Naloxone and alum synergistically augment adjuvant activities of each other in a mouse vaccine model of Salmonella typhimurium infection

Jazani, Nima Hosseini; Parsania, Somayeh; Sohrabpour, Mohammad; Mazloomi, Ebrahim; Karimzad, M; Shahabi, Shahram

doi:10.1016/j.imbio.2010.10.005

Cited by 23 publications

(24 citation statements)

References 42 publications

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“…Aluminum salts are often used with CpG ODNs to enhance and/or balance the immunogenicity of many different types of antigens [264,434,435]. Naloxone (an opioid receptor antagonist) has been recently evaluated with aluminum salts with use [436,437]. Liposomes can easily accommodate another adjuvant for enhanced immunogenicity, such as CpG ODNs [119,[438][439][440], TLR7 ligand-3M-019 [441], Quil-A [442], MPL [443], and DNA [444].…”

Section: Adjuvant Combinationsmentioning

confidence: 99%

Selection of Adjuvants for Enhanced Vaccine Potency

Wang¹,

Singh²

2011

WJV

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Section: Adjuvant Combinationsmentioning

confidence: 99%

Selection of Adjuvants for Enhanced Vaccine Potency

Wang¹,

Singh²

2011

WJV

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“…regulatory T cells. Previous investigations have shown that vaccination based on an opioid antagonist as an adjuvant such as naloxone or naltrexone (which are structurally similar pure opiate receptor antagonists but it has a longer biologic half-life) and propranolol (as BetaAdrenergic Receptor Antagonist) could improve protection against various intracellular infections in BALB/c mice via modulation in the Th1/Th2 balance or affecting macrophages through binding to Toll-like receptors (Jazani et al 2010(Jazani et al , 2011Molla Hassan et al 2009;Mohammadzadeh Hajipirloo et al 2014;Shahabi et al 2014). Furthermore, it has been shown that in mice and humans, naloxone increases T lymphocytes proliferation, increases the NK cells activity, and accelerates local inflammation via a direct effect on monocytes, macrophages and dendritic cells.…”

Section: Discussionmentioning

confidence: 99%

“…There are experiments showing that naloxone can enhance lymphocyte proliferation and shift the immune response from Th2 to Th1 in murine model (Sacerdote et al 1998(Sacerdote et al , 2000. Furthermore various studies have shown that naloxone is capable of inducing protective immune responses when used as an adjuvant in combination with different pathogens, such as Salmonella typhimurium, Listeria monocytogenes, and Herpes simplex virus type 1 (HSV-1), indicating the ability of naloxone as an adjuvant (Jamali et al 2007;Jazani et al 2010;Jazani et al 2011). On the basis of these findings, we evaluated the immunogenicity potential of heat-killed Leishmania major promastigotes in combination with the mixture of alum and naloxone as an adjuvant against Leishmania major infection in BALB/c as a susceptible mouse model.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the alum–naloxone adjuvant activity against experimental murine leishmaniasis due to L. major

et al. 2016

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Leishmaniasis is caused by intracellular parasites of Leishmania species, which are transmitted by the bite of the sandfly. Recovery and protection against the infection depends on the induction of a strong Th1 type of immune response. Vaccination of mice with the opioid antagonist naloxone can promote the activation of the Th1 responses. We studied the efficacy of the mixture of naloxone and alum, as an adjuvant, to enhance immune responses and induce protection against Leishmania major infection in BALB/c as a susceptible mouse model. BALB/ c mice were immunized with Ag-naloxone-alum, Agalum, Ag-naloxone or PBS subcutaneously three times at 2-week intervals. The humoral and cellular specific immune responses were assessed 2 weeks after the last immunization and compared with the control mice. Our results indicated that the administration of alum-naloxone as an adjuvant increased the capability of L. major promastigote antigens to enhance lymphocyte proliferation, the levels of IFN-c, and the IFN-c/IL-5 ratio. The results of DTH showed that there were no significant differences in footpad swelling between the groups of immunized mice as compared with the non-vaccinated control group; however, no significant differences were observed in the survival rate among groups. It can be concluded that although immunization with the alum-naloxone mixture in combination with the autoclaved L. major promastigote antigens could enhance cellular immunity and shift the immune response to a Th1 pattern, it could not protect the mice against Leishmania major infection.

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“…Naloxone is an antagonist of morphine-like receptors that is commonly used to reduce the side effects of drug abuse such as respiratory depression (20,21). Several studies have shown that naloxone, as an opioid receptors antagonist, has adjuvant effect.…”

Section: Introductionmentioning

confidence: 99%

Immune Responses of Mice Immunized with HBsAg Formulated in Naloxone/Alum Mixture: Comparison to Fendrix Vaccine

et al. 2017

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Background: Hepatitis B virus (HBV) can cause cirrhosis of the liver and hepatocellular carcinoma. Due to the lack of sufficient immune response in whole population, several studies have been conducted to improve the efficacy of alum-based HBV vaccine. Here, naloxone/alum mixture as adjuvant was used for the hepatitis B surface antigen HBsAg vaccine and immune parameters evaluated in immunized mice. Objectives: The present study aimed at investigating the effect of naloxone/alum mixture for the HBsAg vaccine and comparing to Fendrix vaccine. Methods: Female Balb/c mice were vaccinated at day 0, 14, and 28 with alum-based vaccine or naloxone/alum mixture vaccine in different doses. Naloxone/alum vaccine groups received a dose of 3, 6, or 10 mg/kg of naloxone (NLX) in the vaccine formulation. One group received routine HBsAg alum vaccine and another group received Fendrix vaccine. Some groups received naloxone plus HBsAg without alum and a group received HBsAg without adjuvant. Phosphate buffered saline (PBS), naloxone, and alum were also injected into the control groups separately. Finally, the naloxone/alum formulated vaccine was compared with the Fendrix and routine alum-based vaccine with respect to the levels of total anti-HBS antibody, IFN-γ, IL-4, IgG1, IgG2a, and the level of lymphocyte proliferation. Results:The level of total anti-HBS antibody in naloxone formulated vaccine was comparable with Fendrix. Meanwhile, IFN-γ/IL-4 ratio level was significantly higher in naloxone formulated vaccine groups versus mere vaccine group. IgG2a was also higher in the naloxone formulated vaccine groups. Conclusions: Based on the data presented in the present study, it was found that naloxone/alum mixture has the ability to shift the immune response towards Th1 pattern and potentially increase immunity against infections.

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Naloxone and alum synergistically augment adjuvant activities of each other in a mouse vaccine model of Salmonella typhimurium infection

Cited by 23 publications

References 42 publications

Selection of Adjuvants for Enhanced Vaccine Potency

Selection of Adjuvants for Enhanced Vaccine Potency

Evaluation of the alum–naloxone adjuvant activity against experimental murine leishmaniasis due to L. major

Immune Responses of Mice Immunized with HBsAg Formulated in Naloxone/Alum Mixture: Comparison to Fendrix Vaccine

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