Nano-mechanical properties of individual mineralized collagen fibrils from bone tissue

Hang, Fei; Barber, Asa H.

doi:10.1098/rsif.2010.0413

Cited by 118 publications

(97 citation statements)

References 31 publications

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“…This failure mechanism appears to be a biological response to delocalize damage and maintain structural integrity during large deformation. Keeping the fibrillar organization could also favor pullout mechanisms, which represent yet another energy dissipation mechanism at the tissue scale, as observed in mineralized tendon49 or at bone fracture surfaces 14, 50, 51…”

Section: Resultsmentioning

confidence: 99%

“…Previous idealized models of nascent collagen fibrils have shown that mineral provides additional strength, stiffness, and ultimate strain to the fibrillar structure 31. However, these simplified two‐dimensional models ignore some geometric features of the fibril and do not completely capture the macroscopic deformation mechanisms as exposed in recent experimental work 14. Therefore, the mesoscale model of mineralized collagen fibril needs further improvements.…”

Section: Introductionmentioning

confidence: 99%

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Large Deformation Mechanisms, Plasticity, and Failure of an Individual Collagen Fibril With Different Mineral Content

Dépalle

Qin

Shefelbine

et al. 2015

Journal of Bone and Mineral Research

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Mineralized collagen fibrils are composed of tropocollagen molecules and mineral crystals derived from hydroxyapatite to form a composite material that combines optimal properties of both constituents and exhibits incredible strength and toughness. Their complex hierarchical structure allows collagen fibrils to sustain large deformation without breaking. In this study, we report a mesoscale model of a single mineralized collagen fibril using a bottom‐up approach. By conserving the three‐dimensional structure and the entanglement of the molecules, we were able to construct finite‐size fibril models that allowed us to explore the deformation mechanisms which govern their mechanical behavior under large deformation. We investigated the tensile behavior of a single collagen fibril with various intrafibrillar mineral content and found that a mineralized collagen fibril can present up to five different deformation mechanisms to dissipate energy. These mechanisms include molecular uncoiling, molecular stretching, mineral/collagen sliding, molecular slippage, and crystal dissociation. By multiplying its sources of energy dissipation and deformation mechanisms, a collagen fibril can reach impressive strength and toughness. Adding mineral into the collagen fibril can increase its strength up to 10 times and its toughness up to 35 times. Combining crosslinks with mineral makes the fibril stiffer but more brittle. We also found that a mineralized fibril reaches its maximum toughness to density and strength to density ratios for a mineral density of around 30%. This result, in good agreement with experimental observations, attests that bone tissue is optimized mechanically to remain lightweight but maintain strength and toughness. © 2015 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research (ASBMR).

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Large Deformation Mechanisms, Plasticity, and Failure of an Individual Collagen Fibril With Different Mineral Content

Dépalle

Qin

Shefelbine

et al. 2015

Journal of Bone and Mineral Research

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“…Experimental validation of the mechanical properties of bone at the nanoscale is also very challenging. Relevant experimental studies include atomic force microscopy measurements combined with electron microscopy imaging (SEM and TEM) (e.g., [58][59][60]) and tests on micropillars of bone at a µm scale [61][62][63]. Theoretical models can give valuable insights on bone's response at the nanoscale and the structure-property relations of bone in general.…”

Section: Modeling Of Bone At Nanoscale Various Geometric Modelsmentioning

confidence: 99%

The Ultrastructure of Bone and Its Relevance to Mechanical Properties

2017

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Bone is a biologically generated composite material comprised of two major structural components: crystals of apatite and collagen fibrils. Computational analysis of the mechanical properties of bone must make assumptions about the geometric and topological relationships between these components. Recent transmission electron microscope (TEM) studies of samples of bone prepared using ion milling methods have revealed important previously unrecognized features in the ultrastructure of bone. These studies show that most of the mineral in bone lies outside the fibrils and is organized into elongated plates 5 nanometers (nm) thick, ∼80 nm wide and hundreds of nm long. These so-called mineral lamellae (MLs) are mosaics of single 5 nm-thick, 20-50 nm wide crystals bonded at their edges. MLs occur either stacked around the 50 nm-diameter collagen fibrils, or in parallel stacks of 5 or more MLs situated between fibrils. About 20% of mineral is in gap zones within the fibrils. MLs are apparently glued together into mechanically coherent stacks which break across the stack rather than delaminating. ML stacks should behave as cohesive units during bone deformation. Finite element computations of mechanical properties of bone show that the model including such features generates greater stiffness and strength than are obtained using conventional models in which most of the mineral, in the form of isolated crystals, is situated inside collagen fibrils.

