NaCl-induced changes of NAD(P) malic enzyme activities in Eucalyptus citriodora leaves

Cited by 5 publications

References 42 publications

A Global Metabolic Map Defines the Effects of a Si-Based Biostimulant on Tomato Plants under Normal and Saline Conditions

A Global Metabolic Map Defines the Effects of a Si-Based Biostimulant on Tomato Plants under Normal and Saline Conditions

The dehydrogenase‐mediated recycling of NADPH is a key antioxidant system against salt‐induced oxidative stress in olive plants

Effect of salt and water stresses on growth, nitrogen and phosphorus metabolism in Cucumis sativus L. seedlings

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