NADH spectrofluorometry of rat skin.

Pappajohn, Douglas J.; Penneys, Raymond; Chance, Britton

doi:10.1152/jappl.1972.33.5.684

Cited by 19 publications

(9 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…NADH is an intrinsically fluorescent molecule, which allows one to monitor NADH fluorescence to dynamically interpret the metabolic activity in vivo (Chance et al, 1962;Pappajohn et al, 1972). NADH images of the heart in live zebrafish embryos were collected with a purpose-built multiphoton imaging system (LOCI at the University of Wisconsin, Madison) (Bird et al, 2004;Bird et al, 2005).…”

Section: Multiphoton Fluorescence Imagingmentioning

confidence: 99%

Lrrc10 is required for early heart development and function in zebrafish

Kim

Antkiewicz

Long

et al. 2007

Developmental Biology

View full text Add to dashboard Cite

Leucine-rich Repeat Containing protein 10 (LRRC10) has recently been identified as a cardiac-specific factor in mice. However, the function of this factor remains to be elucidated. In this study, we investigated the developmental roles of Lrrc10 using zebrafish as an animal model. Knockdown of Lrrc10 in zebrafish embryos (morphants) using morpholinos caused severe cardiac morphogenic defects including a cardiac looping failure accompanied by a large pericardial edema, and embryonic lethality between day 6 and 7 post fertilization. The Lrrc10 morphants exhibited cardiac functional defects as evidenced by a decrease in ejection fraction and cardiac output. Further investigations into the underlying mechanisms of the cardiac defects revealed that the number of cardiomyocyte was reduced in the morphants. Expression of two cardiac genes was deregulated in the morphants including an increase in atrial natriuretic factor, a hallmark for cardiac hypertrophy and failure, and a decrease in cardiac myosin light chain 2, an essential protein for cardiac contractility in zebrafish. Moreover, a reduced fluorescence intensity from NADH in the morphant heart was observed in live zebrafish embryos as compared to control. Taken together, the present study demonstrates that Lrrc10 is necessary for normal cardiac development and cardiac function in zebrafish embryos, which will enhance our understanding of congenital heart defects and heart disease.

show abstract

Section: Multiphoton Fluorescence Imagingmentioning

confidence: 99%

Lrrc10 is required for early heart development and function in zebrafish

Kim

Antkiewicz

Long

et al. 2007

Developmental Biology

View full text Add to dashboard Cite

show abstract

“…In 1962, Chance et al exploited this phenomenon to show that microfluorometry of NADH in intact cells and tissues gives a continuous measurement of intracellular oxidation-reduction states in vivo (1). This pioneering work served as a precursor for a variety of studies that used direct monitoring of NADH fluorescence to dynamically interpret the metabolic activity within the cell (2,3). An intuitive application of this technique is to study carcinogenesis in a variety of organ sites such as the breast, which are known to have increased metabolic rates (4).…”

Section: Introductionmentioning

confidence: 99%

Metabolic Mapping of MCF10A Human Breast Cells via Multiphoton Fluorescence Lifetime Imaging of the Coenzyme NADH

Bird

Long²,

Vrotsos³

et al. 2005

Cancer Research

353

373

View full text Add to dashboard Cite

Biochemical estimation of NADH concentration is a useful method for monitoring cellular metabolism, because the NADH/NAD + reduction-oxidation pair is crucial for electron transfer in the mitochondrial electron chain. In this article, we present a novel method for deriving functional maps of intracellular reduction-oxidation ratio in vivo via measurement of the fluorescence lifetimes and the ratio of free and protein-bound NADH using two-photon fluorescence lifetime imaging (FLIM). Through systematic analysis of FLIM data from the control cells, it was observed that there is a statistically significant decrease in the fluorescence lifetime of both free and protein-bound NADH and the contribution of proteinbound NADH as cells progress from an early to logarithmic to confluent phase. Potassium cyanide (KCN) treatment and serum starvation of cells yielded similar changes. There was a statistically significant decrease in the fluorescence lifetime of protein-bound and free NADH at the early and logarithmic phase of the growth curve and a statistically significant decrease in the contribution of protein-bound NADH relative to that observed in the control cells at all three phases of the growth curve. The imposed perturbations (confluence, serum starvation, and KCN treatment) are all expected to result in an increase in the ratio of NADH/NAD + . Our studies suggest that the fluorescence lifetime of both the free and the protein-bound components of NADH and the ratio of free to protein-bound NADH is related to changes in the NADH/NAD + ratio.

show abstract

“…39 There is a general agreement on the characteristics of the spectra and their biochemical significance when measured in vivo and in vitro. The absorption and fluorescence spectra of NADH ͑the reduced form͒ have been well characterized at different levels of organizational complexity: in solution, [40][41][42][43] in cell suspension, 44,45 in tissue slices, 46,47 in perfused organs, 48 and under in vivo monitoring. 21,[49][50][51] The conceptual foundations for tissue mitochondrial NADH fluorometry were established 14 in the early 1950s.…”

Section: Mitochondrial Nadh Fluorescencementioning

confidence: 99%

Tissue spectroscope: a novel in vivo approach to real time monitoring of tissue vitality

et al. 2004

View full text Add to dashboard Cite

Optical monitoring of various tissue physiological and biochemical parameters in real-time represents a significant new approach and a tool for better clinical diagnosis. The Tissue Spectroscope (TiSpec), developed and applied in experimental and clinical situations, is the first medical device that enables the real-time monitoring of three parameters representing the vitality of the tissue. Tissue vitality, which is correlated to the oxygen balance in the tissue, is defined as the ratio between O(2) supply and O(2) demand. The TiSpec enables the monitoring of microcirculatory blood flow (O(2) supply), mitochondrial NADH redox state (O(2) balance), and tissue reflectance, which correlates to blood volume. We describe in detail the theoretical basis for the monitoring of the three parameters and the technological aspects of the TiSpec. The comparison between the TiSpec and the existing single parameter monitoring instruments shows a statistically significant correlation as evaluated in vitro as well as in various in vivo animal models. The results presented originated in a pilot study performed in vivo in experimental animals. Further research is needed to apply this technology clinically. The clinical applications of the TiSpec include two situations where the knowledge of tissue vitality can improve clinical practice. The major application is the monitoring of "nonvital" organs of the body [i.e., the skin, gastrointestinal (G-I) tract, urethra] in emergency situations, such as in the operating rooms and intensive care units. Also, the monitoring of specific (vital) organs, such as the brain or the heart, during surgical procedure is of practical importance.

show abstract

NADH spectrofluorometry of rat skin.

Cited by 19 publications

References 0 publications

Lrrc10 is required for early heart development and function in zebrafish

Lrrc10 is required for early heart development and function in zebrafish

Metabolic Mapping of MCF10A Human Breast Cells via Multiphoton Fluorescence Lifetime Imaging of the Coenzyme NADH

Tissue spectroscope: a novel in vivo approach to real time monitoring of tissue vitality

Contact Info

Product

Resources

About