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“…Por desgracia, el repertorio de pruebas mecánicas, disponibles para pequeños huesos de animales, es limitado debido a las limitaciones inherentes impuestas por el tamaño del hueso (Hang & Barber, 2011).…”

Section: Discussionunclassified

Evaluación Biomecánica del Efecto de Bisfosfonatos y Aceite de Oliva en un Modelo de Remodelación Óseo en Fémur de Rata: Efectos en la Remodelación Ósea

Escudero

Virga

Aguzzi

et al. 2017

Int. J. Odontostomat.

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ESCUDERO, C.; VIRGA, C.; AGUZZI, A.; ARAMBURU, G. & DE LEONARDI, A.Evaluación biomecánica del efecto de bisfosfonatos y aceite de oliva en un modelo de remodelación óseo en fémur de rata. Efectos en la remodelación ósea. Int. J. Odontostomat., 11(1):19-24, 2017. RESUMEN:Estudios han demostrado que el aceite de oliva (O) con sus compuestos fenólicos tienen efectos positivos en diversos biomarcadores fisiológicos. Análisis previos han demostrado que los bisfosfonatos, son potentes inhibidores de la resorción ósea. Estudiar el efecto del tratamiento combinado de alendronato (AL) y pamidronato (PA) y de O sobre la regeneración ósea. Las fórmulas se dosificaron 0,5 mg/kg de peso para AL, y de 0,6 mg/kg de peso para PA. El O se administró en la dieta, 50 g/Kg. Cincuenta y cuatro ratas macho de la línea Wistar se dividieron en 6 grupos. El grupo control (C), recibió semanalmente 0,3 ml/100 g de solución salina vía subcutánea. El grupo (AL) recibió semanalmente por vía subcutánea en el miembro posterior izquierdo. El grupo (PA) se colocó igual que el grupo anterior. El grupo (O) fue tratado en la alimentación y en las áreas de la cirugía recibieron inyección subcutánea con solución fisiológica. El grupo (ALO) recibió tratamiento combinado con AL y O. El grupo (PAO) se trató igual al anterior. Se obtuvieron muestras de fémur en tiempos 15, 30, 60 y 90 días, que se incluyeron en solución fisiológica y mantenidos a -20 ºC. Los estudios estadísticos se realizaron a través del análisis de la variancia a dos y tres criterios de clasificación. Sólo el factor días influye significativamente sobre los valores. Las diferencias entre drogas no resultaron estadísticamente significativas. Tampoco se verificó interacción significativa entre los factores Droga y etapa. Los valores más elevados de fuerza de ruptura aplicada, se registraron a los 90 días. No se encontraron diferencias significativas en los ensayos biomecánicos, poniendo de manifiesto la acción sistémica de los fármacos. Estas acciones fueron benéficas al aumentar la rigidez.PALABRAS CLAVE: bisfosfonatos, aceite de oliva, ensayos biomecánicos. INTRODUCCIÓNLa mecánica y la ciencia de materiales estudian los efectos y la relación entre las fuerzas aplicadas sobre una estructura o cuerpo rígido y la deformación producida (Buehler, 2008). El hueso, para su estudio, se puede considerar tanto un tejido como una estructura, ya que desempeña dos funciones básicas: control del metabolismo de Ca, P y Mg (función fisiológi-ca) y soporte del organismo y protección de órganos (función mecánica). La complejidad mecánica del tejido óseo, compuesto de hueso cortical y hueso trabecular, ambos con comportamientos mecánicos distintos, supera la de la mayoría de los materiales utilizados en ingeniería (Eppell et al., 2006). Para mejorar los tratamientos aplicados contra enfermedades osteodegenerativas, como es el caso de la osteoporosis, resulta imprescindible optimizar las técnicas de diagnóstico que se basan principalmente en establecer correlaciones entre las variables biomecánicas y las...

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Nano-mechanical properties of individual mineralized collagen fibrils from bone tissue

Cited by 118 publications

References 31 publications

Large Deformation Mechanisms, Plasticity, and Failure of an Individual Collagen Fibril With Different Mineral Content

Large Deformation Mechanisms, Plasticity, and Failure of an Individual Collagen Fibril With Different Mineral Content

The Ultrastructure of Bone and Its Relevance to Mechanical Properties

Evaluación Biomecánica del Efecto de Bisfosfonatos y Aceite de Oliva en un Modelo de Remodelación Óseo en Fémur de Rata: Efectos en la Remodelación Ósea

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