Nacre-driven water-soluble factors promote wound healing of the deep burn porcine skin by recovering angiogenesis and fibroblast function

Abstract

To assess the recovery effect of water-soluble components of nacre on wound healing of burns, water-soluble nacre (WSN) was obtained from powdered nacre. Alterations to WSN-mediated wound healing characteristics were examined in porcine skin with deep second-degree burns; porcine skin was used as a proxy for human. When WSN was applied to a burned area, the burn-induced granulation sites were rapidly filled with collagen, and the damaged dermis and epidermis were restored to the appearance of normal skin. WSN enhanced wound healing recovery properties for burn-induced apoptotic and necrotic cellular damage and spurred angiogenesis. Additionally, WSN-treated murine fibroblast NIH3T3 cells showed increased proliferation and collagen synthesis. Collectively, the findings indicate that WSN improves the process of wound healing in burns by expeditiously restoring angiogenesis and fibroblast activity. WSN may be useful as a therapeutic agent, with superior biocompatibility to powdered nacre, and evoking less discomfort when applied to a wounded area.

Shell Extracts from the Marine Bivalve Pecten maximus Regulate the Synthesis of Extracellular Matrix in Primary Cultured Human Skin Fibroblasts

“…Our observations are consistent with several studies performed in rats and pig in the presence of shellfish extracts [26], [29], [30], [48]. Pearl extracts from the pearl oyster Pteria martensii placed on an injury on pig skin causes an increase in the granulation tissue, a removal of necrotic tissue, and an enhancement in the synthesis of type I collagen by fibroblasts at the repair site [48]. Moreover, this shell extract increased the expression at the protein and transcriptional level of collagen type I by a fibroblast cell line (NIH-3T3) in vitro [48].…”

“…Furthermore, they are associated with proteins and form proteoglycans such as decorin or versican, which are PGs interacting with collagen type I, to establish a molecular network allowing the ECM assembly [52]. Our observations are consistent with several studies performed in rats and pig in the presence of shellfish extracts [26], [29], [30], [48]. Pearl extracts from the pearl oyster Pteria martensii placed on an injury on pig skin causes an increase in the granulation tissue, a removal of necrotic tissue, and an enhancement in the synthesis of type I collagen by fibroblasts at the repair site [48].…”

“…Moreover, the AIM and the WSM conditions produced no effects on the metabolic activity or on the cell density. In the literature, studies have already demonstrated that such components affect the metabolism or proliferation of vertebrate cells maintained in vitro [25], [30], [46]–[48]. In particular, experiments performed on human dermal fibroblasts demonstrated that the ASM from the shell of the Japanese scallop Patinopecten yessoensis induces an increase in the metabolic activity of these cells after incubation in the presence of extract [30].…”

“…Pearl extracts from the pearl oyster Pteria martensii placed on an injury on pig skin causes an increase in the granulation tissue, a removal of necrotic tissue, and an enhancement in the synthesis of type I collagen by fibroblasts at the repair site [48]. Moreover, this shell extract increased the expression at the protein and transcriptional level of collagen type I by a fibroblast cell line (NIH-3T3) in vitro [48]. All these results are in agreement with those obtained in our study and clearly suggest that shell extracts from Pecten maximus may have a possible involvement in tissue repair or in processes of tissue regeneration of both the dermis and epidermis.…”

Marine shells: Potential opportunities for extraction of functional and health-promoting materials

“…WSN enhanced the wound healing recovery properties for burn-induced apoptotic and necrotic cellular damage and spurred angiogenesis. Additionally, WSN-treated murine fibroblast NIH3T3 cells showed increased proliferation and collagen synthesis [255].…”