Nagina 22 mutants tolerant or sensitive to low P in field show contrasting response to double P in hydroponics and pots

Yugandhar, Poli; Nallamothu, Veronica; Panigrahy, Madhusmita; Tipireddy, Sitaramamma; Bhadana, Vijai Pal; Voleti, S. R.; Subrahmanyam, D.; Mangrauthia, Satendra K.; Neelamraju, Sarla

doi:10.1080/03650340.2018.1471684

Cited by 9 publications

(9 citation statements)

References 17 publications

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“…Increase in APase activity was observed in roots, which increased with the decreasing Pi content in the hydroponic medium. Increase in APase activity was reported earlier by Yugandhar et al [ 67 ] in rice under P-deficiency stress. Up-regulated expression of the genes coding for enzymes/proteins responsible for organic acid synthesis and exudation under P-deficiency stress has been reported earlier [ 68 – 70 ].…”

Section: Discussionsupporting

confidence: 78%

Characterization of contrasting rice (Oryza sativa L.) genotypes reveals the Pi-efficient schema for phosphate starvation tolerance

et al. 2021

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Background Phosphorus (P), being one of the essential components of nucleic acids, cell membranes and enzymes, indispensable for diverse cellular processes like photosynthesis/carbohydrate metabolism, energy production, redox homeostasis and signaling. Crop yield is severely affected due to Phosphate (Pi) deficiency; and to cope with Pi-deficiency, plants have evolved several strategies. Some rice genotypes are compatible with low Pi availability, whereas others are sensitive to Pi deficiency. However, the underlying molecular mechanism for low Pi tolerance remains largely unexplored. Result Several studies were carried out to understand Pi-deficiency responses in rice at seedling stage, but few of them targeted molecular aspects/responses of Pi-starvation at the advanced stage of growth. To delineate the molecular mechanisms for low Pi tolerance, a pair of contrasting rice (Oryza sativa L.) genotypes [viz. Pusa-44 (Pi-deficiency sensitive) and its near isogenic line (NIL-23, Pi-deficiency tolerant) harboring Phosphorus uptake 1 (Pup1) QTL from an aus landrace Kasalath] were used. Comparative morphological, physiological, and biochemical analyses confirmed some of the well-known findings. Transcriptome analysis of shoot and root tissues from 45-day-old rice plants grown hydroponically under P-sufficient (16 ppm Pi) or P-starved (0 ppm Pi) medium revealed that Pi-starvation stress causes global transcriptional reprogramming affecting several transcription factors, signaling pathways and other regulatory genes. We could identify several significantly up-regulated genes in roots of NIL-23 under Pi-starvation which might be responsible for the Pi starvation tolerance. Pathway enrichment analysis indicated significant role of certain phosphatases, transporters, transcription factors, carbohydrate metabolism, hormone-signaling, and epigenetic processes in improving P-starvation stress tolerance in NIL-23. Conclusion We report the important candidate mechanisms for Pi acquisition/solubilization, recycling, remobilization/transport, sensing/signalling, genetic/epigenetic regulation, and cell wall structural changes to be responsible for P-starvation tolerance in NIL-23. The study provides some of the novel information useful for improving phosphorus-use efficiency in rice cultivars.

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Section: Discussionsupporting

confidence: 78%

Characterization of contrasting rice (Oryza sativa L.) genotypes reveals the Pi-efficient schema for phosphate starvation tolerance

et al. 2021

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“…5A). On the contrary, the activity of Apase increased significantly during Pi deficiency in the root (N22 [2.17 fold], NH787 [2.86 fold]) and in the shoot (N22 [4.31 fold], NH787 [4.46 fold]) (data not shown) and was coherent with earlierstudies on N22 and its EMS mutants46,47 . Although the augmenting effect of Pi deficiency on Apase activity was significantly higher in the root of NH787compared with N22, it was comparable in the shoot of these two genotypes.…”

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confidence: 90%

“…of India, for generating EMS-mutagenized M2 populations (~85,000) in the background of N22 41 . At the ICAR-IIRR, efforts are underway for more than a decade to screen N22 EMS mutants that exhibited altered PUE under field condition, which led to the identification of several loss-of-function and gain-of-function mutants [42][43][44][45][46][47] . Among these mutants, detailed morphophysiological and molecular analyses were carried out for the lossof-function mutant NH101, which revealed several traits that were affected contributing towards its lower PUE compared with wild-type N22 47 .…”

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confidence: 99%

Gain-of-Function NH787 Ethylmethanesulfonate Mutant of Nagina22 Rice Variety Confers Augmented PUE

