NaCl tolerance in Lycopersicon pennellii introgression lines: QTL related to physiological responses

Frary, Anne; Keleş, Davut; Pınar, Hasan; Göl, Deniz; Doğanlar, Sami

doi:10.1007/s10535-011-0111-x

Cited by 19 publications

(8 citation statements)

References 24 publications

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“…With a similarly promising performance, the novel rootstocks 'HUJ1' and 'HUJ2' that are based on the S. pennellii introgression lines (ILs), are considered to maintain stable growth when exposed to salt stress [25]. When stressed, the ILs performed significantly better at maintaining plant growth than the salt-tolerant wild species, as well as parental line LA716.…”

Section: Impact Of Different Rootstocks On Scion Performancementioning

confidence: 99%

“…Although 'Beaufort' is proving to be an efficient rootstock, newly developed cultivars are being hybridized as rootstock that may eventually exceed this performance. For example, novel rootstock cultivars have been obtained by crossing with the wild tomato species Solanum pennellii accession LA716, which is considered to be more tolerant in stress conditions than related tomato species S. lycopersicum [25].…”

Section: Introductionmentioning

confidence: 99%

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Novel S. pennellii × S. lycopersicum Hybrid Rootstocks for Tomato Production with Reduced Water and Nutrient Supply

et al. 2021

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Drought stress and nutrient deficiency are limiting factors in vegetable production that will have a decisive role due to the challenges of climate change in the future. The negative effects of these stressors on yield can be mitigated by crop grafting. The increasing demands for resource-use efficiency in crop production, therefore, require the development and phenotyping of more resilient rootstocks, and the selection of appropriate scions. We tested the effect of combined drought stress and nutrient deficiency on yield and fruit quality of the two tomato cultivars ‘Lyterno’ and ‘Tastery’ in the greenhouse, grafted onto different rootstock genotypes. The use of four different rootstocks, including two novel S. pennellii × S. lycopersicum hybrids and the proven-effective use of ‘Beaufort’, as well as self-grafted plants, allowed conclusions to be drawn about the differential stress mitigation of the rootstocks used. The stress-induced yield reduction of the scion ‘Lyterno’ can be mitigated more significantly by the novel hybrid rootstocks than by the commercial rootstock ‘Beaufort’. At the same time, however, the individual fruit weight and the lycopene content of the fruits were significantly reduced when grafted onto the hybrid rootstocks. In contrast, the cultivar ‘Tastery’ showed a weak stress response, so that a generally positive influence of the rootstocks independently of the scions could not be demonstrated. We conclude that, particularly for more sensitive cultivars, the selection of more resilient rootstocks offers the potential for sustainable and resource-efficient production not competing with the overall quality of tomatoes.

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Section: Impact Of Different Rootstocks On Scion Performancementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Novel S. pennellii × S. lycopersicum Hybrid Rootstocks for Tomato Production with Reduced Water and Nutrient Supply

et al. 2021

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“…To specifically target stress-related mechanisms, we identified 389 potential stress-related genes by mining the literature (Supplementary Table 22). These genes were filtered against drought-or salt-related QTLs detected in the ILs [14][15][16][17][18][19] , which resulted in the identification of 100 candidate genes ( Fig. 3 and Supplementary Note).…”

