Nano-bioengineered sensing technologies for real-time monitoring of reactive oxygen species in in vitro and in vivo models

Kumari, Rohini; Dkhar, Daphika S.; Mahapatra, Supratim; Divya, .; Kumar, Rahul; Chandra, Pranjal

doi:10.1016/j.microc.2022.107615

Cited by 11 publications

(7 citation statements)

References 138 publications

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“…The •OH radical is formed in the presence of H 2 O 2 in a two-stage process: the Haber–Weiss and Fenton reactions. The radical destroys membranes by interacting with lipids and proteins [ 32 ]. The extract concentration of 1.0 mg/mL showed the best results, increasing the chronological lifespan and survival of yeast under oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Ex Vivo and In Vitro Antiaging and Antioxidant Extract Activity of the Amelanchier ovalis from Siberia

Asyakina

Atuchin

Drozdova

et al. 2022

IJMS

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Phenolic acids are biologically active substances that prevent aging and age-related diseases, e.g., cancer, cardiovascular diseases, Alzheimer’s disease, Parkinson’s disease, etc. Cellular senescence is related to oxidative stress. The Siberian Federal District is rich in medicinal plants whose extracts contain phenolic acids. These plants can serve as raw materials for antiaging, antioxidant food supplements, and Amelanchier ovalis is one of them. In the present research, we tested the phytochemical profile of its extract for phenolic acids. Its geroprotective and antioxidant properties were studied both ex vivo and in vitro using Saccharomyces cerevisiae Y-564 as a model organism. The chromotographic analysis revealed gallic, p-hydroxybenzoic, and protocatechuic acids, as well as derivatives of chlorogenic and gallic acids. The research involved 0.25, 0.5, and 1.0 mg/mL extracts of Amelanchier ovalis, all of which increased the growth and lifespan of yeast cells. In addition, the extracts increased the survival rate of yeast under oxidative stress. An in vitro experiment also demonstrated the antioxidant potential of Amelanchier ovalis against ABTS radicals. Therefore, the Amelanchier ovalis berry extract proved to be an excellent source of phenolic acids and may be recommended as a raw material for use in antioxidant and geroprotective food supplements.

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Section: Discussionmentioning

confidence: 99%

Ex Vivo and In Vitro Antiaging and Antioxidant Extract Activity of the Amelanchier ovalis from Siberia

Asyakina

Atuchin

Drozdova

et al. 2022

IJMS

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“…Therefore, rigorous safety and efficacy evaluations of these biomaterial platforms are necessary before considering their administration in patients [ 293 ]. Furthermore, it is essential to develop more practical diagnostic methods that allow real-time monitoring of ROS levels in pathological areas of the human body [ 297 ]. Such advancements would enable the precise determination of optimal doses and administration routes for NZ@hydrogels that ultimately maximize their clinical benefits.…”

Section: Outlook and Challengesmentioning

confidence: 99%

Nanozyme-Engineered Hydrogels for Anti-Inflammation and Skin Regeneration

Kurian,

Singh,

Sagar

et al. 2024

Nano-Micro Lett.

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Inflammatory skin disorders can cause chronic scarring and functional impairments, posing a significant burden on patients and the healthcare system. Conventional therapies, such as corticosteroids and nonsteroidal anti-inflammatory drugs, are limited in efficacy and associated with adverse effects. Recently, nanozyme (NZ)-based hydrogels have shown great promise in addressing these challenges. NZ-based hydrogels possess unique therapeutic abilities by combining the therapeutic benefits of redox nanomaterials with enzymatic activity and the water-retaining capacity of hydrogels. The multifaceted therapeutic effects of these hydrogels include scavenging reactive oxygen species and other inflammatory mediators modulating immune responses toward a pro-regenerative environment and enhancing regenerative potential by triggering cell migration and differentiation. This review highlights the current state of the art in NZ-engineered hydrogels (NZ@hydrogels) for anti-inflammatory and skin regeneration applications. It also discusses the underlying chemo-mechano-biological mechanisms behind their effectiveness. Additionally, the challenges and future directions in this ground, particularly their clinical translation, are addressed. The insights provided in this review can aid in the design and engineering of novel NZ-based hydrogels, offering new possibilities for targeted and personalized skin-care therapies.

