NADPH oxidases, essential players of hormone signalings in plant development and response to stresses

Sun, Li Rong; Zhao, Zhi Jie; Hao, Fu Shun

doi:10.1080/15592324.2019.1657343

Cited by 31 publications

(21 citation statements)

References 48 publications

(64 reference statements)

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“…The available RNA-seq data showed that CsRbohE , clustered in the same group 1 as the AtRbohD gene, was highly expressed in all four tissues ( Figure 5 ). Again, the homologous gene AtrbohD was expressed in all tissues of Arabidopsis and was involved in many physiological processes such as stomatal closure, root formation, and systemic signaling in response to diverse stresses [ 1 , 30 ]. Therefore, we speculate that CsRbohE may have a similar function to AtRbohD .…”

Section: Discussionmentioning

confidence: 99%

Genome Wide Identification of Respiratory Burst Oxidase Homolog (Rboh) Genes in Citrus sinensis and Functional Analysis of CsRbohD in Cold Tolerance

Zhang

Luo

et al. 2022

IJMS

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Respiratory burst oxidase homologs (Rbohs) are critical enzymes involved in the generation of reactive oxygen species (ROS) that play an important role in plant growth and development as well as various biotic and abiotic stresses in plants. Thus far, there have been few reports on the characterization of the Rboh gene family in Citrus. In this study, seven Rboh genes (CsRbohA~CsRbohG) were identified in the Citrus sinensis genome. The CsRboh proteins were predicted to localize to the cell membrane. Most CsRbohs contained four conserved domains, an EF-hand domain, and a transmembrane region. Phylogenetic analysis demonstrated that the CsRbohs were divided into five groups, suggesting potential distinct functions and evolution. The expression profiles revealed that these seven CsRboh genes displayed tissue-specific expression patterns, and five CsRboh genes were responsive to cold stress. Fourteen putative cis-acting elements related to stress response, hormone response, and development regulation were present within the promoters of CsRboh genes. The in-silico microRNA target transcript analyses indicated that CsRbohE might be targeted by csi-miR164. Further functional and physiological analyses showed that the knockdown of CsRbohD in trifoliate orange impaired resistance to cold stress. As a whole, our results provide valuable information for further functional studies of the CsRboh genes in response to cold stress.

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Section: Discussionmentioning

confidence: 99%

Genome Wide Identification of Respiratory Burst Oxidase Homolog (Rboh) Genes in Citrus sinensis and Functional Analysis of CsRbohD in Cold Tolerance

Zhang

Luo

et al. 2022

IJMS

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“…•− -generating NOX during sweet pepper fruit ripening should be also integrated with the NADPH-generating system constituted by a group of NADP-dehydrogenases, which are regulated by signal molecules such as NO and H 2 S (Muñoz-Vargas et al, 2018. Additionally, future analyses should be developed to understand the integration of the redox homeostasis with the phytohormones including ABA, indole-3-acetic acid, gibberellins, cytokinins, or jasmonic acid involved in the ripening of sweet pepper considering that it is a non-climacteric fruit (Symons et al, 2012;Fuentes et al, 2019;Sun et al, 2019). This will allow to extend our knowledge of the complex network of involved signal molecules in this physiological process.…”

Section: Discussionmentioning

confidence: 99%

Superoxide Radical Metabolism in Sweet Pepper (Capsicum annuum L.) Fruits Is Regulated by Ripening and by a NO-Enriched Environment