Yugandhar

Nallamothu

et al. 2021

Preprint

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Rice (Oryza sativa L.), a major dietary source, is often cultivated in soils poor in available inorganic orthophosphate (Pi), which is a pivotal nutrient for growth and development. Poor soils are amended by phosphorus (P) fertilizer, which is derived from the non-renewable rock phosphate reserves. Therefore, there is a need for developing rice varieties with high productivity under low P conditions. At the ICAR-IIRR, ethyl methanesulfonate (EMS) mutagenized rice genotype Nagina22 (N22) were screened for high grain yield in Pi-deprived soil, which led to the identification of ~10 gain-of-function mutants including NH787. Here, detailed comparative morphophysiological, biochemical, and molecular analyses of N22 and NH787 were carried out in hydroponics and potting soil under different Pi regimes. Under Pi-deprived conditions, compared with N22, NH787 exhibited higher root and vegetative biomass, the number of tillers, and grain yield. The augmented agronomic traits of NH787 were corroborated with significantly higher photosynthetic rate, pollen fertility, stigma receptivity, and the activities of antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT). Further, several genes involved in the maintenance of Pi homeostasis (GPH) were differentially regulated. The study thus revealed a wide-spectrum influence of the mutation in NH787 that contributed towards its higher Pi use efficiency.

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“…Among these nutrients, phosphorus (P), secondary to nitrogen (N), plays an essential role in plant growth. Phosphorus fixation is a common problem in the soils of rice fields ( Krishnamurthy et al, 2010 ; Panigrahy et al, 2014 ; Vandamme et al, 2016 ; Yugandhar et al, 2018a , b ). The applied P is not completely available to plants because of slow diffusion rates and processes such as fixation in alkaline soils with Ca and fixation in acidic soils with Fe and Al.…”

Section: Introductionmentioning

confidence: 99%

Increased Catalase Activity and Maintenance of Photosystem II Distinguishes High-Yield Mutants From Low-Yield Mutants of Rice var. Nagina22 Under Low-Phosphorus Stress

et al. 2018

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An upland rice variety, Nagina22 (N22) and its 137 ethyl methanesulfonate (EMS)-induced mutants, along with a sensitive variety, Jaya, was screened both in low phosphorus (P) field (Olsen P 1.8) and in normal field (Olsen P 24) during dry season. Based on the grain yield (YLD) of plants in normal field and plants in low P field, 27 gain of function (high-YLD represented as hy) and 9 loss of function (low-YLD represented as ly) mutants were selected and compared with N22 for physiological and genotyping studies. In low P field, hy mutants showed higher P concentration in roots, leaves, grains, and in the whole plant than in ly mutants at harvest. In low P conditions, Fv/Fm and qN were 24% higher in hy mutants than in ly mutants. In comparison with ly mutants, the superoxide dismutase (SOD) activity in the roots and leaves of hy mutants in low P fields was 9% and 41% higher at the vegetative stage, respectively, but 51% and 14% lower in the roots and leaves at the reproductive stage, respectively. However, in comparison with ly mutants, the catalase (CAT) activity in the roots and leaves of hy mutants in low P fields was 35% higher at the vegetative stage and 15% and 17% higher at the reproductive stage, respectively. Similarly, hy mutants in low P field showed 20% and 80% higher peroxidase (POD) activity in the roots and leaves at the vegetative stage, respectively, but showed 14% and 16% lower POD activity at the reproductive stage in the roots and leaves, respectively. Marker trait association analysis using 48 simple sequence repeat (SSR) markers and 10 Pup1 gene markers showed that RM3648 and RM451 in chromosome 4 were significantly associated with grain YLD, tiller number (TN), SOD, and POD activities in both the roots and leaves in low P conditions only. Similarly, RM3334 and RM6300 in chromosome 5 were associated with CAT activity in leaves in low P conditions. Notably, grain YLD was positively and significantly correlated with CAT activity in the roots and shoots, Fv/Fm and qN in low P conditions, and the shoots’ P concentration and qN in normal conditions. Furthermore, CAT activity in shoots was positively and significantly correlated with TN in both low P and normal conditions. Thus, chromosomal regions and physiological traits that have a role in imparting tolerance to low P in the field were identified.

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Nagina 22 mutants tolerant or sensitive to low P in field show contrasting response to double P in hydroponics and pots

Cited by 9 publications

References 17 publications

Characterization of contrasting rice (Oryza sativa L.) genotypes reveals the Pi-efficient schema for phosphate starvation tolerance

Characterization of contrasting rice (Oryza sativa L.) genotypes reveals the Pi-efficient schema for phosphate starvation tolerance

Gain-of-Function NH787 Ethylmethanesulfonate Mutant of Nagina22 Rice Variety Confers Augmented PUE

Increased Catalase Activity and Maintenance of Photosystem II Distinguishes High-Yield Mutants From Low-Yield Mutants of Rice var. Nagina22 Under Low-Phosphorus Stress

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