Nano- and Micro-Patterning of Gold Nanoparticles on PEG- Based Hydrogels for Controlling Cell Adhesion

Yesildag, Cigdem; Zhang, Zhenfang; Ren, Fang; Vicente, G.; Lensen, Marga C.

doi:10.5772/intechopen.71548

Cited by 3 publications

(8 citation statements)

References 63 publications

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“…Hydrogels are three-dimensional networks of hydrophilic polymers, which can imbibe high amounts of water relative to their own weight and are widely used in industry as care products, agriculture, biology, and medicine (Drury and Mooney, 2003; Peppas and Hoffman, 2013). Recent research fields are focused on applying specific functionality to the hydrogel (Haraguchi and Takehisa, 2002; Schexnailder and Schmidt, 2009; Gaharwar et al, 2015; Motealleh and Kehr, 2017; Ren et al, 2017a; Yesildag et al, 2018a). Notably multi-functional hydrogels are highly desired as biomaterials that simultaneously exhibit various beneficial properties (Tkachenko et al, 2003; Sukhorukov et al, 2007; Chen et al, 2013; Li et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Not only molecules but also inorganic nanoparticles provide assorted amounts of functions. Especially gold nanoparticles (Au NPs), which have unique size- and shape -dependent optical properties via surface plasmons, little toxicity, easy synthesis procedures, are desired materials not only for industry, and catalysis but also for biology and medicine (Ren et al, 2017a,b; Yesildag et al, 2017a, 2018a,b).…”

Section: Introductionmentioning

confidence: 99%

“…As shown in our previous publications, amino-silanized surfaces can be effectively covered with citrate capped Au NPs with high density (Ren et al, 2017a,b; Yesildag et al, 2018b). Hereby the Au NPs were interacting electrostatically with the amino-silane surface and they could be effectively transferred onto PEG hydrogel surfaces by various methods, which we recently published (Ren et al, 2017a,b; Yesildag et al, 2017a,b, 2018a,b).…”

Section: Introductionmentioning

confidence: 99%

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Micro-Patterning of PEG-Based Hydrogels With Gold Nanoparticles Using a Reactive Micro-Contact-Printing Approach

et al. 2019

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In this work a novel, relatively simple, and fast method for patterning of gold nanoparticles (Au NPs) on poly(ethylene glycol) (PEG)-based hydrogels is presented. In the hereby exploited reactive micro-contact printing (reactive-μ-CP) process, the surface of a micro-relief patterned PDMS-stamp is first functionalized with an amino-silane self-assembled monolayer (SAM), which is then inked with Au NPs. The stamp is subsequently brought into conformal contact with thiol-functionalized PEG-based hydrogel films. Due to the strong gold-thiol interactions the Au NPs are adequately and easily transferred onto the surfaces of these soft, multifunctional PEG hydrogels. In this way, defined μ-patterns of Au NPs on PEG hydrogels are achieved. These Au NPs patterns allow specific biomolecular interactions on PEG surfaces, and cell adhesion has been studied. Cells were found to effectively adhere only on Au NPs micro-patterns and to avoid the anti-adhesive PEG background. Besides the cell adhesion studies, these Au NPs μ-patterns can be potentially applied as biosensors in plasmon-based spectroscopic devices or in medicine, e.g., for drug delivery systems or photothermal therapies.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Micro-Patterning of PEG-Based Hydrogels With Gold Nanoparticles Using a Reactive Micro-Contact-Printing Approach

et al. 2019

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“…57 In the second step, the Au NP pattern is transferred onto the PEG hydrogel surface by one of our recently developed transfer methodologies, in particular the “dry” transfer using thiolated PEG gels and the wet deprinting approach. 39 − 43 …”