et al. 2020

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Superoxide radical (O 2•− ) is involved in numerous physiological and stress processes in higher plants. Fruit ripening encompasses degradative and biosynthetic pathways including reactive oxygen and nitrogen species. With the use of sweet pepper (Capsicum annuum L.) fruits at different ripening stages and under a nitric oxide (NO)enriched environment, the metabolism of O 2•− was evaluated at biochemical and molecular levels considering the O 2•− generation by a NADPH oxidase system and its dismutation by superoxide dismutase (SOD). At the biochemical level, seven O 2•−generating NADPH-dependent oxidase isozymes [also called respiratory burst oxidase homologs (RBOHs) I-VII], with different electrophoretic mobility and abundance, were detected considering all ripening stages from green to red fruits and NO environment. Globally, this system was gradually increased from green to red stage with a maximum of approximately 2.4-fold increase in red fruit compared with green fruit. Significantly, breaking-point (BP) fruits with and without NO treatment both showed intermediate values between those observed in green and red peppers, although the value in NOtreated fruits was lower than in BP untreated fruits. The O 2•− -generating NADPH oxidase isozymes I and VI were the most affected. On the other hand, four SOD isozymes were identified by non-denaturing electrophoresis: one Mn-SOD, one Fe-SOD, and two CuZn-SODs. However, none of these SOD isozymes showed any significant change during the ripening from green to red fruits or under NO treatment. In contrast, at the molecular level, both RNA-sequencing and real-time quantitative PCR analyses revealed different patterns with downregulation of four genes RBOH A, C, D, and E during pepper fruit ripening. On the contrary, it was found out the upregulation of a Mn-SOD gene in the ripening transition from immature green to red ripe stages, whereas a Fe-SOD gene was downregulated. In summary, the data reveal a contradictory behavior between activity and gene expression of the enzymes involved in the metabolism of

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“…After imbibition, the resurgence of mitochondrial respiration in the seed may cause electrons to be donated to oxygen as an electron acceptor, leading to ROS production ( Kranner et al, 2010 ). NADPH oxidases, also known as respiratory burst oxidase homologs (Rbohs) after the homology with gp91 phox domain of the mammalian respiratory burst oxidase are main drivers of ROS production in the cell wall space which leads to seed germination through non-enzymatic way and results in radicle elongation and endosperm cap weakening in dicots ( Sun L. R. et al, 2019 ). Rbohs transfers the electron from NADPH or NADH to apoplastic oxygen which results in the production of superoxide radicals and these radicals can directly cleave polysaccharides which will loosens the plant cell walls ( Yang et al, 2020 ).…”

Section: Role Of Reactive Oxygen Species In Seed Germinationmentioning

confidence: 99%

Roles of Reactive Oxygen Species and Mitochondria in Seed Germination

et al. 2021

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Seed germination is crucial for the life cycle of plants and maximum crop production. This critical developmental step is regulated by diverse endogenous [hormones, reactive oxygen species (ROS)] and exogenous (light, temperature) factors. Reactive oxygen species promote the release of seed dormancy by biomolecules oxidation, testa weakening and endosperm decay. Reactive oxygen species modulate metabolic and hormone signaling pathways that induce and maintain seed dormancy and germination. Endosperm provides nutrients and senses environmental signals to regulate the growth of the embryo by secreting timely signals. The growing energy demand of the developing embryo and endosperm is fulfilled by functional mitochondria. Mitochondrial matrix-localized heat shock protein GhHSP24.7 controls seed germination in a temperature-dependent manner. In this review, we summarize comprehensive view of biochemical and molecular mechanisms, which coordinately control seed germination. We also discuss that the accurate and optimized coordination of ROS, mitochondria, heat shock proteins is required to permit testa rupture and subsequent germination.

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NADPH oxidases, essential players of hormone signalings in plant development and response to stresses

Cited by 31 publications

References 48 publications

Genome Wide Identification of Respiratory Burst Oxidase Homolog (Rboh) Genes in Citrus sinensis and Functional Analysis of CsRbohD in Cold Tolerance

Genome Wide Identification of Respiratory Burst Oxidase Homolog (Rboh) Genes in Citrus sinensis and Functional Analysis of CsRbohD in Cold Tolerance

Superoxide Radical Metabolism in Sweet Pepper (Capsicum annuum L.) Fruits Is Regulated by Ripening and by a NO-Enriched Environment

Roles of Reactive Oxygen Species and Mitochondria in Seed Germination

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