NADPH oxidase activity in allergenic pollen grains of different plant species

Wang, Xiaoling; Takai, Toshiro; Kamijo, Seiji; Gunawan, Hendra; Ogawa, Hideoki; Okumura, Ko

doi:10.1016/j.bbrc.2009.07.020

Cited by 41 publications

(32 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ragweed pollen-derived NADPH oxidase (8 -10) increases levels of reactive oxygen species in the epithelium and has critical roles in both sensitization to and the development of allergies in mouse models (8,9). Very recently, we have demonstrated that allergenic pollen grains showed NADPH oxidase activity that differed in intensity and localization according to the plant families and that the activity was mostly concentrated within insoluble fractions (11). Pollen grains release substances that have structural similarity with the inflammatory lipid mediators (12,13).…”

mentioning

confidence: 99%

Cupressaceae Pollen Grains Modulate Dendritic Cell Response and Exhibit IgE-Inducing Adjuvant Activity In Vivo

Kamijo¹,

Takai²,

Kuhara³

et al. 2009

The Journal of Immunology

Self Cite

View full text Add to dashboard Cite

Pollen is considered a source of not only allergens but also immunomodulatory substances, which could play crucial roles in sensitization and/or the exacerbation of allergies. We investigated how allergenic pollens from different plant species (Japanese cedar and Japanese cypress, which belong to the Cupressaceae family, and birch, ragweed, and grass) modulate murine bone marrow-derived dendritic cell (DC) responses and examined the effect of Cupressaceae pollen in vivo using mice. DCs were stimulated with pollen extracts or grains in the presence or absence of LPS. Cell maturation and cytokine production in DCs were analyzed by flow cytometry, ELISA, and/or quantitative PCR. Pollen extracts suppressed LPS-induced IL-12 production and the effect was greatest for birch and grass. Without LPS, pollen grains induced DC maturation and cytokine production without IL-12 secretion and the response, for which TLR 4 was dispensable, was greatest for the Cupressaceae family. Intranasal administration of Cupressaceae pollen in mice induced an elevation of serum IgE levels and airway eosinophil infiltration. Coadministration of ovalbumin with Cupressaceae pollen grains induced ovalbumin-specific IgE responses associated with eosinophil infiltration. The results suggest that modulation of DC responses by pollen differs among the plant families via (1) the promotion of DC maturation and cytokine production by direct contact and/or (2) the inhibition of IL-12 production by soluble factors. The strong DC stimulatory activity in vitro and IgE-inducing activity in mice support the clinical relevance of Cupressaceae pollen to allergies in humans.

show abstract

mentioning

confidence: 99%

Cupressaceae Pollen Grains Modulate Dendritic Cell Response and Exhibit IgE-Inducing Adjuvant Activity In Vivo

Kamijo¹,

Takai²,

Kuhara³

et al. 2009

The Journal of Immunology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Still many aspects have to be elucidated, for example putative release of sub-pollen particles from olive pollen upon hydration, superoxide dismutase inhibition of both pollen NOX activities, their presence in insoluble fractions which could facilitate the exposure of tissues to ROS generated by these enzymes etc. These many aspects may contribute to processes like sensitization and pathogenesis of allergic inflammation [7].…”

Section: Discussionmentioning

confidence: 99%

“…Superoxide generated by this enzyme activity locates in particular pollen compartments depending on the plant family: surface, cytoplasm, the outer wall or even in subpollen particles (SPPs) released during hydration [7]. These SPPs owning NADPH oxidase activity can reach the lower airways because their size is in the respirable scale [5] and this activity has been involved in the activation of dendritic cells leading to induction of adaptive immune responses [16].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification and in silico Analysis of NADPH Oxidase Homologues Involved in Allergy from an Olive Pollen Transcriptome

Jiménez-Quesada

Traverso

Zafra

et al. 2015

Bioinformatics and Biomedical Engineering

View full text Add to dashboard Cite

Abstract. Reactive oxygen species generated by pollen NADPH oxidases are present in numerous allergenic pollen species. The superoxide generated by this enzyme has been suggested as a key actor in the induction of allergic inflammation. However, this enzyme has been characterized in Arabidopsis thaliana pollen only, where two pollen-specific genes (RbohH and RbohJ) have been described. The olive (Olea europaea L.) pollen is an important source of allergy in Mediterranean countries. We have assembled and annotated an olive pollen transcriptome, which allowed us to determine the presence of at least two pollen-specific NADPH oxidase homologues. Primers were designed to distinguish between the two homologues, and full-length sequences were obtained through a PCR strategy. Complete in silico analysis of such sequences, including phylogeny, 3-D modeling of the N-terminus, and prediction of cellular localization and post-translational modifications was carried out with the purpose of shed light into the involvement of olive pollen-intrinsic NADPH oxidases in triggering allergy symptoms.

