Nano-CeO2 decorated graphene based chitosan nanocomposites as enzymatic biosensing platform: fabrication and cellular biocompatibility assessment

Abstract

The present study summarizes the designing of a green transducer phase based on nano-cerium oxide (CeO2) decorated reduced graphene oxide (RGO) reinforced chitosan nanocomposites as an effective enzyme immobilizer and bio-sensing matrix for glucose analyte. Also, it scrutinizes the biocompatibility and cell viability of the synthesized nanohybrid with human fibroblastic macrophage cell line. CeO2 nanoparticles (NPs) were successfully grown on graphene nanosheet in the presence of cationic surfactant followed by facile hydrothermal treatment. The eventual growth of synthesized CeO2 nanocrystals on the graphene layer was confirmed from X-ray diffraction (XRD), transmission electron microscopy (TEM) and Raman analysis. The biocompatibility of the synthesized nanohybrid was also evident from the MTT assay. Glucose oxidase (GOx) was employed on the green polymer nanocomposites modified FTO electrode to fabricate an enzymatic bioelectrode. The electroanalytical response of the GOx/nano-CeO2/RGO/CS/FTO bioelectrode towards electrooxidation of glucose analyte was investigated by electrochemical impedance (EIS) and cyclic voltammetry (CV) study. The resulting biosensor exhibited a good electrochemical response to glucose within the linear detection range of 0.05-6.5 mM with a low detection limit of 2 μM and a sensitivity of 7.198 μA mM(-1) cm(-2). The bioelectrode also showed good shelf life (~10 weeks) and negligible interfering ability under controlled environment. The obtained results indicate that nano-CeO2/RGO nanohybrid based chitosan nanocomposites achieve a biocompatible biosensing platform for effective enzyme immobilization due to the excellent synergistic effects between the CeO2 nanoparticles and graphene sheet.

Three-Dimensional CeO2 Woodpile Nanostructures To Enhance Performance of Enzymatic Glucose Biosensors

“…Table compares the sensitivity, limit of detection, value of the apparent Michaelis–Menten constant ( K m app ), and linear range with other CeO 2 -based glucose sensors reported from literature. ,,,− These works mainly focus on CeO 2 with nanoscale morphologies (e.g., nanorod, nanoporous) or composite materials with CeO 2 . Although we use commercially available particles, these relatively simple and scalable woodpile structures achieve comparable-to-better sensor performance among enzymatic glucose sensors with CeO 2 -based nanomaterials.…”

CeO2 Nanoparticle-Containing Polymers for Biomedical Applications: A Review

“…The same electrochemical principle was realised in a biosensor fabricated by immobilizing horseradish peroxidase on polyaniline-cerium oxide nano-composite film onto indium-tin-oxide (ITO) coated glass substrate [128]. Chitosan nanocomposites reinforced with nanoceria-decorated reduced graphene oxide may also be used as an effective glucose oxidase immobilizer and bio-sensing matrix for glucose determination [129].…”

Advanced nanomaterials for electrochemical sensors: application in wearable tear glucose sensing technology

“…There are two main strategies: (1) synthesizing electrocatalysts to oxidatively reduce H 2 O 2 to reduce the operating potential to the optimal interference-free range. Most of these electrocatalysts are noble metal nanomaterials such as Au 54,59–61 and Pt, 62–64 and transition metal compounds such as PB, 5,65–68 Ti 3 C 2 , 69–73 CeO 2 , 74,75 and MnO 2 . 42,43,76 Among many nanomaterials, PB exhibits excellent electrocatalytic reduction of H 2 O 2 at low potentials (generally between −0.2 and 0.075 V).…”