Nano-composite of silk fibroin–chitosan/Nano ZrO2 for tissue engineering applications: Fabrication and morphology

Teimouri, Abbas; Ebrahimi, Raheleh; Emadi, Rahmatollah; Beni, Batool Hashemi; Chermahini, Alireza Najafi

doi:10.1016/j.ijbiomac.2015.02.023

Cited by 73 publications

(36 citation statements)

References 52 publications

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“…Topographic SEM images and μ‐CT analysis showed that all scaffolds included interconnected pores with no differences with the increased nHA addition. We expect that the increase in the amount of nHA produce a denser structure for the material, altering the pore density or modifying the interconnection between them, as previously reported (Kim et al, ; Lima et al, ; Saravanan, Leena, & Selvamurugan, ; Teimouri, Ebrahimi, Emadi, Beni, & Chermahini, ). However, the nanometric size of the nHA seems not to affect the structure of the material enough to reduce the mean pore diameter compared to the scaffold with 0% nHA.…”

Section: Discussionsupporting

confidence: 61%

Nanobiocomposite based on natural polyelectrolytes for bone regeneration

Belluzo

Medina

Molinuevo

et al. 2020

J Biomedical Materials Res

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Section: Discussionsupporting

confidence: 61%

Nanobiocomposite based on natural polyelectrolytes for bone regeneration

Belluzo

Medina

Molinuevo

et al. 2020

J Biomedical Materials Res

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“…A distinct advantage results from the high biocompatibility and processability into a wide range of shapes, e.g. fibres, sponges, films, hydrogels and composites [8][9][10][11][12][13][14][15][16]. Fibroin dissolves in a number of concentrated aqueous salt solutions, such as CaCl 2 /water/ethanol (CWE) [12,15,[17][18][19][20], CaNO 3 /methanol/water [21] and LiBr [14,20,[22][23][24][25][26], NaSCN [27].…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Fibroin Solution State in Calcium Chloride/Water/Ethanol for Improved Understanding of the Regeneration Process

Ngo

Bechtold

2018

Fibres and Textiles in Eastern Europe

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Shaping of fibroin protein from Bombyx mori from calcium chloride/water/ethanol solution is of high interest for the manufacturing of biocompatible structures. Potentiometric titration experiments of the dissolved state permit new insight into the solution state of fibroin as a basis for improved regeneration. Titration experiments and infrared spectroscopy of the solution state support the model of an ion-rich hydration layer and interaction of the solvent with charged and polar groups of the fibroin, rather than through formation of defined calcium complexes. The potentiometric titration curves indicate the formation of calcium complexes at pH values above 9, most probably through involvement of basic amino acids and phenolic groups of tyrosine. After regeneration by the addition of methanol, the regenerated protein was characterised by FTIR, nitrogen and calcium analysis. The analysis of supernatants for their residual protein content indicated minor losses of protein, most probably low molecular weight proteins. The results contribute to an improved understanding of the solution state as a basis for larger scale regeneration, e.g. for the coating of textile fibres with regenerated fibroin.

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“…Several studies evaluated bone reaction against optimized zirconia scaffold surfaces [9]. Studies ranging from cell culture to full scale animal models have indicated that osteointegration observed on optimized zirconia surfaces was equal if not superior to different materials, namely titanium alloy.…”

Section: Introductionmentioning

confidence: 99%

Osteogenesis ability of CAD/CAM porous zirconia scaffolds enriched with nano-hydroxyapatite particles

Aboushelib

Shawky

2017

Int J Implant Dent

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BackgroundThe aim of this study was to evaluate osteogenesis ability of CAD/CAM porous zirconia scaffolds enriched with hydroxy apatite used to augment large boney defects in a dog model.MethodsSurgical defects were made bilaterally on the lower jaw of 12 Beagle dogs. Cone beam CT images were used to create three dimensional images of the healed defects. Porous zirconia scaffolds were fabricated by milling custom made CAD/CAM blocks into the desired shape. After sintering, the pores of half of the scaffolds were filled with a nano-hydroxy apatite (HA) powder while the other half served as control. The scaffolds were inserted bilaterally in the healed mandibular jaw defects and were secured in position by resorbable fixation screws. After a healing time of 6 weeks, bone-scaffold interface was subjected to histomorphometric analysis to detect the amount of new bone formation. Stained histological sections were analyzed using a computer software (n=12, α=0.05). Mercury porosimetery was used to measure pore sizes, chemical composition was analyzed using energy dispersive x-ray analysis (EDX), and the crystal structure was identified using x-ray diffraction micro-analysis (XRD).ResultsHA enriched zirconia scaffolds revealed significantly higher volume of new bone formation (33% ± 14) compared to the controls (21% ± 11). New bone deposition started by coating the pore cavity walls and proceeded by filling the entire pore volume. Bone in-growth started from the surface of the scaffold and propagated towards the scaffold core. Islands of entrapped hydroxy apatite particles were observed in mineralized bone matrix.ConclusionsWithin the limitations of this study, hydroxy apatite enhanced osteogenesis ability of porous zirconia scaffolds.

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Nano-composite of silk fibroin–chitosan/Nano ZrO2 for tissue engineering applications: Fabrication and morphology

Cited by 73 publications

References 52 publications

Nanobiocomposite based on natural polyelectrolytes for bone regeneration

Nanobiocomposite based on natural polyelectrolytes for bone regeneration

Analysis of the Fibroin Solution State in Calcium Chloride/Water/Ethanol for Improved Understanding of the Regeneration Process

Osteogenesis ability of CAD/CAM porous zirconia scaffolds enriched with nano-hydroxyapatite particles

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