Section: Introductionmentioning

confidence: 99%

“…This enables the introduction of specific binding sites onto the surface of PEG hydrogels at designed locations and in an optimal density for studying specific biointeraction and manipulating − In the past years, we have exploited several strategies to enable adhesion, spreading, and migration of fibroblast cells on intrinsically nonadhesive PEG-hydrogels. We have fabricated topographic, elastic, and chemical patterns on the hydrogels’ surface, both at the micro- and nano-scale and achieved great control over the cellular responses. − …”

Section: Introductionmentioning

confidence: 99%

Micropatterning of Au NPs on PEG Hydrogels Using Different Silanes To Control Cell Adhesion on the Nanocomposites

2018

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Amino-silanization of silica-based substrates has proven to be effective in guiding the immobilization of citrate-stabilized Au NPs in a good, homogeneous fashion. This accomplishment has formed the basis of fabricating micropatterns of Au NPs on such substrates by patterning of oxidized silicon wafers with (3-aminopropyl)trimethoxysilane (amino-silane) using the microcontact printing (μCP) process. This micropattern of amino-silane is used to specifically adsorb Au NPs. To avoid unspecific adsorption to the nonsilanized areas on the silicon wafers, the nonstamped areas were backfilled with self-assembled monolayers of organosilanes, for example, with methyl- or perfluoro-end-groups. Finally, after having fabricated a micropattern of Au NPs on silicon wafers, the Au NP patterns were transferred onto poly(ethylene glycol) hydrogels by our newly developed procedures, and on these nanocomposite materials, controlled cell adhesion has been achieved. Furthermore, these materials are great candidates for plasmon-based biosensor applications and also for various medical applications, such as for drug delivery systems or photothermal therapies.

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Preparation and application of poly (hydroxyl ethyl methacrylate) nanocomposite hydrogels containing iron oxide nanoparticles as wound dressing

Paydayesh

Heleil

Dadkhah

2022

Polymers and Polymer Composites

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In recent years, polymeric hydrogels are widespread in the field of biological materials such as wound dressing and wound care. In this work, we report for the first time the preparation and application of pHEMA nanocomposite hydrogels containing iron oxide nanoparticles as wound dressings. For this purpose, nanocomposite hydrogels based on poly (hydroxyl ethyl methacrylate) (pHEMA) and various amounts of 5, 10, and 15 wt% iron oxide nanoparticles were successfully prepared via radical polymerization. The structure and morphology of nanocomposite hydrogels were determined by Fourier transform infrared spectroscopy (FTIR) and field emission scanning electron microscope (FE-SEM), respectively. The results of gel fraction and the degree of swelling of hydrogels demonstrated that the gel percentage of pHEMA increased, and the degree of swelling decreased with increasing the percentage of nanoparticles. The WVRT and the porosity of hydrogels decreased by increasing the quantity of nanoparticles and were suitable for wound dressing applications. The effect of iron oxide nanoparticles on the mechanical properties of nanocomposite hydrogels was also studied using compression test and hardness shore A durometer. The results indicated that the compression strength, modulus, strain, and hardness are steadily increasing compared to pure hydrogel by adding nanoparticles. The maximum increase was obtained for a hydrogel sample with 15 wt% iron oxide nanoparticles. Antibacterial properties and biocompatibility were determined by the disk-diffusion and MTT assay methods, respectively. Based on the results, nanocomposite hydrogels exhibited higher percentages of cell survival and better antibacterial properties compared to pure pHEMA.

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Nano- and Micro-Patterning of Gold Nanoparticles on PEG- Based Hydrogels for Controlling Cell Adhesion

Cited by 3 publications

References 63 publications

Micro-Patterning of PEG-Based Hydrogels With Gold Nanoparticles Using a Reactive Micro-Contact-Printing Approach

Micro-Patterning of PEG-Based Hydrogels With Gold Nanoparticles Using a Reactive Micro-Contact-Printing Approach

Micropatterning of Au NPs on PEG Hydrogels Using Different Silanes To Control Cell Adhesion on the Nanocomposites

Preparation and application of poly (hydroxyl ethyl methacrylate) nanocomposite hydrogels containing iron oxide nanoparticles as wound dressing

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