show abstract

“…29 Subsequently, Wang and colleagues described NADPH oxidase activity and localization in several allergenic pollen species. 32 Differences in intensity and localization based on plant families were characterized in the study. Microscopic analyses of grass (Poaceae) and birch (Betulaceae) pollen revealed that pollen NADPH oxidase activity was located in the cytoplasm, plasma membrane, or the outer wall.…”

Section: Air Pollution Influences the Allergic Responsementioning

confidence: 99%

New insights into an old story

Speranza

Scoccianti

2012

Plant Signaling & Behavior

View full text Add to dashboard Cite

ROS is a collective term that includes oxygen radicals (e.g., superoxide, hydroxyle, hydroperoxyle), and certain non radicals (e.g., H 2 O 2 , ozone, singlet oxygen) that are oxidising agents and/or are easily converted into radicals. These agents are continuously produced in plants and other aerobic organisms as a result of partial O 2 reduction during a number of normal metabolic processes, such as respiration, photosynthesis and photorespiration. [1][2][3][4] The ROS pool size depends on the relative rates of ROS generation and destruction, and on the life time of the main ROS species. Plants possess a complex battery of ROS scavenging enzymes (e.g., superoxide dismutase, ascorbate peroxidase, glutathione peroxidase and catalase), and low molecular non-enzymatic antioxidants (e.g., ascorbate and glutathione, but also tocopherol, flavonoids, alkaloids and carotenoids), often confined to particular compartments, that play a key role in ROS cellular control levels. 5,6 Excess ROS is harmful to many plant cell constituents, including lipids, proteins and nucleic acids, and in some case can induce plant cell death. 7,8 Several biochemical and genetic studies have emphasized the key role of ROS as PCD (apoptosis) triggers in plant cell response to specific stimuli.9,10 PCD controlled by ROS occurs during developmental processes, including aleurone cell death and leaf senescence, hypersensitive response, allelopathic plantplant interactions, and various forms of abiotic stress. 6,11,12 External adverse conditions (high light, drought, low and high temperature and mechanical stress) disrupt intracellular ROS Reactive oxygen species (ROS) can exhibit negative and benign traits. In plants, ROS levels increase markedly during periods of environmental stress and defense against pathogen attack. ROS form naturally as a by-product of normal oxygen metabolism, and evenly play an essential role in cell growth. The short ROS lifespan makes them ideal molecules to act in cell signaling, a role they share in both plants and animals. A particular plant organism, the pollen grain, may closely interact with human mucosa and an allergic inflammatory response often results. Pollen grain ROS represent a first, crucial signal which primes and magnifies a cascade of events in the allergic response. New insights into an old storyPollen ROS also play a role in hay fever Keywords: NADPH oxidase, reactive oxygen species, pollen, allergy, oxidative stress, environment pollution homeostasis.6 Interestingly, ROS/redox signaling networks in the chloroplast and mitochondria play essential roles in plant acclimation to abiotic stress via the regulation of important biological pathways, such as gene expression, energy metabolism and protein phosphorylation. 13Until recently, ROS were considered unwelcome by-products of metabolism. However, information continues to emerge on the range of ROS forms, and has demonstrated the molecules serve as important plant development and growth regulators, which operate in strict association with hormonal signal...

show abstract

NADPH oxidase activity in allergenic pollen grains of different plant species

Cited by 41 publications

References 30 publications

Cupressaceae Pollen Grains Modulate Dendritic Cell Response and Exhibit IgE-Inducing Adjuvant Activity In Vivo

Cupressaceae Pollen Grains Modulate Dendritic Cell Response and Exhibit IgE-Inducing Adjuvant Activity In Vivo

Identification and in silico Analysis of NADPH Oxidase Homologues Involved in Allergy from an Olive Pollen Transcriptome

New insights into an old story

Contact Info

Product

Resources

About