Nano-hybrid gradient scaffold for articular repair

Xu, Gan; Zhao, Yao; Geng, Yusheng; Cao, Shujun; Pan, Panpan; Wang, Jianhua; Chen, Jingdi

doi:10.1016/j.colsurfb.2021.112116

Cited by 14 publications

(6 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, there is a growing interest in stem cell therapy for KOA, due to the enormous differentiation potential and immunomodulatory biological properties of stem cells (46)(47)(48). Besides, previous studies have found that GO particle lubrication has a reparative effect on the KOA model established in rats, and that low concentrations of GO particles are biocompatible with umbilical cord MSCs (34,37), which promotes chondrogenic differentiation and osteogenic differentiation of human MSCs (35,(49)(50)(51). Hence, the anti-inflammatory effect of UCMSCs loaded with the GO particle lubricant on the papain-induced osteoarthritis model and the repair of articular cartilage and subchondral bone were evaluated in vivo in this study.…”

Section: Discussionmentioning

confidence: 99%

“…The scaffold cell complex structure of GO-loaded MSC provides sufficient contact with the defective cartilage, including the surrounding normal cartilage, thus repairing the damaged cartilage, cartilage matrix, and subchondral bone. In contrast to recent approaches that focus solely on the therapeutic effects of MSC, the holistic effect of the scaffold-cell combination is more emphasized (49)(50)(51). The combination of the two has great advantages in improving the biomechanical environment in the joint cavity, and it can be seen as a composite structure of the joint fluid-like action in the closed joint cavity structure, carrying solid lubricating particles with a restorative effect on the cells that modulate the inflammatory action (65).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Intra-Articular Injection of Umbilical Cord Mesenchymal Stem Cells Loaded With Graphene Oxide Granular Lubrication Ameliorates Inflammatory Responses and Osteoporosis of the Subchondral Bone in Rabbits of Modified Papain-Induced Osteoarthritis

et al. 2022

View full text Add to dashboard Cite

AimThis study is to investigate the effects of umbilical cord mesenchymal stem cells (UCMSCs) loaded with the graphene oxide (GO) granular lubrication on ameliorating inflammatory responses and osteoporosis of the subchondral bone in knee osteoarthritis (KOA) animal models.MethodsThe KOA animal models were established using modified papain joint injection. 24 male New Zealand rabbits were classified into the blank control group, GO group, UCMSCs group, and GO + UCMSCs group, respectively. The concentration in serum and articular fluid nitric oxide (NO), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), type II collagen (COL-II), and glycosaminoglycan (GAG) was detected using ELISA, followed by the dissection of femoral condyles and staining of HE and Micro-CT for observation via the microscope.ResultsGO granular lubrication and UCMSCs repaired the KOA animal models. NO, IL-6, TNF-α, GAG, and COL-II showed optimal improvement performance in the GO + UCMSCs group, with statistical significance in contrast to the blank group (P <0.01). Whereas, there was a great difference in levels of inflammatory factors in serum and joint fluid. Micro-CT scan results revealed the greatest efficacy of the GO + UCMSCs group in improving joint surface damage and subchondral bone osteoporosis. HE staining pathology for femoral condyles revealed that the cartilage repair effect in GO + UCMSCs, UCMSCs, GO, and blank groups were graded down.ConclusionUCMSCs loaded with graphene oxide granular lubrication can promote the secretion of chondrocytes, reduce the level of joint inflammation, ameliorate osteoporosis of the subchondral bone, and facilitate cartilage repair.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Intra-Articular Injection of Umbilical Cord Mesenchymal Stem Cells Loaded With Graphene Oxide Granular Lubrication Ameliorates Inflammatory Responses and Osteoporosis of the Subchondral Bone in Rabbits of Modified Papain-Induced Osteoarthritis

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The swelling rate method that was used is based on a previous report. [ 14 ] The CMC/CMCS‐TA/Ag hydrogel with a weight of W 1 was immersed in 15 mL PBS and incubated at 37 ℃. Then, the hydrogels were removed at 12, 24, 36, 48, and 60 h. The excess PBS liquid was removed with filter paper, and the weight was recorded as W 2 .…”

Section: Methodsmentioning

confidence: 99%

“…To overcome these problems, intermediate molecules were introduced to crosslink CMC molecules, thus cross-linking agents with high cytotoxic can be avoided. Carboxymethyl chitosan (CMCS) [14][15][16][17] is a derivative of chitosan [18] and can be considered an intermediate molecule for CMC cross-linking due to its many amino and carboxyl groups. Meanwhile, CMCS exhibits a wide-range of beneficial properties, such as good water solubility, antibacterial activity, hygroscopic moisture retention, and nontoxicity.…”

Section: Introductionmentioning

confidence: 99%

One‐Pot Preparation of Polysaccharide‐Based Antibacterial Hydrogel for Skin Wound Repair

Geng

et al. 2022

Macro Materials & Eng

Self Cite

View full text Add to dashboard Cite

Polysaccharide hydrogels are widely applied as wound dressings for skin wound repair due to their good biocompatibility and ability to regulate microenvironments. However, hydrogel application can result in skin wetting that can easily cause wound infections. Herein, a polysaccharide‐based antibacterial hydrogel that can promote skin wound repair is prepared by a one‐pot method. In this study, tannic acid (TA) is oxidized and cross‐linked with carboxymethyl chitosan (CMCS) through a Schiff base reaction to form the macromolecule CMCS‐TA. Carboxymethyl cellulose (CMC) cross‐links more easily with CMCS‐TA than CMCS when utilizing the cross‐linking agent carbondiimide hydrochloride (EDC). EDC has no cytotoxicity after hydrolysis and the hydrogel prepared by EDC cross‐linking has a low swelling rate. Due to the in situ reduction of Ag particles, the CMC/CMCS‐TA/Ag hydrogel exhibits good antibacterial properties against E. coli and S. aureus. Cytocompatibility tests and histological analysis indicate that the antibacterial hydrogel has good biocompatibility and can promote wound repair in a rat infection model. Therefore, the polysaccharide‐based hydrogel can be a promising wound dressing for skin wound repair.

show abstract

“…Nanostructured scaffold is a novel therapeutic approach for cartilage tissue regeneration with favorable biocompatibility, controllable degradation rate, suitable pore size and porosity and appropriate mechanical properties [ 128 ]. Scaffolds are primarily manufactured using different polymers, such as poly (lactic- co -glycolic acid) (PLGA), poly ( l -lactide) (PLLA) and poly (ε-caprolactone) (PCL), combined with functional groups by electrospinning, phase separation and self-assembly to facilitate cartilage repair ( Table 3 ).…”

Section: Nanomaterials For Bone-related Disease Therapymentioning

confidence: 99%