NaCl transport and ultrastructure of opercular epithelium from a freshwater‐adapted euryhaline teleost, Fundulus heteroclitus

Marshall, W. S.; Bryson, S.E.; Darling, P.; Whitten, C.; Patrick, Marjorie L.; Wilkie, Michael P.; Wood, Chris M.; Buckland‐Nicks, J.

doi:10.1002/(sici)1097-010x(19970101)277:1<23::aid-jez3>3.3.co;2-j

Cited by 15 publications

(15 citation statements)

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“…In some teleosts, the epithelium of the operculum contributes substantially to ionoregulation (Marshall, 1995). Similar to branchial epithelia, the opercular membrane contains a high density of chloride cells (Karnaky et al, 1984;Kültz, 1995;Marshall et al, 1997). Opercular chloride cells have been used in many studies on chloride cell functions as a model for branchial chloride cells.…”

Section: Discussionmentioning

confidence: 99%

Excellent Salinity Tolerance of Mozambique Tilapia (Oreochromis mossambicus): Elevated Chloride Cell Activity in the Branchial and Opercular Epithelia of the Fish Adapted to Concentrated Seawater

et al. 2000

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Section: Discussionmentioning

confidence: 99%

Excellent Salinity Tolerance of Mozambique Tilapia (Oreochromis mossambicus): Elevated Chloride Cell Activity in the Branchial and Opercular Epithelia of the Fish Adapted to Concentrated Seawater

et al. 2000

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“…The killifish opercular membrane is a popular model for electrophysiology because the tissue contains a rich and dense population of ionocytes (100,101). Branchial and opercular ionocytes of killifish become larger in FW than in SW (81,103), opposite in expression pattern to that seen in other teleosts including tilapia and salmonids (54,58). Branchial ionocytes of SW-acclimated killifish showed the typical localization pattern for type-IV ionocytes of tilapia, basolateral NKA, and NKCC1 and apical CFTR ( Fig.…”

Section: Furukawa and T Kaneko Personal Communication)mentioning

confidence: 94%

“…Second, the size of gill ionocytes increases greatly after transfer from SW to FW in killifish (81,103), but ionocytes enlarge after transfer from FW to SW in other teleosts including salmonids and tilapia (54,58). This may be a reflection of the historical SW origin of this killifish species (F. heteroclitus), which lives along the coast of Atlantic Ocean (Fig.…”

Section: What Is the Cause Of Such Diversity?mentioning

confidence: 99%

Diverse mechanisms for body fluid regulation in teleost fishes

Takei

Hiroi

Takahashi

et al. 2014

American Journal of Physiology-Regulatory, Integrative and Comp

115

109

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Teleost fishes are the major group of ray-finned fishes and represent more than one-half of the total number of vertebrate species. They have experienced in their evolution an additional third-round whole genome duplication just after the divergence of their lineage, which endowed them with an extra adaptability to invade various aquatic habitats. Thus their physiology is also extremely diverse compared with other vertebrate groups as exemplified by the many patterns of body fluid regulation or osmoregulation. The key osmoregulatory organ for teleosts, whose body fluid composition is similar to mammals, is the gill, where ions are absorbed from or excreted into surrounding waters of various salinities against concentration gradients. It has been shown that the underlying molecular physiology of gill ionocytes responsible for ion regulation is highly variable among species. This variability is also seen in the endocrine control of osmoregulation where some hormones have distinct effects on body fluid regulation in different teleost species. A typical example is atrial natriuretic peptide (ANP); ANP is secreted in response to increased blood volume and acts on various osmoregulatory organs to restore volume in rainbow trout as it does in mammals, but it is secreted in response to increased plasma osmolality, and specifically decreases NaCl, and not water, in the body of eels. The distinct actions of other osmoregulatory hormones such as growth hormone, prolactin, angiotensin II, and vasotocin among teleost species are also evident. We hypothesized that such diversity of ionocytes and hormone actions among species stems from their intrinsic differences in body fluid regulation that originated from their native habitats, either fresh water or seawater. In this review, we summarized remarkable differences in body fluid regulation and its endocrine control among teleost species, although the number of species is still limited to substantiate the hypothesis.

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“…Electrophysiology studies investigating NaCl transport functions of teleost fish gill are numerous (see review by Marshall and Bellamy, 2010), notably on seawater fish. The euryhaline killifish Fundulus heteroclitus has proved to be an excellent model system for the study of ion secretion processes and their endocrine and neural control (Karnaky et al, 1976;Foskett and Scheffey, 1982;Marshall et al, 1997;Marshall and Bellamy, 2010). The NaCl secretion mechanism by marine teleost fish gill is therefore well established.…”

Section: Introductionmentioning

confidence: 99%

Nitric oxide inhibition of NaCl secretion in the opercular epithelium of seawater-acclimated killifish, Fundulus heteroclitus

Gerber

Frank

Madsen

et al. 2016

Journal of Experimental Biology

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Nitric oxide (NO) modulates epithelial ion transport pathways in mammals, but this remains largely unexamined in fish. We explored the involvement of NO in controlling NaCl secretion by the opercular epithelium of seawater killifish using an Ussing chamber approach. Pharmacological agents were used to explore the mechanism(s) triggering NO action. A modified Biotin-switch technique was used to investigate S-nitrosation of proteins. Stimulation of endogenous NO production via the nitric oxide synthase (NOS) substrate l-arginine (2.0 mmol l), and addition of exogenous NO via the NO donor SNAP (10 to 10 mol l), decreased the epithelial short-circuit current (I). Inhibition of endogenous NO production by the NOS inhibitor l-NAME (10 mol l) increased I and revealed a tonic control of ion transport by NO in unstimulated opercular epithelia. The NO scavenger PTIO (10 mol l) supressed the NO-mediated decrease in I, and confirmed that the effect observed was elicited by release of NO. The effect of SNAP on I was abolished by inhibitors of the soluble guanylyl cyclase (sGC), ODQ (10 mol l) and Methylene Blue (10 mol l), revealing NO signalling via the sGC/cGMP pathway. Incubation of opercular epithelium and gill tissues with SNAP (10 mol l) led to S-nitrosation of proteins, including Na/K-ATPase. Blocking of NOS with l-NAME (10 mol l) or scavenging of NO with PTIO during hypotonic shock suggested an involvement of NO in the hypotonic-mediated decrease in I Yohimbine (10 mol l), an inhibitor of α-adrenoceptors, did not block NO effects, suggesting that NO is not involved in the α-adrenergic control of NaCl secretion.

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NaCl transport and ultrastructure of opercular epithelium from a freshwater‐adapted euryhaline teleost, Fundulus heteroclitus

Cited by 15 publications

References 0 publications

Excellent Salinity Tolerance of Mozambique Tilapia (Oreochromis mossambicus): Elevated Chloride Cell Activity in the Branchial and Opercular Epithelia of the Fish Adapted to Concentrated Seawater

Excellent Salinity Tolerance of Mozambique Tilapia (Oreochromis mossambicus): Elevated Chloride Cell Activity in the Branchial and Opercular Epithelia of the Fish Adapted to Concentrated Seawater

Diverse mechanisms for body fluid regulation in teleost fishes

Nitric oxide inhibition of NaCl secretion in the opercular epithelium of seawater-acclimated killifish, Fundulus heteroclitus

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