NADPH Oxidases: Structure and Function

Quinn, Mark T.

doi:10.1002/9781118355886.ch7

Cited by 3 publications

(11 citation statements)

References 462 publications

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“…Zebrafish nox2 was expressed primarily in both phagocyte types, as expected of a phagocyte NAPDH oxidase (Fig S1A). It had a narrower cellular spectrum of expression than that of its transmembrane partner cyba /p22 phox (Fig S1B), consistent with the known functional partnership of Cyba with other Nox proteins (Quinn, 2013). The cytosolic subunit ncf1/p47 phox of phagocytic NAPDH oxidase displayed a similar pattern of expression.…”

Section: Resultssupporting

confidence: 75%

“…The multimeric phagocyte NADPH oxidase enzymatic complex is also of interest as the prototypic NADPH oxidase, and its NOX2 gp91 phox /CYBB subunit and has provided a paradigm for understanding the structure and function of other NADPH oxidase enzyme complexes (NOX 1, 3-5, and also DUOX 1-2) (Quinn, 2013). Our attempts to validate functional impairment of ROS generation highlight the need to consider both the specificity of the stimulus and the cell types expressing the NADPH oxidase subunit of interest.…”

Section: Discussionmentioning

confidence: 99%

“…ROS production by neutrophils is generated by an active process involving the phagocyte NADPH oxidase (PHOX) enzyme. Neutrophil PHOX is a multi-subunit enzyme with cytosolic (NCF1/p47 phox , NCF2/p67 phox , NCF4/p40 phox ) and transmembrane (CYBA/p22 phox , NOX2/CYBB/gp91 phox ) subunit proteins (Panday et al, 2015; Quinn, 2013; Thomas et al, 2017). CYBC1/EROS chaperones gp91 phox and p22 phox heterodimerization in the endoplasmic reticulum, but is not part of the NADPH oxidase complex itself (Randzavola et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

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Anox2/cybbzebrafish mutant with defective neutrophil ROS production displays normal initial neutrophil recruitment to sterile tail injuries

Isiaku,

Zhang,

Pazhakh

et al. 2024

Preprint

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Reactive oxygen species (ROS) are important effectors and modifiers of the acute inflammatory response, recruiting neutrophils to sites of tissue injury. In turn, neutrophils are both consumers and producers of reactive oxygen species. Stimulated neutrophils generate reactive oxygen species in an oxidative burst through the activity of a multimeric phagocytic NADPH oxidase complex. Mutations in theNOX2/CYBB(previously gp91phox) NADPH oxidase subunit are the commonest cause of chronic granulomatous disease, a disease characterized by infection susceptibility and an inflammatory phenotype. To model chronic granulomatous disease, we made anox2/cybbzebrafish (Danio rerio) mutant and demonstrated it to have severely impaired neutrophil ROS production. Reduced early survival ofnox2mutant embryos indicated an essential requirement fornox2during early development. Innox2/cybbzebrafish mutants, the dynamics of initial neutrophil recruitment to both mild and severe surgical tailfin wounds was normal, suggesting that excessive neutrophil recruitment at the initiation of inflammation is not the primary cause of the sterile inflammatory phenotype of chronic granulomatous disease patients. Thisnox2zebrafish mutant adds to existing in vivo models for studying neutrophil reactive oxygen species function in development and disease.

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Section: Resultssupporting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Anox2/cybbzebrafish mutant with defective neutrophil ROS production displays normal initial neutrophil recruitment to sterile tail injuries

Isiaku,

Zhang,

Pazhakh

et al. 2024

Preprint

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show abstract

“…To be more specific, NADPH oxidase complexes, present in the plasma membrane of both immune and hepatic cells [53], could be stimulated to produce ROS [54] by mechanic contact.…”

Section: Discussionmentioning

confidence: 99%

“…Similarly to Kupffer cells, the hepatic stellate cells express NADPH oxidase 2 (NOX-2), become activated in response to liver injury, and produce ROS [54].…”

Section: Discussionmentioning

confidence: 99%

Complex responses to Si quantum dots accumulation in carp liver tissue: Beyond oxidative stress

Serban

Stanca

Sima

et al. 2015

Chemico-Biological Interactions

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A nox2/cybb zebrafish mutant with defective myeloid cell reactive oxygen species production displays normal initial neutrophil recruitment to sterile tail injuries

Isiaku,

Zhang,

Pazhakh

et al. 2024

G3: Genes, Genomes, Genetics

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Reactive oxygen species are important effectors and modifiers of the acute inflammatory response, recruiting phagocytes including neutrophils to sites of tissue injury. In turn, phagocytes such as neutrophils are both consumers and producers of reactive oxygen species. Phagocytes including neutrophils generate reactive oxygen species in an oxidative burst through the activity of a multimeric phagocytic nicotinamide adenine dinucleotide phosphate oxidase complex. Mutations in the NOX2/CYBB (previously gp91phox) nicotinamide adenine dinucleotide phosphate oxidase subunit are the commonest cause of chronic granulomatous disease, a disease characterized by infection susceptibility and an inflammatory phenotype. To model chronic granulomatous disease, we made a nox2/cybb zebrafish (Danio rerio) mutant and demonstrated it to have severely impaired myeloid cell reactive oxygen species production. Reduced early survival of nox2 mutant embryos indicated an essential requirement for nox2 during early development. In nox2/cybb zebrafish mutants, the dynamics of initial neutrophil recruitment to both mild and severe surgical tailfin wounds was normal, suggesting that excessive neutrophil recruitment at the initiation of inflammation is not the primary cause of the “sterile” inflammatory phenotype of chronic granulomatous disease patients. This nox2 zebrafish mutant adds to existing in vivo models for studying reactive oxygen species function in myeloid cells including neutrophils in development and disease.

show abstract

NADPH Oxidases: Structure and Function

Cited by 3 publications

References 462 publications

Anox2/cybbzebrafish mutant with defective neutrophil ROS production displays normal initial neutrophil recruitment to sterile tail injuries

Anox2/cybbzebrafish mutant with defective neutrophil ROS production displays normal initial neutrophil recruitment to sterile tail injuries

Complex responses to Si quantum dots accumulation in carp liver tissue: Beyond oxidative stress

A nox2/cybb zebrafish mutant with defective myeloid cell reactive oxygen species production displays normal initial neutrophil recruitment to sterile tail injuries

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