NADPH oxidase is involved in regulation of gene expression and ROS overproduction in soybean (Glycine max L.) seedlings exposed to cadmium

Chmielowska-Bąk, Jagna; Arasimowicz‐Jelonek, Magdalena; Izbiańska, Karolina; Frontasyeva, M. V.; Zinicovscaia, Inga; Guiance-Varela, C.; Deckert, Joanna

doi:10.5586/asbp.3551

Cited by 13 publications

(9 citation statements)

References 77 publications

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“…Various biotic and abiotic stresses lead to the production of different ROS such as O 2 − , 1 O 2 , HO • , and H 2 O 2 . Unlike atmospheric oxygen, these molecules are highly reactive, very toxic to the cell, and cause significant damage to various cell structures, membranes, nucleic acids, proteins, enzymes, and lipids [5,24]. As a consequence, plant defense systems are induced which can be classified into enzymatic and non-enzymatic [25].…”

Section: Discussionmentioning

confidence: 99%

“…The activities of the tested antioxidant enzymes were induced in the treated seedling of P. sativum with either Cd or Ni (Figure 3 and Figure 4). A literature review has shown that NADH-oxidase activity was induced due to the induction of various ROS such as O 2 − and H 2 O 2 following biotic and abiotic stresses [24]. The SOD is considered as the first enzyme that triggered under oxidative stress, where it acts directly on superoxide radicals (O 2 − ) which is the precursor of the other ROS [35].…”

Section: Discussionmentioning

confidence: 99%

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Antioxidant System and Biomolecules Alteration in Pisum sativum under Heavy Metal Stress and Possible Alleviation by 5-Aminolevulinic Acid

et al. 2019

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Environmental pollution is the most serious problem that affects crop productivity worldwide. Pisum sativum is a leguminous plant that is cultivated on a large scale in the Nile Delta of Egypt as a winter crop, and many of the cultivated fields irrigated with drainage water that contained many pollutants including heavy metals. The present research aimed to investigate the impact of Cd and Ni on the biochemical and physiological processes in P. sativum and evaluate the potential alleviation of their toxicity by 5-aminolevulinic acid (ALA). Seedlings of P. sativum were grown in Hoagland solution treated with CdCl2 or NiCl2 for 72 h in the growth chamber. Hydrogen peroxide, lipid peroxidation, protein carbonylation, reduced glutathione, oxidized glutathione, proline, phenolics, antioxidant enzymes, as well as Cd and Ni concentrations were measured at 0, 12, 24, 36, 48, 72 h. An experiment of alleviation was conducted where ALA was added to the growth solution at a concentration of 200 µM coupled with 100 µM of either CdCl2 or NiCl2. Hydrogen peroxide, lipid peroxidation, protein carbonylation, reduced glutathione, oxidized glutathione, proline, and phenolics were induced due to the toxicity of Cd and Ni. The activities of antioxidant enzymes [NADH-oxidase (EC: 1.6.3.1), ascorbate peroxidase (EC: 1.11.1.11), glutathione reductase (EC: 1.6.4.2), superoxide dismutase (EC: 1.15.1.1), and catalase (EC: 1.11.1.6)] were induced under the treatments of both metals. On the other hand, the soluble protein decreased gradually depending upon the time of exposure to the heavy metals. The concentration of Cd and Ni in the leaves treated plants increased in time of exposure dependent manner, while their contents remained within the acceptable limits. The addition of ALA decreased the oxidative stress in treated P. sativum plants. The results revealed the significance of using ALA in the cultivation of P. sativum might improve its tolerance against heavy metal stress.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Antioxidant System and Biomolecules Alteration in Pisum sativum under Heavy Metal Stress and Possible Alleviation by 5-Aminolevulinic Acid

et al. 2019

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“…Also our earlier research showed that ROS signal in soybean seedlings exposed to Cd differs in time. The activity of NADPH oxidase modulated expression of signaling associated genes after 3 h of metal treatment, while significant ROS accumulation and lipid peroxidation were noted only after 24 h ( Chmielowska-BąK et al, 2017 ). Apparently, the role of ROS in plant cells is temporal, species, and spatial specific ( Miller et al, 2008 ; Cuypers et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

