NaF INDUCES EARLY DIFFERENTIATION OF MURINE BONE MARROW CELLS ALONG THE GRANULOCYTIC PATHWAY, BUT NOT THE MONOCYTIC OR PRE-OSTEOCLASTIC PATHWAY, IN VITRO.

Oguro, Akira; Kawase, Tomoyuki; Orikasa, Michiaki

doi:10.1290/0303020.i

Cited by 2 publications

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“…At the end of the treatment, cells were gently harvested with a rubber policeman, and the number of viable cells was counted as assessed by trypan blue dye-exclusion using a hemocytometer [9,16]. In our preliminary study (Orikasa et al, unpublished observations), this mechanical cell-detachment protocol did not significantly reduce cell viability.…”

Section: Evaluation Of Cell Viability and Esterase Expressionmentioning

confidence: 88%

“…Bone marrow cells were isolated from 6-week-old male ddY mice (Japan SLC, Hamamatsu, Japan) immediately after sacrifice by cervical dislocation as described previously [9,16]. This study was performed in accordance with guidelines of the Review Committee for Animal Experimentation of Niigata University, meeting the guidelines for the regulation of animal experimentation (International Committee on Laboratory Animal Science: ICLAS), the CIOMS (Council of International Organizations of Medical Sciences) ethical code for animal experimentation and domestic regulations.…”

Section: Isolation and Treatment Of Murine Bone-marrow Cellsmentioning

confidence: 99%

“…Esterase expression was evaluated in cytochemically stained cells on cover slips with kits for naphthol AS-D chloroacetate esterase (ChAE) (Muto Chemical, Tokyo, Japan) and a-naphthyl bu-tyrate esterase (non-specific esterase; NSE) (Muto Chemical). Cells were examined under a light microscope after counterstaining with Carrazi's hematoxylin [9,16].…”

Section: Evaluation Of Cell Viability and Esterase Expressionmentioning

confidence: 99%

“…In brief, cells treated for 6 days in 48well plates were gently harvested and immediately seeded into 60-well Terasaki plates (Nunc InterMed, Roskilde, Denmark) at a density of 1 10 6 cells/ml (5 l/well). After a 2 h incubation period at 37 8C, 0.025 % polystyrene latex particles in PBS (10 l/ well) (Average diameter, 1.10 m; Sigma, St. Louis, MO) were added to each well, and plates were incubated for a further 2 h. After fixation with 2.5 % glutaraldehyde, the number of cells phagocyting polystyrene beads was visually counted in 200 differentially counted cells as described previously [9,16,17].…”

Section: Assessment Of Phagocytic Activitymentioning

confidence: 99%

“…As described in the previous studies [9,16,17], cells treated for 6 days were lysed in Tris-acetate buffer (pH 7.5) containing 0.15 M KCl and 0.1 % Tritox X-100. After sonication and centrifugation, lactate dehydrogenase (LDH) activity in the crude extracts was determined with the Katayama LDH test (Katayama Chemical Co., Osaka, Japan).…”

Section: Evaluation Of Ldh and Nad(p)h Oxidase Activitiesmentioning

confidence: 99%

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Granulocyte Colony-stimulating Factor Synergistically Augments 1,25-Dihydroxyvitamin D₃-Induced Monocytic Differentiation in Murine Bone Marrow Cell Cultures

Kawase

Oguro²

2004

Horm Metab Res

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In a series of studies, we have reported that 1,25-dihydroxyvitamin D (3), a known stimulator of monocytic differentiation, primes bone marrow progenitor cells or promyelocytic HL-60 cells to the actions of several factors involved in both monocytic and granulocytic differentiation. In the present study, we have further examined the combinational effects of 1,25-dihydroxyvitamin D (3) and the other inducer of granulopoiesis, granulocyte colony-stimulating factor, on non-fractionated native murine bone-marrow cell culture. Over 6 days of treatment, human granulocyte colony-stimulating factor sustained cell viability, increased the size of small rounded non-adherent cells, and induced granulocytic differentiation, while 1,25-dihydroxyvitamin D (3) decreased cell viability, promoted the development of large adherent flattened cells, and upregulated some monocytic differentiation markers. Combining these two factors over 6 days synergistically upregulated phagocyte activity, membrane-bound interleukin-1alpha, NAD(P)H oxidase, monocytic Mac-1, and non-specific esterase. Similar effects were observed in successive treatment with granulocyte colony-stimulating factor followed by 1,25-dihydroxyvitamin D (3), but successive treatment in reverse order was somewhat less effective. No combinational treatment upregulated granulocytic lactate dehydrogenase, Gr-1, or chloroacetate esterase to as great an extent as was obtained with granulocyte colony-stimulating factor alone, indicating that granulocytic differentiation is attenuated by addition of 1,25-dihydroxyvitamin D (3). Therefore, in contrast to our previous data, the present findings suggest that granulocyte colony-stimulating factor synergistically augments 1,25-dihydroxyvitamin D (3)-induced monocytic differentiation in our murine bone-marrow cell cultures. Considering previously published data, we also suggest that these synergistic effects may be mainly due to the combination of two distinct effects such as the primary proliferative effects of granulocyte colony-stimulating factor on multipotent stem cells and the subsequent differentiative effects of 1,25-dihydroxyvitamin D (3) on proliferating cells.

