NADP-dependent aromatic alcohol dehydrogenase in polyploid wheats and their diploid relatives. On the origin and phylogeny of polyploid wheats

Jaaska, Vello

doi:10.1007/bf00277370

Cited by 85 publications

(27 citation statements)

References 24 publications

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“…bicornis. This agrees with the widely accepted classification of the section based on isoenzymes (Jaaska 1978), electrophoresis of seedling proteins (Bahrman et al 1988), cpDNA (Bowman et al 1983;Ogihara and Tsunewaki 1988;Wang et al 1997), rRNA (Dvorak 1990), repetitive DNA sequences (Talbert et al 1991), variation in the restriction patterns of repeated nucleotide sequences (Dvorak and Zhang 1992), electrophoresis of leaf proteins (Mendlinger and Zohary 1995), nRFLP (Sasanuma et al 1996;Ciaffi et al 2000;Giorgi et al 2002), in situ hybridisation (Giorgi et al 2003), and CAPS on cpDNA (Haider and Nabulsi 2008).…”

Section: Genetic Relationships Among Triticum Speciessupporting

confidence: 75%

Comparison of the efficiency of A–PAGE and SDS–PAGE, ISSRs and RAPDs in resolving genetic relationships among Triticum and Aegilops species

Haider

Nabulsi

MirAli

2010

Genet Resour Crop Evol

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Species that belong to the genus Triticum L. and the genetically related Aegilops L. genus are important genetic and economic resources because they have an evolutionary relationship with the two main agricultural crops T. aestivum (bread wheat) and T. durum (durum wheat). Therefore, it is important to understand the genetic relationships among the cultivated wheat species and their wild relatives. The latter have a great role in the improvement of cultivated wheat. Molecular markers are the best choice and most reliable means to study these relationships accurately. In this study, we compared the efficiency of the biochemical methods A-PAGE and SDS-PAGE on seed storage proteins and the molecular methods RAPDs and ISSRs to explore the genetic relationships among seven species of Triticum and 20 Aegilops species. Three phylogenetic trees obtained in this study were compared with available classifications and phylogenetic trees constructed earlier for these species. It was noted that the tree based on ISSRs data was the most congruent with those classification and trees. This may be attributed to the fact that ISSRs is more specific, and therefore more reliable. This study is the first to study genetic relationships among all species studied here using biochemical and molecular techniques.

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Section: Genetic Relationships Among Triticum Speciessupporting

confidence: 75%

Comparison of the efficiency of A–PAGE and SDS–PAGE, ISSRs and RAPDs in resolving genetic relationships among Triticum and Aegilops species

Haider

Nabulsi

MirAli

2010

Genet Resour Crop Evol

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“…In our previous study, with the exception of spelt wheat, the Vrn-A1b.2 allele was abundant in T. dicoccoides and T. dicoccum [12], but not in the other tetraploid and hexaploid wheat species analyzed Surprising results were obtained for spelt wheat species (T. spelta and T. macha). Despite the fact that Asian and European spelt are polyphyletic, it is assumed that they were derived from hybridization of hulled emmer T. dicoccum with free-threshing hexaploid wheat T. aestivum [28,29]. For this reason, we expected a split at the VRN-A1 exon 4 and exon 7 haplotypes in some accessions of T. spelta and T. macha, along with accessions carrying one haplotype of this gene.…”

Section: Discussionmentioning

confidence: 92%

Origin and Distribution of the VRN-A1 Exon 4 and Exon 7 Haplotypes in Domesticated Wheat Species

