Nannochloropsis oculata feed additive alleviates mercuric chloride-induced toxicity in Nile tilapia (Oreochromis niloticus)

Mamdouh, Alzahraa; Zahran, Eman; Zahran, Faten; Zaki, Viola

doi:10.1016/j.aquatox.2021.105936

Cited by 7 publications

(5 citation statements)

References 68 publications

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“…The absence of inflammation in the intestine and gills reflects the anti-inflammatory and antioxidant effect of N. oculata . These results are agreed with our previous studies that showed feeding N. oculata reduces the histopathology of Hg 2+ on the liver, kidney, and gills and has an anti-inflammatory effect on Nile tilapia intestine and liver [ 26 , 81 ].…”

Section: Discussionsupporting

confidence: 93%

“…Besides, tilapia fish was selected as a model because it shows high resistance against water pollution during environmental toxicological studies [ 24 , 25 ]. In line with our previous findings [ 26 ], the effects of mercury exposure have been studied extensively. Still, researchers have mostly focused on the cellular level, paying less attention to how exposure affects growth performances or gene expressions.…”

Section: Introductionsupporting

confidence: 76%

“…Ready-to-use N. oculata dry powder was purchased from the National Research Center, Cairo, Egypt. N. oculata -supplemented diets were prepared, as described in our previous study [ 26 ]. Two medicated diets were formulated by supplementing Nile tilapia basal feed with N. oculata powder at two different doses: 50 g/kg dry feed (i.e., 5% N. oculata ) or 100 g/kg dry feed (i.e., 10% N. oculata ).…”

Section: Methodsmentioning

confidence: 99%

“…The fish were exposed to a sub-lethal concentration of 0.3 mg/L HgC1 2 for up to 21 days. This concentration equates to 1/4th of the median lethal concentration of 1.21 mg/L based on a 96-h toxicological assay in Nile tilapia [ 26 ]. To summarize, a new daily stock solution of 1000 mg/L mercury was made by dissolving the calculated amount of HgCl 2 in one liter of double-distilled water.…”

Section: Methodsmentioning

confidence: 99%

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Toxicity Evaluation, Oxidative, and Immune Responses of Mercury on Nile Tilapia: Modulatory Role of Dietary Nannochloropsis oculata

Zahran¹,

Ahmed²,

Hassan³

et al. 2023

Biol Trace Elem Res

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The current study evaluated the potential ameliorative effect of a dietary immune modulator, Nannochloropsis oculata microalga, on the mercuric chloride (HgCl2)-induced toxicity of Nile tilapia. Nile tilapia (45–50 g) were fed a control diet or exposed to ¼ LC50 of HgCl2 (0.3 mg/L) and fed on a medicated feed supplemented with N. oculata (5% and 10% (50 or 100 g/kg dry feed)) for 21 days. Growth and somatic indices, Hg2+ bioaccumulation in muscles, and serum acetylcholinesterase (AChE) activity were investigated. Antioxidant and stress-related gene expression analyses were carried out in gills and intestines. Histopathological examinations of gills and intestines were performed to monitor the traits associated with Hg2+ toxicity or refer to detoxification. Hg2+ toxicity led to significant musculature bioaccumulation, inhibited AChE activity, downregulated genes related to antioxidants and stress, and elicited histopathological changes in the gills and intestine. Supplementation with N. oculata at 10% was able to upregulate the anti-oxidative-related genes while downregulated the stress apoptotic genes in gills and intestines compared to the unexposed group. In addition, minor to no histopathological traits were detected in the gills and intestines of the N. oculata-supplemented diets. Our data showed the benefit of dietary N. oculata in suppressing Hg2+ toxicity, which might support its efficacy as therapeutic/preventive agent to overcome environmental heavy metal pollution in aquatic habitats.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionsupporting

confidence: 76%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Toxicity Evaluation, Oxidative, and Immune Responses of Mercury on Nile Tilapia: Modulatory Role of Dietary Nannochloropsis oculata

Zahran¹,

Ahmed²,

Hassan³

et al. 2023

Biol Trace Elem Res

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“…Fish ( n = 15 fish/tank, 45 fish/group) were allocated into three groups in triplicate: control (basal diet), N5 (basal diet supplemented with 5% of N. oculata ), and N10 (basal diet supplemented with 10% of N. oculata ). Inclusion levels were selected based on our previous investigation (Mamdouh et al 2021). Fish were hand‐fed twice per day at 0900 and 1600 hours at 3% of their biomass (on a dry matter basis).…”

Section: Methodsmentioning

confidence: 99%

Nannochloropsis oculata supplementation improves growth, immune response, intestinal integrity, and disease resistance of Nile Tilapia

Salem

Adawy²,

Zaki

et al. 2022

J Aqua Anim Hlth

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Objective:The current study evaluated the potential roles of incorporating Nannochloropsis oculata into the diet of Nile Tilapia Oreochromis niloticus in an 8week trial. Methods: Dietary supplementation of N. oculata was tested at inclusion levels (0%[control], 5% [N5], and 10% [N1]) in triplicate. After the trial, comprehensive fish health indicators were evaluated.Result: N. oculata-supplemented feed had a stimulatory effect on fish body weight, where a significant increase in final weight and specific growth rate was observed in the N10 group compared to the control. Better feed conversion was observed at N5 and N10 compared to control. Organosomatic indices were elevated significantly in the N5 group compared to the N10 and control groups. Serum lysozyme activity was significantly increased in the N10 group compared to N5 and control groups. Levels of IgM were significantly higher in N10 compared to the control and N5 groups, with no significance between the latter. Amylase activity showed a significant enhancement in N10 compared to N5. Both levels of N. oculata preserved hepatic health and antioxidant status. Light and transmission electron microscopy showed that Nile Tilapia fed N. oculata at both levels enhanced intestinal immunity, integrity, and absorptive efficiency. The protecting effect of N. oculata was confirmed against Aeromonas hydrophila challenge, where cumulative mortalities were significantly decreased in N5 and N10 groups compared with the control and more in N10. Conclusion:This work confirmed the different beneficial roles of N. oculata dietary supplementation for a Nile Tilapia balanced diet.

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Ameliorative effects of Sargassum kjellmanianum on hexavalent chromium-induced growth inhibition, immune suppression, and oxidative stress in yellow catfish

Shi,

Hu,

Wen

et al. 2024

J Appl Phycol

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Nannochloropsis oculata feed additive alleviates mercuric chloride-induced toxicity in Nile tilapia (Oreochromis niloticus)

Cited by 7 publications

References 68 publications

Toxicity Evaluation, Oxidative, and Immune Responses of Mercury on Nile Tilapia: Modulatory Role of Dietary Nannochloropsis oculata

Toxicity Evaluation, Oxidative, and Immune Responses of Mercury on Nile Tilapia: Modulatory Role of Dietary Nannochloropsis oculata

Nannochloropsis oculata supplementation improves growth, immune response, intestinal integrity, and disease resistance of Nile Tilapia

Ameliorative effects of Sargassum kjellmanianum on hexavalent chromium-induced growth inhibition, immune suppression, and oxidative stress in yellow catfish

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