NADPH production in dark stages is critical for cyanobacterial photocurrent generation: a study using mutants deficient in oxidative pentose phosphate pathway

Hatano, Jiro; Kusama, Shoko; Tanaka, Kenya; Kohara, Ayaka; Miyake, Chikahiro; Nakanishi, Shuji; Shimakawa, Ginga

doi:10.1007/s11120-022-00903-0

Cited by 17 publications

(4 citation statements)

References 41 publications

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“…23,39 It is known that photosynthesis and respiration in cyanobacteria share redox species for electron transfer. 40–42 When the light is ON, photocurrent immediately began to flow (Fig. 5B).…”

Section: Resultsmentioning

confidence: 99%

Cyanobacteria-based double-mediated photo-microbial electrochemical cells are promising future energy sources for electricity generation and hydrogen production

Lee¹,

Lee²,

Kim³

2023

J. Mater. Chem. A

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Section: Resultsmentioning

confidence: 99%

Cyanobacteria-based double-mediated photo-microbial electrochemical cells are promising future energy sources for electricity generation and hydrogen production

Lee¹,

Lee²,

Kim³

2023

J. Mater. Chem. A

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“…The effects of glycolytic intermediate accumulation on the initiation of photosynthesis are further examined using mutants lacking enzymes involved in dark metabolism. Under dark conditions, cyanobacteria obtain NADPH mainly via the OPP pathway 31–33 . As an initial OPP pathway substrate, glucose 6-phosphate (G6P) is supplied by glycogen degradation through glycogen phosphorylase.…”

Section: Resultsmentioning

confidence: 99%

Dark accumulation of downstream glycolytic intermediates confers robust initiation of photosynthesis in cyanobacteria

Tanaka

Shirai

Matsuda

et al. 2022

Preprint

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In photosynthetic organisms, a smooth metabolic transition from dark to light conditions is essential for efficient CO2 fixation and growth. Dark-to-light transition leads to activation of the enzymes of the Calvin-Benson-Bassham (CBB) cycle and initiates CO2 fixation metabolism using energy and redox equivalents provided by the photosynthetic electron transfer reaction. While many enzymatic mechanisms of the light activation of the CBB cycle have been clarified to date, the metabolic distribution and dynamics underlying the smooth start of photosynthetic metabolism remain unclear. Here, we show rapid (on a time scale of seconds) dynamics of changes in absolute metabolite concentration and 13C tracer incorporation during photosynthetic induction (PI) in the cyanobacterium Synechocystis sp. PCC 6803. By combining these data, we were able to estimate time-resolved nonstationary dynamics of metabolic fluxes during PI. This novel metabolic analysis indicated that the initial step of PI consists of the reduction of 3-phosphoglycerate (3PG), a metabolite that accumulates in dark conditions; notably, this step occurs before subsequent generation of ribulose-1,5-bisphosphate (RuBP) and CO2 fixation by ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). In vivo oxygen evolution measurement in the presence of a CBB cycle inhibitor further confirmed that the accumulated 3PG is a major initial substrate for RuBP generation and functions as a key electron sink before the initiation of CO2 fixation. Our results indicate a fundamental mechanism supporting the seamless activation of photosynthetic metabolism after dark-to-light transitions.

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“…However, characterisation of EET in phototrophs is limited. Cyanobacterial EET uses a diffusible redox mediator accepting electrons downstream of PSI in the PETC 32,[83][84][85] . EET of photosynthetic microorganisms has been widely utilised in R-PETCs 7 .…”

Section: Diversity Of Photosynthetic Electron Transport Chainsmentioning

confidence: 99%

Rewiring photosynthetic electron transport chains for solar energy conversion

Lawrence,

Egan,

Hoefer

et al. 2023

Nat Rev Bioeng

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Photosynthetic organisms have evolved versatile electron transport chains capable of the efficient conversion of solar energy into chemical energy. Researchers can engineer these electron transport pathways to drive new-to-nature processes in a class of systems we term "rewired photosynthetic electron transport chains" (R-PETCs). R-PETCs can be used for the light-driven production of electricity or chemicals and exhibit numerous advantages over the synthetic systems widely used for these applications, including enhanced stability, versatility, and sustainability. In this review we summarise the current state of R-PETC research, highlighting major advances alongside outstanding research problems. We also provide descriptions of R-PETC development, outlining the different classes of R-PETCs, the research tools used in their construction, and the key design considerations important for achieving high-performances. Finally, we identify future avenues for R-PETC research which we expect will enhance performances sufficiently to make these systems suitable for a variety of real-world applications.

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NADPH production in dark stages is critical for cyanobacterial photocurrent generation: a study using mutants deficient in oxidative pentose phosphate pathway

Cited by 17 publications

References 41 publications

Cyanobacteria-based double-mediated photo-microbial electrochemical cells are promising future energy sources for electricity generation and hydrogen production

Cyanobacteria-based double-mediated photo-microbial electrochemical cells are promising future energy sources for electricity generation and hydrogen production

Dark accumulation of downstream glycolytic intermediates confers robust initiation of photosynthesis in cyanobacteria

Rewiring photosynthetic electron transport chains for solar energy conversion

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