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Section: Evaluation Of Cell Viability and Esterase Expressionmentioning

confidence: 88%

Section: Isolation and Treatment Of Murine Bone-marrow Cellsmentioning

confidence: 99%

Section: Evaluation Of Cell Viability and Esterase Expressionmentioning

confidence: 99%

Section: Assessment Of Phagocytic Activitymentioning

confidence: 99%

Section: Evaluation Of Ldh and Nad(p)h Oxidase Activitiesmentioning

confidence: 99%

See 3 more Smart Citations

Granulocyte Colony-stimulating Factor Synergistically Augments 1,25-Dihydroxyvitamin D₃-Induced Monocytic Differentiation in Murine Bone Marrow Cell Cultures

Kawase

Oguro²

2004

Horm Metab Res

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Fluoride’s Effects on the Formation of Teeth and Bones, and the Influence of Genetics

2010

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Fluorides are present in the environment. Excessive systemic exposure to fluorides can lead to disturbances of bone homeostasis (skeletal fluorosis) and enamel development (dental/enamel fluorosis). The severity of dental fluorosis is also dependent upon fluoride dose and the timing and duration of fluoride exposure. Fluoride's actions on bone cells predominate as anabolic effects both in vitro and in vivo. More recently, fluoride has been shown to induce osteoclastogenesis in mice. Fluorides appear to mediate their actions through the MAPK signaling pathway and can lead to changes in gene expression, cell stress, and cell death. Different strains of inbred mice demonstrate differential physiological responses to ingested fluoride. Genetic studies in mice are capable of identifying and characterizing fluoride-responsive genetic variations. Ultimately, this can lead to the identification of at-risk human populations who are susceptible to the unwanted or potentially adverse effects of fluoride action and to the elucidation of fundamental mechanisms by which fluoride affects biomineralization.

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NaF INDUCES EARLY DIFFERENTIATION OF MURINE BONE MARROW CELLS ALONG THE GRANULOCYTIC PATHWAY, BUT NOT THE MONOCYTIC OR PRE-OSTEOCLASTIC PATHWAY, IN VITRO.

Cited by 2 publications

References 0 publications

Granulocyte Colony-stimulating Factor Synergistically Augments 1,25-Dihydroxyvitamin D₃-Induced Monocytic Differentiation in Murine Bone Marrow Cell Cultures

Granulocyte Colony-stimulating Factor Synergistically Augments 1,25-Dihydroxyvitamin D₃-Induced Monocytic Differentiation in Murine Bone Marrow Cell Cultures

Fluoride’s Effects on the Formation of Teeth and Bones, and the Influence of Genetics

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NaF INDUCES EARLY DIFFERENTIATION OF MURINE BONE MARROW CELLS ALONG THE GRANULOCYTIC PATHWAY, BUT NOT THE MONOCYTIC OR PRE-OSTEOCLASTIC PATHWAY, IN VITRO.

Cited by 2 publications

References 0 publications

Granulocyte Colony-stimulating Factor Synergistically Augments 1,25-Dihydroxyvitamin D3-Induced Monocytic Differentiation in Murine Bone Marrow Cell Cultures

Granulocyte Colony-stimulating Factor Synergistically Augments 1,25-Dihydroxyvitamin D3-Induced Monocytic Differentiation in Murine Bone Marrow Cell Cultures

Fluoride’s Effects on the Formation of Teeth and Bones, and the Influence of Genetics

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Product

Resources

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Granulocyte Colony-stimulating Factor Synergistically Augments 1,25-Dihydroxyvitamin D₃-Induced Monocytic Differentiation in Murine Bone Marrow Cell Cultures

Granulocyte Colony-stimulating Factor Synergistically Augments 1,25-Dihydroxyvitamin D₃-Induced Monocytic Differentiation in Murine Bone Marrow Cell Cultures