Nano-Chitosan/Eucalyptus Oil/Cellulose Acetate Nanofibers: Manufacturing, Antibacterial and Wound Healing Activities

Abstract

Accelerated wound healing in infected skin is still one of the areas where current therapeutic tactics fall short, which highlights the critical necessity for the exploration of new therapeutic approaches. The present study aimed to encapsulate Eucalyptus oil in a nano-drug carrier to enhance its antimicrobial activity. Furthermore, in vitro, and in vivo wound healing studies of the novel nano-chitosan/Eucalyptus oil/cellulose acetate electrospun nanofibers were investigated. Eucalyptus oil showed a potent antimicrobial activity against the tested pathogens and the highest inhibition zone diameter, MIC, and MBC (15.3 mm, 16.0 μg/mL, and 256 μg/mL, respectively) were recorded against Staphylococcus aureus. Data indicated a three-fold increase in the antimicrobial activity of Eucalyptus oil encapsulated chitosan nanoparticle (43 mm inhibition zone diameter against S. aureus). The biosynthesized nanoparticles had a 48.26 nm particle size, 19.0 mV zeta potential, and 0.45 PDI. Electrospinning of nano-chitosan/Eucalyptus oil/cellulose acetate nanofibers was conducted, and the physico-chemical and biological properties revealed that the synthesized nanofibers were homogenous, with a thin diameter (98.0 nm) and a significantly high antimicrobial activity. The in vitro cytotoxic effect in a human normal melanocyte cell line (HFB4) proved an 80% cell viability using 1.5 mg/mL of nano-chitosan/Eucalyptus oil/cellulose acetate nanofibers. In vitro and in vivo wound healing studies revealed that nano-chitosan/Eucalyptus oil/cellulose acetate nanofibers were safe and efficiently enhanced the wound-healing process through enhancing TGF-β, type I and type III collagen production. As a conclusion, the manufactured nano-chitosan/Eucalyptus oil/cellulose acetate nanofiber showed effective potentiality for its use as a wound healing dressing.

Anti-inflammation study of cellulose-chitosan biocomposite-based Tetrapleura tetraptera (Taub) dried fruits aqueous extract

“…Only the highest peak is used in the size calculation leading to similar grain size for chitosan and the biocomposite [33]. The cellulose-chitosan biocomposite size of 53 nm is inside the range of other chitosan hybrid materials (46-58 nm) obtained using Eucalyptus oils [34]. Maxima of PXRD peaks were found to be varied with increasing content of chitosan in blended lms or by material grinding using a planetary ball mill [25,35].…”

Precise healing of oral and maxillofacial wounds: tissue engineering strategies and their associated mechanisms

“…Chitosan nanofibers, eucalyptus oil, and cellulose acetate nanofibers have also been used to prepare wound dressings ( Elbhnsawi et al, 2023 ). In this context, the arrangement of fibers is crucial in attracting bacterial cells and ensuring their adhesion to the nanofiber surface; on the other hand, the inclusion of 1,8-eucalyptin in eucalyptus oil enhances the permeability of bacterial cytoplasmic membranes ( Aldoghaim et al, 2018 ).…”

Bacteria-Derived Cellulose Membranes Modified with Graphene Oxide-Silver Nanoparticles for Accelerating Wound Healing

“…Different substances are incorporated into cellulose and derivatives to contribute to the antimicrobial action of dressings, such as natural oils, − metal ions, − or a mixture with antimicrobial polymers. − Nanomaterials, particularly graphene oxide (GO) and its composites, have intrinsic properties that make them suitable for biomedical applications. These materials exhibit high specific surface area, biocompatibility, and hydrophilicity.…”