“…The aim of present study was examination of the influence of Cd in two concentrations (10 and 25 mgl -1 ) on the intensity of oxidative stress and frequency of RNA oxidation-dependent modifications – 8-OHG and AP-sites. Our previous research showed that in the earliest period of Cd stress (3 h), ROS are engaged in the regulation of gene expression, while strong accumulation of O 2 - and H 2 O 2 has been marked after 24 h ( Chmielowska-BąK et al, 2017 ). Therefore, these two time points, 3 and 24 h, were applied in the present study.…”

Section: Introductionmentioning

confidence: 99%

Cadmium Stress Leads to Rapid Increase in RNA Oxidative Modifications in Soybean Seedlings

et al. 2018

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Increase in the level of reactive oxygen species (ROS) is a common response to stress factors, including exposure to metals. ROS over-production is associated with oxidation of lipids, proteins, and nucleic acids. It is suggested that the products of oxidation are not solely the markers of oxidative stress but also signaling elements. For instance, it has been shown in animal models that mRNA oxidation is a selective process engaged in post-transcriptional regulation of genes expression and that it is associated with the development of symptoms of several neurodegenerative disorders. In the present study, we examined the impact of short-term cadmium (Cd) stress on the level of two RNA oxidation markers: 8-hydroxyguanosine (8-OHG) and apurinic/apyrimidinic sites (AP-sites, abasic sites). In the case of 8-OHG, a significant increase was observed after 3 h of exposure to moderate Cd concentration (10 mg/l). In turn, high level of AP-sites, accompanied by strong ROS accumulation and lipid peroxidation, was noted only after 24 h of treatment with higher Cd concentration (25 mg/l). This is the first report showing induction of RNA oxidations in plants response to stress factors. The possible signaling and gene regulatory role of oxidatively modified transcripts is discussed.

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“…This hypothesis was partly supported by Chmielowska et al [ 109 ] when the authors reviewed cadmium sensing in plants. Furthermore, Chmielowska et al [ 110 ] showed that NADPH oxidase activity only occurred after the periods of 6 and 24 h after cadmium stress. In the same study the authors showed that a MAPK cascade gene was activated after 3 h. This could point to MAPK isoform specific activation by superoxide, or that the activation of MAPK can occur independently of superoxide, as well as a time-dependent activation of MAPK by superoxide.…”

Section: The Role Of Other Wall-associated Proteins In Heavy Metalmentioning

confidence: 99%

Decoding Heavy Metal Stress Signalling in Plants: Towards Improved Food Security and Safety

Keyster

Niekerk

Basson

et al. 2020

Plants

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The mining of heavy metals from the environment leads to an increase in soil pollution, leading to the uptake of heavy metals into plant tissue. The build-up of toxic metals in plant cells often leads to cellular damage and senescence. Therefore, it is of utmost importance to produce plants with improved tolerance to heavy metals for food security, as well as to limit heavy metal uptake for improved food safety purposes. To achieve this goal, our understanding of the signaling mechanisms which regulate toxic heavy metal uptake and tolerance in plants requires extensive improvement. In this review, we summarize recent literature and data on heavy metal toxicity (oral reference doses) and the impact of the metals on food safety and food security. Furthermore, we discuss some of the key events (reception, transduction, and response) in the heavy metal signaling cascades in the cell wall, plasma membrane, and cytoplasm. Our future perspectives provide an outlook of the exciting advances that will shape the plant heavy metal signaling field in the near future.

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NADPH oxidase is involved in regulation of gene expression and ROS overproduction in soybean (Glycine max L.) seedlings exposed to cadmium

Cited by 13 publications

References 77 publications

Antioxidant System and Biomolecules Alteration in Pisum sativum under Heavy Metal Stress and Possible Alleviation by 5-Aminolevulinic Acid

Antioxidant System and Biomolecules Alteration in Pisum sativum under Heavy Metal Stress and Possible Alleviation by 5-Aminolevulinic Acid

Cadmium Stress Leads to Rapid Increase in RNA Oxidative Modifications in Soybean Seedlings

Decoding Heavy Metal Stress Signalling in Plants: Towards Improved Food Security and Safety

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