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“…H 2 O 2 is a byproduct of multiple enzymatic pathways that regulate an array of cellular operations, including cell motility, differentiation, and proliferation. At lower intracellular concentrations (10 –8 M), it governs proliferation of cells; at 10 –6 M, it induces cell growth arrest; and at 10 –4 M, it causes cell death. , However, at higher doses, H 2 O 2 causes irreversible sulfinic and sulfonic connection formation, irrevocably harming proteins and other components in the cell. This gives rise to a variety of medical problems, such as aging, cardiovascular disease, inflammation, neurodegenerative disorders, cancer, and so on. , Furthermore, H 2 O 2 serves as a mediator molecule for the indirect sensing of glucose, excessive levels of which cause various disorders, including diabetes, a global health problem. − There are multiple analytical approaches for detecting H 2 O 2 , including spectrometry, fluorimetry, titrimetry, chromatography, and electrochemical techniques. , However, electrochemical approaches have been widely used due to their promptness, cost effectiveness, selectivity, simplicity, and high accuracy.…”

Section: Introductionmentioning

confidence: 99%

Smartphone-Integrated Automated Sensor Employing Electrochemically Engineered 3D Bimetallic Nanoflowers for Hydrogen Peroxide Quantification in Milk

Kumari,

Mendki,

Chandra

2024

Langmuir

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Hydrogen peroxide (H2O2), one of the reactive oxygen species in living beings, serves as a regulator of various cellular processes. However, excessive peroxide concentrations are linked to oxidative stress and promptly disrupt cellular components, leading to several pathological conditions in the body. Moreover, it is extremely reactive and has a limited lifetime; thus, H2O2 sensing remains a prominent focus of research. Enzymatic sensing probes were widely employed to detect H2O2 in the recent past; however, they are susceptible to intrinsic chemical and thermal instabilities, which decrease the reliability and durability of the surface. This research was designed to come up with a feasible solution to this problem. Herein, a novel nonenzymatic peroxidase-mimic three-dimensional (3D) bimetallic nanoflower has been synergistically engineered for quick sensing of H2O2. The sensor platform showed minimal resistance or enhanced charge transfer properties as well as remarkable analytical capability, having a broad linear range between 0.01 and 1 nM and a detection limit of 1.46 ± 0.07 pM. The probe responded to changes in H2O2 concentration in just 2.10 ± 0.02 s, making it a quick sensing platform for H2O2 tracking. This peroxidase-mimic nanozyme probe showed minimal sensitivity to interferants often seen in real-world sample matrices and possessed good recoveries ranging from 92.88 to 99.09% in milk samples. Further, a facile and user-friendly smartphone application (APP) named “H Peroxide‑Check ” was developed and integrated into the sensor to check the milk adulteration by detecting H2O2. It processes the current output obtained from the sensing interface and provides real-time peroxide concentrations in milk. The entire procedure of fabricating the probe is a single, highly robust step that takes only 10 min and is coupled with a smartphone APP, highlighting the sensor’s quick manufacturing and deployment for automated H2O2 monitoring in industrial and point-of-care settings.

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Nano-bioengineered sensing technologies for real-time monitoring of reactive oxygen species in in vitro and in vivo models

Cited by 11 publications

References 138 publications

Ex Vivo and In Vitro Antiaging and Antioxidant Extract Activity of the Amelanchier ovalis from Siberia

Ex Vivo and In Vitro Antiaging and Antioxidant Extract Activity of the Amelanchier ovalis from Siberia

Nanozyme-Engineered Hydrogels for Anti-Inflammation and Skin Regeneration

Smartphone-Integrated Automated Sensor Employing Electrochemically Engineered 3D Bimetallic Nanoflowers for Hydrogen Peroxide Quantification in Milk

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