Muterko

Салина

2018

Agronomy

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Abstract:The high adaptive potential of modern wheat to a wide range of environmental conditions is determined by genetic changes during domestication. Genetic diversity in VRN1 genes is a key contributor to this adaptability. Previously, the association between the transitions C->T within the fourth and seventh exons of VRN-A1, the distinguishing pair haplotypes Ex4C/Ex4T and Ex7C/Ex7T, and the modulation of such agronomically valuable traits as the vernalization requirement duration, frost tolerance and flowering time of wheat have been shown. However, this polymorphism was analyzed in only a few cultivars of Triticum aestivum L., and not in other wheat species. In the present study, VRN-A1 exon 4 and exon 7 were investigated in six tetraploid and five hexaploid wheat species carrying different VRN-A1 alleles. An allele-specific polymerase chain reaction (PCR) assay was optimized to identify the VRN-A1 exon 7 haplotypes. It was found that polymorphism of the VRN-A1 exon 7 originated in wild tetraploid wheat of Triticum dicoccoides Körn, while the mutant exon 4 of this gene originated later in domesticated hexaploid wheat of T. aestivum. Both these polymorphisms are found in all hexaploid wheat species. Analysis of the VRN-A1 exon 4 and exon 7 haplotype combinations found that intact exon 7 and mutant exon 4 are associated with analogous types of exon 4 and 7, respectively. With the exclusion of the Vrn-A1c (IL369) and Vrn-A1j alleles, identified only in hexaploid wheat, all dominant VRN-A1 alleles carry intact exons 4 and 7 (Ex4C/7C haplotype). The Ex4C/4T/7T haplotype was detected in numerous accessions of hexaploid wheat and is associated with the presence of multiple copies of VRN-A1. Overall, modern domesticated hexaploid wheat T. aestivum includes most possible combinations of the VRN-A1 exon 4 and exon 7 haplotypes among polyploid wheat, which are present in different proportions. This contributes to the high adaptive potential to a broad range of environmental conditions and facilitates the widespread distribution of this species throughout the world.

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“…This is in line with the few classifications (cited by Bowman et al 1983) that placed Ae. speltoides in a separate subsection Truncata from the other four species (Emarginata), and evidence from isoenzymes (Jaaska 1978), electrophoresis of seedling proteins (Bahrman et al 1988b), cpDNA (Bowman et al 1983;Ogihara and Tsunewaki 1988;Wang et al 1997), rRNA (Dvorak 1990), repetitive DNA sequences (Taberlet et al 1991), variation in the restriction patterns of repeated nucleotide sequences (Dvorak and Zhang 1992), electrophoresis of leaf proteins (Mendlinger and Zohary 1995), nRFLP (Sasanuma et al 1996;Ciaffi et al 2000;Giorgi et al 2002), and in situ hybridisation (Giorgi et al 2003).…”

Section: Discussionmentioning

confidence: 96%

Identification of Aegilops L. species and Triticum aestivum L. based on chloroplast DNA

Haider

Nabulsi

2007

Genet Resour Crop Evol

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The genus Aegilops L. is a very important genetic resource for the breeding of bread wheat Triticum aestivum. Therefore, an accurate and easy identification of Aegilops species is required. Traditionally, identification of Aegilops species has relied heavily on morphological characters. These characters, however, are either not variable enough among Aegilops species or too plastic to be used for identification at the species level. Molecular markers that are more stable within species, therefore, could be the alternative strategy towards an accurate identification. Since the chloroplast DNA has a lower level of evolution compared to the nuclear genome, an attempt was made in this study to investigate polymorphism in the chloroplast DNA among 21 Aegilops species (including Ae. mutica that is now known as Amblyopyrum muticum) and between the latter and T. aestivum to generate markers for the diagnosis of all targeted species. Cleaved amplified polymorphic sequence (CAPS) applied on 22 coding and non-coding chloroplast regions using 80 endonucleases and sequencing of two of those regions revealed little polymorphism between T. aestivum and the various Aegilops species examined and to a less extent was the variation among Aegilops species. Polymorphism observed among species analysed allowed the discrimination of T. aestivum and 12 Aegilops species.

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NADP-dependent aromatic alcohol dehydrogenase in polyploid wheats and their diploid relatives. On the origin and phylogeny of polyploid wheats

Cited by 85 publications

References 24 publications

Comparison of the efficiency of A–PAGE and SDS–PAGE, ISSRs and RAPDs in resolving genetic relationships among Triticum and Aegilops species

Comparison of the efficiency of A–PAGE and SDS–PAGE, ISSRs and RAPDs in resolving genetic relationships among Triticum and Aegilops species

Origin and Distribution of the VRN-A1 Exon 4 and Exon 7 Haplotypes in Domesticated Wheat Species

Identification of Aegilops L. species and Triticum aestivum L. based on chloroplast DNA

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