NAD-dependent malate dehydrogenase protects against oxidative damage in Escherichia coli K-12 through the action of oxaloacetate

Oh, Tae Jeong; Kim, In Gyu; Park, Seon-Young; Kim, Kug Chan; Shim, Hye Won

doi:10.1016/s1382-6689(01)00093-x

Cited by 38 publications

(20 citation statements)

References 25 publications

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“…In gram-negative bacteria, this protein generally forms a dimeric molecule and participates in the tricarboxylic acid (TCA) cycle. In addition, this protein can protect against oxidative stress via a reductive TCA cycle [34]. Thus, malate dehydrogenase plays an important role in helping the host resist acid and oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Acid-resistant genes of oral plaque microbiome from the functional metagenomics

Zhang

Zheng

et al. 2018

Journal of Oral Microbiology

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Acid resistance is one of key properties assisting the survival of cariogenic bacteria in a dental caries environment, but only a few genes conferring acid resistance have been identified to data. Functional metagenomics provides a systematic method for investigating commensal DNA to identify genes that encode target functions. Here, the host strain Escherichia coli DH10B and a constructed bidirectional transcription vector pSKII+-lacZ contributed to the construction of a metagenomic library, and 46.6 Mb of metagenomic DNA was cloned from carious supragingival plaque of 8children along with screening for lethal functionality. The screen identified 2 positive clones that exhibited a similar aciduric phenotype to that of the positive controls. Bioinformatic analysis revealed that these two genes encoded an ATP/GTP-binding protein and a malate dehydrogenase. Moreover, we also performed functional screening of Streptococcus mutans, since it is one of the predominant cariogenic strains but was not identified in our initial screening. Five positive clones were retrieved. In conclusion, our improved functional metagenomics screening method helped in the identification of important acid resistance genes, thereby providing new insights into the mechanism underlying caries formation as well as in the prevention and treatment of early childhood caries (ECC).

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Section: Discussionmentioning

confidence: 99%

Acid-resistant genes of oral plaque microbiome from the functional metagenomics

Zhang

Zheng

et al. 2018

Journal of Oral Microbiology

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“…Oxidative stress and oxidative damage are considered to play a role in the early stages of the pathophysiological processes of specific cancers, cardiovascular diseases and several neurodegenerative diseases (40). ROS including hydrogen peroxide and hydroxyl radical can be generated by ionizing radiation and has the potential to induce diseases (41). Oxidative stress can be prevented by the action of enzymatic and chemical antioxidant defenses.…”

Section: Discussionmentioning

confidence: 99%

Ebselen Protects Against Oxidative and Morphological Effects of High Concentration Chronic Toluene Exposure on Rat Sciatic Nerves

et al. 2006

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Aim: Neuroprotective effects of ebselen against free radical damage have been studied extensively. Toluene generates reactive oxygen species (ROS) and the toxic effects relating to these reactants. The aim of this study was designed to investigate the effects of chronic toluene inhalation in high concentration on lipid peroxidation, antioxidant enzyme activities and ultrastructural changes in the sciatic nerves of rats.Methods: We also examined the protective effects of ebselen against toluen exposure. Male Wistar albino rats (150-250 g) were divided in three experimental groups: the control, toluene and toluene+ebselen treated group (n=10 for each). Toluene treatment was performed by inhalation of 3000 ppm toluene, in a 8 hr/day and 6 day/week order for 16 weeks. Control group received 1ml serum physiologic and ebselen was given i.p. (10 mg/kg) to toluene+ebselen treated group just after toluene exposure per day. Blood and tissue samples were obtained for biochemical and histopathological investigation. The blood and sciatic nerves were assayed for toluene by gas chromatography.Results: Toluene significantly increased blood and tissue malondialdehyde (MDA), and decreased tissue superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), but not tissue catalase (CAT) levels when compared with controls. Ebselen administration prevented to increase of blood and tissue MDA and decrease to SOD and GSH-Px levels induced by toluene inhalation. Electron micrographs of sciatic nerve in the toluene group shows myelin destructions with onion-bulb and bubble form protrusion on the myelin sheath and axolemma border of myelinated axons. Furthermore, ebselen salvaged the nerve fibers from toluene exposure.Conclusion: Ebselen treatment may provide neuroprotection against toluene neurotoxicity by directly scavenging ROS and by indirectly augmenting their antioxidant capacity.

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“…MDH from Escherichia coli grown under anaerobic conditions participates in a reductive TCA cycle (converting oxaloacetate to malate) that produces succinic acid (Wu et al, 2007). Moreover, it participates in the protection against oxidative stress because oxaloacetate binds to free radicals (Oh et al, 2002). MDH, malic enzymes, and pyruvate carboxylase from Pseudomonas fluorescens are involved in an anti-oxidative pathway converting NADH, a pro-oxidant, into NADPH, an anti-oxidant (Singh et al, 2008).…”

Section: Function Of Malate Dehydrogenasementioning

confidence: 99%

Function, kinetic properties, crystallization, and regulation of microbial malate dehydrogenase

Takahashi-Íñiguez

Aburto-Rodríguez

Vilchis-González

et al. 2016

J. Zhejiang Univ. Sci. B

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Abstract:Malate dehydrogenase (MDH) is an enzyme widely distributed among living organisms and is a key protein in the central oxidative pathway. It catalyzes the interconversion between malate and oxaloacetate using NAD + or NADP + as a cofactor. Surprisingly, this enzyme has been extensively studied in eukaryotes but there are few reports about this enzyme in prokaryotes. It is necessary to review the relevant information to gain a better understanding of the function of this enzyme. Our review of the data generated from studies in bacteria shows much diversity in their molecular properties, including weight, oligomeric states, cofactor and substrate binding affinities, as well as differences in the direction of the enzymatic reaction. Furthermore, due to the importance of its function, the transcription and activity of this enzyme are rigorously regulated. Crystal structures of MDH from different bacterial sources led to the identification of the regions involved in substrate and cofactor binding and the residues important for the dimer-dimer interface. This structural information allows one to make direct modifications to improve the enzyme catalysis by increasing its activity, cofactor binding capacity, substrate specificity, and thermostability. A comparative analysis of the phylogenetic reconstruction of MDH reveals interesting facts about its evolutionary history, dividing this superfamily of proteins into two principle clades and establishing relationships between MDHs from different cellular compartments from archaea, bacteria, and eukaryotes.

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NAD-dependent malate dehydrogenase protects against oxidative damage in Escherichia coli K-12 through the action of oxaloacetate

Cited by 38 publications

References 25 publications

Acid-resistant genes of oral plaque microbiome from the functional metagenomics

Acid-resistant genes of oral plaque microbiome from the functional metagenomics

Ebselen Protects Against Oxidative and Morphological Effects of High Concentration Chronic Toluene Exposure on Rat Sciatic Nerves

Function, kinetic properties, crystallization, and regulation of microbial malate dehydrogenase

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