NAD+ mediated photoelectrochemical biosensor for histone deacetylase Sirt1 detection based on CuO-BiVO4-AgNCs heterojunction and hybridization chain reaction amplification

Gao, Lanlan; Zhou, Yunlei; Cao, Lulu; Hu, Zhenyong; Mao, Xinyue; Zhang, Haowei; Zhang, Miao; Yin, Huanshun; Ai, Shiyun

doi:10.1016/j.aca.2023.341989

Cited by 1 publication

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“…Figure G shows a typical high-resolution spectrum of Bi. The binding energies of Bi 4f 5/2 and Bi 4f 5/2 in Bi 2 MoO 6 alone were 164.52 and 159.18 eV, respectively, indicating that Bi existed in the form of Bi 3+ . For Bi 2 S 3 /Bi 2 MoO 6 , the binding energy of Bi 4f decreased, which might be caused by the formation of heterojunctions, resulting in strong interaction and partial electron transfer between Bi 2 S 3 and Bi 2 MoO 6 .…”

Section: Resultsmentioning

confidence: 99%

Enzymatically Mediated In Situ Generation of Z-Scheme Bi₂S₃/Bi₂MoO₆ Heterojunction-Based Organic Photoelectrochemical Transistor for METTL3/METTL14 Detection

Zhang,

Zhou

et al. 2024

Anal. Chem.

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The OPECT biosensing platform, which connects optoelectronics and biological systems, offers significant amplification and more possibilities for research in biological applications. In this work, a homogeneous organic photoelectrochemical transistor (OPECT) biosensor based on a Bi 2 S 3 /Bi 2 MoO 6 heterojunction was constructed to detect METTL3/METTL14 protein activity. The METTL3/METTL14 complex enzyme was used to catalyze adenine (A) on an RNA strand to m 6 A, protecting m 6 A-RNA from being cleaved by an E. coli toxin (MazF). Alkaline phosphatase (ALP) catalyzed the conversion of Na 3 SPO 3 to H 2 S through an enzymatic reaction. Due to the adoption of the strategy of no fixation on the electrode, the generated H 2 S was easy to diffuse to the surface of the ITO electrode. The Bi 2 S 3 /Bi 2 MoO 6 heterojunction was formed in situ through a chemical replacement reaction with Bi 2 MoO 6 , improving photoelectric conversion efficiency and realizing signal amplification. Based on this "signal on" mode, METTL3/METTL14 exhibited a wide linear range (0.00001−25 ng/μL) between protein concentration and photocurrent intensity with a limit of detection (LOD) of 7.8 fg/μL under optimal experimental conditions. The applicability of the developed method was evaluated by investigating the effect of four plasticizers on the activity of the METTL3/METTL14 protein, and the molecular modeling technique was employed to investigate the interaction between plasticizers and the protein.

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Section: Resultsmentioning

confidence: 99%

Enzymatically Mediated In Situ Generation of Z-Scheme Bi₂S₃/Bi₂MoO₆ Heterojunction-Based Organic Photoelectrochemical Transistor for METTL3/METTL14 Detection

Zhang,

Zhou

et al. 2024

Anal. Chem.

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NAD+ mediated photoelectrochemical biosensor for histone deacetylase Sirt1 detection based on CuO-BiVO4-AgNCs heterojunction and hybridization chain reaction amplification

Cited by 1 publication

References 37 publications

Enzymatically Mediated In Situ Generation of Z-Scheme Bi₂S₃/Bi₂MoO₆ Heterojunction-Based Organic Photoelectrochemical Transistor for METTL3/METTL14 Detection

Enzymatically Mediated In Situ Generation of Z-Scheme Bi₂S₃/Bi₂MoO₆ Heterojunction-Based Organic Photoelectrochemical Transistor for METTL3/METTL14 Detection

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NAD+ mediated photoelectrochemical biosensor for histone deacetylase Sirt1 detection based on CuO-BiVO4-AgNCs heterojunction and hybridization chain reaction amplification

Cited by 1 publication

References 37 publications

Enzymatically Mediated In Situ Generation of Z-Scheme Bi2S3/Bi2MoO6 Heterojunction-Based Organic Photoelectrochemical Transistor for METTL3/METTL14 Detection

Enzymatically Mediated In Situ Generation of Z-Scheme Bi2S3/Bi2MoO6 Heterojunction-Based Organic Photoelectrochemical Transistor for METTL3/METTL14 Detection

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Enzymatically Mediated In Situ Generation of Z-Scheme Bi₂S₃/Bi₂MoO₆ Heterojunction-Based Organic Photoelectrochemical Transistor for METTL3/METTL14 Detection

Enzymatically Mediated In Situ Generation of Z-Scheme Bi₂S₃/Bi₂MoO₆ Heterojunction-Based Organic Photoelectrochemical Transistor for METTL3/METTL14 Detection