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confidence: 99%

The genome of the stress-tolerant wild tomato species Solanum pennellii

et al. 2014

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Solanum pennellii is a wild tomato species endemic to Andean regions in South America, where it has evolved to thrive in arid habitats. Because of its extreme stress tolerance and unusual morphology, it is an important donor of germplasm for the cultivated tomato Solanum lycopersicum 1 . Introgression lines (ILs) in which large genomic regions of S. lycopersicum are replaced with the corresponding segments from S. pennellii can show remarkably superior agronomic performance 2 . Here we describe a high-quality genome assembly of the parents of the IL population. By anchoring the S. pennellii genome to the genetic map, we define candidate genes for stress tolerance and provide evidence that transposable elements had a role in the evolution of these traits. Our work paves a path toward further tomato improvement and for deciphering the mechanisms underlying the myriad other agronomic traits that can be improved with S. pennellii germplasm.Crosses between distantly related plants can lead to substantial improvements in performance. Notably, S. pennellii × S. lycopersicum ILs have been used to define numerous quantitative trait loci (QTLs) for superior yield, chemical composition, morphology, abiotic stress tolerance and extreme heterosis 3,4 . Although genetic studies have proven informative, few genes underlying specific QTLs have been cloned, largely because of the lack of a S. pennellii genome sequence. To support QTL analyses, we sequenced the genome of S. pennellii using Illumina sequencing with ~190-fold coverage ( Fig. 1 and Supplementary Tables 1-5). The initial assembly size was 942 Mb, with a scaffold N50 value of 1.7 Mb and N90 value of 0.43 Mb (Table 1 and Supplementary Tables 6 and 7). We estimated the total genome size to be about 1.2 Gb using a k-mer-based analysis ( Supplementary Fig. 1 and Supplementary Table 8), in accordance with previous estimations 3,4 . We anchored 97.1% of the genome assembly to chromosomes using genetic maps and restriction site-associated DNA sequencing (RAD-seq)-based markers from the IL population 5 (Supplementary Note). Comparison of the assembly to publicly available BAC sequences indicated an accuracy of >99.9%, and a satisfactory accuracy of gap-filled regions was shown by realigning reads (Supplementary Fig. 2 and Supplementary Table 9). Of the 307,350 S. lycopersicum and 7,812 S. pennellii publicly available ESTs, 93% and >96% could be aligned to the genome, respectively (Supplementary Table 10), indicating comprehensive coverage of the gene-rich regions. We predicted 32,273 high-confidence genes and a potential set of 44,966 protein-coding genes and checked these

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“…The increase in Na + concentration causes a decrease in K + , Ca 2+ , Mg 2+ which inhibits growth [58]. The potassium content decreased in the three lines under salt stress, this has been also observed in tomato by Frary et al [25]. Salt stress causes a potassium deficiency in plants, which can be explained by the competition between Na + and K + for the same transport system at the root surface [8,54].…”

Section: Discussionmentioning

confidence: 56%

Comparison of the responses to NaCl stress of three tomato introgression lines

Rebah

Ouhibi

Alamer

et al. 2018

Acta Biologica Hungarica

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We aimed to examine the response of three tomato introgression lines (IL925.3, IL925.5 and IL925.6) to NaCl stress. These lines originated from a cross between M82 (Solanum lycopersicum) and the wild salttolerant tomato Solanum pennellii, each line containing a different fragment of the S.pennellii genome. Salt-sensitive phenotypes related to plant growth and physiology, and the response of antioxidants, pigments and antioxidant enzymes were measured. In general, salt stress decreased the fresh weight of leaves, leaf area and leaf number and an increase of Na + accumulation in aerial parts was observed, which caused a reduction in the absorption of K + and Ca 2+. Salt stress also induced a decrease in chlorophyll, carotenoids and lipid peroxidation (MDA) and an increase in anthocyanins and reduced ascorbate, although some differences were seen between the lines, for example for carotenoid levels. Guaiacol peroxidase, catalase and glutathione reductase activity enhanced in aerial parts of the lines, but again some differences were seen between the three lines. It is concluded that IL925.5 might be the most sensitive line to salt stress as its dry weight loss was the greatest in response to salt and this line showed the highest Na + ion accumulation in leaves.

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NaCl tolerance in Lycopersicon pennellii introgression lines: QTL related to physiological responses

Cited by 19 publications

References 24 publications

Novel S. pennellii × S. lycopersicum Hybrid Rootstocks for Tomato Production with Reduced Water and Nutrient Supply

Novel S. pennellii × S. lycopersicum Hybrid Rootstocks for Tomato Production with Reduced Water and Nutrient Supply

The genome of the stress-tolerant wild tomato species Solanum pennellii

Comparison of the responses to NaCl stress of three tomato introgression lines

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