Nano-Antimicrobial Solutions Using Synthetic-Natural Hybrid Designs

Mocan, Teodora; Moşteanu, Ofelia; Matea, Cristian; Pop, Teodora; Al-Hajjar, Nadim; Puia, Cosmin; Furcea, Luminiţa; Bura, Cristina; Mocan, Lucian

doi:10.5772/intechopen.81156

Cited by 4 publications

(3 citation statements)

References 54 publications

(53 reference statements)

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“…Therefore, some of the iron may be released from the decomposed citrate during longterm incubation. It was also important to determine the effect of O-SWCNTs on the growth of S. oneidensis MR-1 because, unlike many other contaminants, CNTs are known to possess antimicrobial activity (Kang et al, 2008;Liu et al, 2009;Mocan et al, 2017). At concentrations below 30 μg/mL, O-SWCNTs enhanced the growth of S. oneidensis MR-1, whereas at concentrations above 40 μg/mL, they inhibited growth.…”

Section: Transformation Of O-swcnts During Degradation By S Oneidensi...mentioning

confidence: 99%

Long-term continuous degradation of carbon nanotubes by a bacteria-driven Fenton reaction

Takahashi,

Hori

2023

Front. Microbiol.

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Very few bacteria are known that can degrade carbon nanotubes (CNTs), and the only known degradation mechanism is a Fenton reaction driven by Labrys sp. WJW with siderophores, which only occurs under iron-deficient conditions. No useful information is available on the degradation rates or long-term stability and continuity of the degradation reaction although several months or more are needed for CNT degradation. In this study, we investigated long-term continuous degradation of oxidized (carboxylated) single-walled CNTs (O-SWCNTs) using bacteria of the genus Shewanella. These bacteria are widely present in the environment and can drive the Fenton reaction by alternating anaerobic-aerobic growth conditions under more general environmental conditions. We first examined the effect of O-SWCNTs on the growth of S. oneidensis MR-1, and it was revealed that O-SWCNTs promote growth up to 30 μg/mL but inhibit growth at 40 μg/mL and above. Then, S. oneidensis MR-1 was subjected to incubation cycles consisting of 21-h anaerobic and 3-h aerobic periods in the presence of 30 μg/mL O-SWCNTs and 10 mM Fe(III) citrate. We determined key factors that help prolong the bacteria-driven Fenton reaction and finally achieved long-term continuous degradation of O-SWCNTs over 90 d. By maintaining a near neutral pH and replenishing Fe(III) citrate at 60 d, a degraded fraction of 56.3% was reached. S. oneidensis MR-1 produces Fe(II) from Fe(III) citrate, a final electron acceptor for anaerobic respiration during the anaerobic period. Then, ·OH is generated through the Fenton reaction by Fe(II) and H2O2 produced by MR-1 during the aerobic period. ·OH was responsible for O-SWCNT degradation, which was inhibited by scavengers of H2O2 and ·OH. Raman spectroscopy and X-ray photoelectron spectroscopy showed that the graphitic structure in O-SWCNTs was oxidized, and electron microscopy showed that long CNT fibers initially aggregated and became short and isolated during degradation. Since Shewanella spp. and iron are ubiquitous in the environment, this study suggests that a Fenton reaction driven by this genus is applicable to the degradation of CNTs under a wide range of conditions and will help researchers develop novel methods for waste treatment and environmental bioremediation against CNTs.

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Section: Transformation Of O-swcnts During Degradation By S Oneidensi...mentioning

confidence: 99%

Long-term continuous degradation of carbon nanotubes by a bacteria-driven Fenton reaction

Takahashi,

Hori

2023

Front. Microbiol.

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“…Biocompatibility, non-toxicity, ease of characterization, and high stability are some of the significant properties of AuNPs that promote their diverse applications (Cabuzu et al, 2015;Hu et al, 2020). So far, there are successful reports for the use of these compounds to detect various viruses and bacterial agents (Mocan et al, 2017;Singh et al, 2017). Antibody-based sensors or immunosensors, are a popular format of biosensors that change the transducer signal when a specific antigen-antibody reaction is detected (Cho et al, 2020).…”

Section: Immunosensorsmentioning

confidence: 99%

Rapid-format recombinant antibody-based methods for the diagnosis of Clostridioides difficile infection: Recent advances and perspectives

et al. 2022

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Clostridioides difficile, the most common cause of nosocomial diarrhea, has been continuously reported as a worldwide problem in healthcare settings. Additionally, the emergence of hypervirulent strains of C. difficile has always been a critical concern and led to continuous efforts to develop more accurate diagnostic methods for detection of this recalcitrant pathogen. Currently, the diagnosis of C. difficile infection (CDI) is based on clinical manifestations and laboratory tests for detecting the bacterium and/or its toxins, which exhibit varied sensitivity and specificity. In this regard, development of rapid diagnostic techniques based on antibodies has demonstrated promising results in both research and clinical environments. Recently, application of recombinant antibody (rAb) technologies like phage display has provided a faster and more cost-effective approach for antibody production. The application of rAbs for developing ultrasensitive diagnostic tools ranging from immunoassays to immunosensors, has allowed the researchers to introduce new platforms with high sensitivity and specificity. Additionally, DNA encoding antibodies are directly accessible in these approaches, which enables the application of antibody engineering to increase their sensitivity and specificity. Here, we review the latest studies about the antibody-based ultrasensitive diagnostic platforms for detection of C. difficile bacteria, with an emphasis on rAb technologies.

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“…Metal nanoparticles and especially gold nanoparticles (GNPs) have attracted a lot of interest from the scientific community due to their unique properties such as: large surface-to-volume ration, optoelectronic properties, rich functionality, low toxicity and high biocompatibility [1,2]. Recent advances in the fields of nanoparticles synthesis and functionalization resulted in novel medical applications [3,4]. One area of biomedical research in which GNPs have center stage is the development of carriers for cellular delivery of drugs, proteins and non-cell-penetrating peptides [5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%