NADPH‐related processes studied with a SoxR‐based biosensor in <i>Escherichia coli</i>

Spielmann, Alina; Baumgart, Meike; Bott, Michael

doi:10.1002/mbo3.785

Cited by 7 publications

(2 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the ratio was not significantly changed when pntA was repressed. These results are basically consistent with previous studies (Li et al, 2016;Spielmann et al, 2018). After introducing sRNA plasmids targeting pntA and sthA to strain BS1151 harboring plasmid pAC-6409PS, the pinene titers were measured (Figure 5B).…”

Section: Figure 4 Characterization Of Combinatorial Dynamic Repressionsupporting

confidence: 92%

A dynamic and multilocus metabolic regulation strategy using quorum-sensing-controlled bacterial small RNA

Bao¹,

Jiang²,

Zhu

et al. 2021

Cell Reports

View full text Add to dashboard Cite

Metabolic regulation strategies have been developed to redirect metabolic fluxes to production pathways. However, it is difficult to screen out target genes that, when repressed, improve yield without affecting cell growth. Here, we report a strategy using a quorum-sensing system to control small RNA transcription, allowing cell-density-dependent repression of target genes. This strategy is shown with convenient operation, dynamic repression, and availability for simultaneous regulation of multiple genes. The parameters A i , A m , and R A (3-oxohexanoyl-homoserine lactone [AHL] concentrations at which half of the maximum repression and the maximum repression were reached and value of the maximum repression when AHL was added manually, respectively) are defined and introduced to characterize repression curves, and the variant LuxR I58N is identified as the most suitable tuning factor for shake flask culture. Moreover, it is shown that dynamic overexpression of the Hfq chaperone is the key to combinatorial repression without disruptions on cell growth. To show a broad applicability, the production titers of pinene, pentalenene, and psilocybin are improved by 365.3%, 79.5%, and 302.9%, respectively, by applying combinatorial dynamic repression.

show abstract

Section: Figure 4 Characterization Of Combinatorial Dynamic Repressionsupporting

confidence: 92%

A dynamic and multilocus metabolic regulation strategy using quorum-sensing-controlled bacterial small RNA

Bao¹,

Jiang²,

Zhu

et al. 2021

Cell Reports

View full text Add to dashboard Cite

show abstract

“…7D). Since studies have suggested PntAB to primarily function in the synthesis of NADPH by the oxidation of NADH (Sauer et al ., 2004, Spielmann et al . 2018), it was possible, suppression of growth defect by multi-copy pntAB could follow from an increase in the cellular NADPH pool.…”

Section: Resultsmentioning

confidence: 99%

Evidence for role of transketolase function in the maintenance of pyridine nucleotide levels inEscherichia coli

Vimala

Harinarayanan

2023

Preprint

View full text Add to dashboard Cite

The transketolase (Tkt) activity provides reversible link between glycolysis and pentose phosphate pathway (PPP). Depending on the metabolic flux, it can catalyse synthesis of glycolytic intermediates, fructose-6-phosphate and glyceraldehyde-3-phosphate from xylulose-5-P and ribose-5-P (PPP intermediates) and synthesis of xylulose-5-P from the above mentioned glycolytic intermediates. Using E. coli, we addressed the physiological significance of this metabolic flexibility by studying the growth phenotypes and metabolic changes associated with depletion of transketolase activity and the genetic changes or growth conditions that rescued the growth phenotypes. Tkt function was needed for cell growth when glucose was catabolized solely through Oxidative-PPP. Under gluconeogenic growth conditions, either transketolase or UdhA transhydrogenase was needed for growth. Cells depleted of Tkt activity were more sensitive than wild type to genetic changes that perturb pyridine cofactor levels. In LB medium, Tkt function was needed to prevent growth arrest from the accumulation of ribose-5-P and possibly other pentose phosphates. In cell free extracts, the activity of Zwf and Gnd enzymes that support NADPH synthesis was inhibited by ribose-5-P. These results suggested, Tkt function played an important role in the maintenance of pyridine cofactor pool and this was confirmed by quantification. Metabolomic changes associated with transketolase depletion supported the genetic data.

show abstract

Carbon-negative bio-production of short-chain carboxylic acids (SCCAs) from syngas via the sequential two-stage bioprocess by Moorella thermoacetica and metabolically engineered Escherichia coli

Fei,

Zhang,

Shang

et al. 2025

Bioresource Technology

View full text Add to dashboard Cite

NADPH‐related processes studied with a SoxR‐based biosensor in Escherichia coli

Cited by 7 publications

References 42 publications

A dynamic and multilocus metabolic regulation strategy using quorum-sensing-controlled bacterial small RNA

A dynamic and multilocus metabolic regulation strategy using quorum-sensing-controlled bacterial small RNA

Evidence for role of transketolase function in the maintenance of pyridine nucleotide levels inEscherichia coli

Carbon-negative bio-production of short-chain carboxylic acids (SCCAs) from syngas via the sequential two-stage bioprocess by Moorella thermoacetica and metabolically engineered Escherichia coli

Contact Info

Product

Resources

About